Blood and clonogenic hemopoietic cells of newts after the space flight

Michurina, Tatyana V.; Domaratskaya, E. I.; Nikonova, T. M.; Khrushchov, N. G.

doi:10.1016/0273-1177(95)00650-4

Cited by 15 publications

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“…During the past 40 years of human spaceflight, it has been confirmed that exposure to microgravity during the expeditions affects almost all human physiological systems; these changes are some of the main obstacles that have prevented human interplanetary missions (Sonnenfeld 1999; Turner 2000; West 2000; Crawford‐Young 2006). Based on the development of space medicine and bioastronautics, research has identified that the changes in activity and differentiation of tissue cells are the leading causes of physiological changes (Weinreb 1994; Michurina et al . 1996; Carmeliet et al .…”

Section: Introductionmentioning

confidence: 99%

Simulated microgravity inhibits the proliferation and osteogenesis of rat bone marrow mesenchymal stem cells

et al. 2007

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Section: Introductionmentioning

confidence: 99%

Simulated microgravity inhibits the proliferation and osteogenesis of rat bone marrow mesenchymal stem cells

et al. 2007

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“…1, Haidian District, Beijing 100094, China. Tel. : +86-10-62898668 or +86-13501332556; Fax: +86-10-62898668; E-mail: yiunghuidd@vip.sina.com medicine and bioastronautics, research has identified that the changes in activity and differentiation of tissue cells are the leading causes of physiological changes (Weinreb 1994;Michurina et al . 1996;Carmeliet et al .…”

Section: Introductionmentioning

confidence: 99%

Simulated microgravity inhibits the proliferation and osteogenesis of rat bone marrow mesenchymal stem cells

et al. 2008

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“…One of the key differences between the environments in space and on Earth is microgravity. It has been confirmed that exposure to microgravity has various effects on astronauts' physiology, including bone loss, anemia, muscle atrophy, and immune alterations (1)(2)(3)(4), especially during long-term spaceflight. Some studies revealed that microgravity results in ;1-2% osteopenia per month (5,6).…”

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confidence: 97%

Space microgravity drives transdifferentiation of human bone marrow‐derived mesenchymal stem cells from osteogenesis to adipogenesis

Zhang

Jiang

et al. 2018

FASEB j.

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Bone formation is linked with osteogenic differentiation of mesenchymal stem cells (MSCs) in the bone marrow. Microgravity in spaceflight is known to reduce bone formation. In this study, we used a real microgravity environment of the SJ-10 Recoverable Scientific Satellite to examine the effects of space microgravity on the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs). hMSCs were induced toward osteogenic differentiation for 2 and 7 d in a cell culture device mounted on the SJ-10 satellite. The satellite returned to Earth after going through space experiments in orbit for 12 d, and cell samples were harvested and analyzed for differentiation potentials. The results showed that space microgravity inhibited osteogenic differentiation and resulted in adipogenic differentiation, even under osteogenic induction conditions. Under space microgravity, the expression of 10 genes specific for osteogenesis decreased, including collagen family members, alkaline phosphatase ( ALP), and runt-related transcription factor 2 ( RUNX2), whereas the expression of 4 genes specific for adipogenesis increased, including adipsin ( CFD), leptin ( LEP), CCAAT/enhancer binding protein β ( CEBPB), and peroxisome proliferator-activated receptor-γ ( PPARG). In the analysis of signaling pathways specific for osteogenesis, we found that the expression and activity of RUNX2 was inhibited, expression of bone morphogenetic protein-2 ( BMP2) and activity of SMAD1/5/9 were decreased, and activity of focal adhesion kinase (FAK) and ERK-1/2 declined significantly under space microgravity. These data indicate that space microgravity plays a dual role by decreasing RUNX2 expression and activity through the BMP2/SMAD and integrin/FAK/ERK pathways. In addition, we found that space microgravity increased p38 MAPK and protein kinase B (AKT) activities, which are important for the promotion of adipogenic differentiation of hMSCs. Space microgravity significantly decreased the expression of Tribbles homolog 3 ( TRIB3), a repressor of adipogenic differentiation. Y15, a specific inhibitor of FAK activity, was used to inhibit the activity of FAK under normal gravity; Y15 decreased protein expression of TRIB3. Therefore, it appears that space microgravity decreased FAK activity and thereby reduced TRIB3 expression and derepressed AKT activity. Under space microgravity, the increase in p38 MAPK activity and the derepression of AKT activity seem to synchronously lead to the activation of the signaling pathway specifically promoting adipogenesis.-Zhang, C., Li, L., Jiang, Y., Wang, C., Geng, B., Wang, Y., Chen, J., Liu, F., Qiu, P., Zhai, G., Chen, P., Quan, R., Wang, J. Space microgravity drives transdifferentiation of human bone marrow-derived mesenchymal stem cells from osteogenesis to adipogenesis.

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Blood and clonogenic hemopoietic cells of newts after the space flight

Cited by 15 publications

References 0 publications

Simulated microgravity inhibits the proliferation and osteogenesis of rat bone marrow mesenchymal stem cells

Simulated microgravity inhibits the proliferation and osteogenesis of rat bone marrow mesenchymal stem cells

Simulated microgravity inhibits the proliferation and osteogenesis of rat bone marrow mesenchymal stem cells

Space microgravity drives transdifferentiation of human bone marrow‐derived mesenchymal stem cells from osteogenesis to adipogenesis

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