Blood and Tissue Selenium Determination by Hydride Generation Atomic Absorption Spectrophotometry

Pechová, Alena; Pavlata, L.; Illek, J.

doi:10.2754/avb200574040483

Cited by 33 publications

(28 citation statements)

References 12 publications

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“…After removal of nitric acid from the digested sample in the evaporation rotor of the same apparatus, the water solution was mixed with 20% hydrochloric acid. Selenium in the processed sample was measured using HG-AAS (Hydride Generation Atomic Absorption Spectrometry) according to the method described by Pechova et al (2005) in an AAS Solaar M6 (Unicam, Great Britain). Selenium concentrations were expressed in terms of µg/l of whole blood.…”

Section: Methodsmentioning

confidence: 99%

Direct and indirect assessment of selenium status in sheep - a comparison

Pavlata¹,

Mišurová²,

Pechová³

et al. 2012

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to determine the relationship between selenium concentration and activity of glutathione peroxidase (GSH-Px) in the whole blood of sheep and reference ranges for the activity of GSH-Px for evaluation of the selenium status of sheep in the Czech Republic. Selenium concentration and activity of glutathione peroxidase were determined in whole blood samples collected from 92 sheep in six herds. The GSH-Px activity in the samples was measured using the photometric method. Selenium in the sample was measured using the hydride technique atomic absorption spectrometry. Data on both parameters were processed using correlation and regression analysis in order to obtain reference values of GSH-Px for the indirect evaluation of the selenium status of sheep. The two variables showed a close and significant correlation (r = 0.95; P < 0.01). The regression line, defined by the equation y = 7.5857x -121.87 (linear) or y = -0.0167x 2 + 11.993x -355.57 (polynomial), allowed us to determine the GSH-Px activity of 637, resp. 677 μkat/l as equivalent to selenium concentration in whole blood of sheep 100 μg/l. Mean selenium concentration and mean GSH-Px activity found in whole blood samples were 123.42 ± 57.84 μg/l and 814.34 ± 463.64 μkat/l, respectively. In this study, a close dependence of GSH-Px activity on the selenium concentration in the blood of sheep was found. Activity values of GSH-Px were determined for use in the diagnosis of sufficient selenium status in sheep in the Czech Republic (GSH-Px activity greater than 600 μkat/l of whole blood).

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Section: Methodsmentioning

confidence: 99%

Direct and indirect assessment of selenium status in sheep - a comparison

Pavlata¹,

Mišurová²,

Pechová³

et al. 2012

Vet. Med. - Czech

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“…Milk and whole blood concentration of Se was determined after mineralization of samples by hydride technique using Solaar M6 (Unicam, Great Britain) with electrothermically heated cuvette as per methodology designed by Pechová et al (2005). All analyses were performed by the laboratory of the Clinic of Ruminant Diseases of the University of Veterinary and Pharmaceutical Sciences Brno.…”

Section: Methodsmentioning

confidence: 99%

Contents of Zn, Cu, Mn and Se in Milk in Relation to their Concentrations in Blood, Milk Yield and Stage of Lactation in Dairy Cattle

et al. 2008

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The objective of the study was to assess the effect of actual daily milk production and lactation stage on concentrations of Zn, Mn, Cu and Se in milk, and monitor correlations between milk and blood concentrations of these microelements.The study was performed in a herd of Holstein cattle with the average milk yield of 8,562 kg. Thirty-five dairy cows housed in one group were included in the study. Blood and milk samples were taken during two separate milk yield checks done 4 weeks apart. Actual milk production of monitored cows ranged from 19.6 to 62.6 l daily. For lactation stages we evaluated results of examinations performed from 7 to 188 days of lactation.Blood examinations showed that the cows included in our study had good supplementation with the microelements in question. Milk concentrations of individual microelements were as follows: 3855.2  814.7 μg/l of Zn; 36.3  14.4 μg/l of Cu; 20.1  8.3 μg/l of Mn, and 28.6  7.1 μg/l of Se. The effect of daily milk production on milk concentrations of the microelements was identified only for copper (r = -0.302, p  0.05). The variable of days of lactation (not considering days of the colostrum period) showed a positive correlation in manganese (r = 0.419, p  0.01); copper and selenium showed negative correlations (Cu: r = -0.258, p  0.05; Se: r = -0.277, p  0.05). The daily milk production influenced negatively only Cu concentration in milk, but Se, Zn and Mn was not influenced.With advancing lactation after colostrum period the concentration of Mn in milk raised, the concentration of Cu and Se declined and the concentration of Zn was unchanged. Cows, microelements, blood plasma

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“…After vaporization and conversion of the mineralizate into water solution, 20% HCl is added. In such a solution, selenium content is determined in AAS Solaar M6 unit by Unicam (Great Britain) using the hydride technique of atomic absorption spectrophotometry (Pechova et al, 2005).…”

Section: Methodsmentioning

confidence: 99%

Selenium metabolism in goats - maternal transfer of selenium to newborn kids

Mišurová¹,

Pavlata²,

Pechová³

et al. 2009

Vet. Med. - Czech

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AbStrAct:The aim of our study was to compare the concentration of selenium (Se) and the activity of glutathione peroxidase (GSH-Px) in the whole blood of goats and their newborn kids. The experiment involved 25 gravid, clinically healthy goats of the white shorthaired breed. On the day of delivery, we took whole blood from the mother and her newborn kid before the kid drank the first colostrum. In mothers, the measured average concentration of Se in whole blood was 149.60 ± 45.01 μg/l, the average concentration of Se in kids was 87.91 ± 29.66 μg/l. Average activity of GSH-Px in the blood of mothers was 938.46 ± 341.09 μkat/l, and in the blood of kids 658.20 ± 339.13 μkat/l. Regression and correlation analysis produced regression line formulas and correlation coefficients that revealed a close, statistically significant relation (p < 0.01) between the concentration of Se in the blood of mothers and their kids and the activity of GSH-Px in mothers and their kids. The relation between the concentration of Se in the blood (µg/l) of mothers and kids was y = 0.484x + 15.55; r = 0.73, the relation between the activity of GSH-Px in blood (µkat/l) of mothers and their kids was y = 0.809x -101.27; r = 0.80. The concentration of Se and activity of GSH-Px was lower in newborn kids than in their mothers, reaching approximately 60-70% of the mother's levels. The relation between the concentration of Se and activity of GSH-Px in the blood of goats was y = 4.23x + 276.31; r = 0.64 (p < 0.01) and the relation between the concentration of Se and the activity of GSH-Px in the blood of kids was y = 6.556x + 64.70; r = 0.83 (p < 0.01). It follows that a Se concentration of 100 μg/l corresponds to a GSH-Px activity of 699.51 μkat/l in the blood of mothers and 720.34 μkat/l in the blood of kids. The results show the need to provide for a sufficient Se saturation of goats with a view to preventing Se deficiency in kids and that the Se concentration in the blood of newborn kids is physiologically about 40% lower in comparison with the Se concentration in the blood of their mothers. This should be taken into account when interpreting the results and assessing the reference values of Se concentration in the blood of kids.

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Blood and Tissue Selenium Determination by Hydride Generation Atomic Absorption Spectrophotometry

Cited by 33 publications

References 12 publications

Direct and indirect assessment of selenium status in sheep - a comparison

Direct and indirect assessment of selenium status in sheep - a comparison

Contents of Zn, Cu, Mn and Se in Milk in Relation to their Concentrations in Blood, Milk Yield and Stage of Lactation in Dairy Cattle

Selenium metabolism in goats - maternal transfer of selenium to newborn kids

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