Blood amino acid levels in patients with insulin excess (functioning insulinoma) and insulin deficiency (diabetic ketosis)

Berger, Michael; Zimmermann-Telschow, H.; Berchtold, Peter; Drost, H.; Müller, W.; Gries, F. A.; Zimmermann, H.

doi:10.1016/0026-0495(78)90214-7

Cited by 62 publications

(34 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is suggested, therefore, that this alteration of the pattern ofplasma amino acids is a consequence ofglucagon deficiency. On the other hand, absence of significant elevation of branched chain amino acids, leucine, isoleucine, and valine, suggests that insulin deficiency in our patients was relatively modest (30,31). Only after 3 d of insulin withdrawal (patient 2) did the branched chain amino acids substantially increase (Table II).…”

Section: Discussionmentioning

confidence: 62%

“…The patient felt well, was ambulatory around the ward, and was able to take her meals. Plasma glucose was 290 mg/dl 24 h after the last insulin injection, rose to 390 mg/dl after 48 h, and remained at approximately this level up to 72 h. As shown in Table II, there was moderate ketosis by 72 h, whereas the concentration of branched chain amino acids rose quite markedly, an indication of insulin deficiency (30,31). IRG, however, remained constant at about 570 pg/ml and, again, failed to rise in response to arginine infusion.…”

Section: Resultsmentioning

confidence: 94%

See 1 more Smart Citation

Glucagon Immunoreactivities and Amino Acid Profile in Plasma of Duodenopancreatectomized Patients

Müller¹,

Berger²,

Suter³

et al. 1979

J. Clin. Invest.

Self Cite

View full text Add to dashboard Cite

A B S T R A C T Glucogon immunoreactivity (IRG) was measured in plasma of duodenopancreatectomized subjects with a nonspecific (K-4023) and a specific (30-K) glucagon antiserum. After an overnight fast, plasma IRG (K-4023) was significantly (P < 0.05) higher in the subjects without pancreas, averaging 782±79 (SEM) pgeq/ml, than in the controls (482±80 pgeq/ml). IRG (30-K) of 162±68 pglml did not change during an infusion ofarginine (450 mg/kg per 40 min). Insulin deprivation during 3 d in one patient did not restore the IRG response to arginine as reported in depancreatized dogs.Bio-Gel P-30 column chromatography revealed that virtually all IRG (30-K) measured in whole plasma was of different molecular weight than glucagon, and primarily of a mol wt 2 40,000. Intravenous arginine did not significantly alter the chromatographic pattern of these plasmas. Thus, as postulated by others, duodenopancreatectomized humans have virtually no circulating 3,500-dalton glucagon. Hence, the presence of 3,500-dalton glucagon in plasma is not a condition for the diabetic state. It might, nevertheless, when present in normal or excessive amounts, worsen the metabolic state of diabetic patients.Among 14 amino acids measured in plasma of these patients, the concentrations of alanine, serine, ornithine, and arginine were significantly (P < 0.05) elevated to approximately twice that of normal: alanine and serine are both substrates for gluconeogenesis, whereas ornithine and arginine are involved in the formation of urea, the second product of hepatic gluco-

show abstract

Section: Discussionmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 94%

Glucagon Immunoreactivities and Amino Acid Profile in Plasma of Duodenopancreatectomized Patients

Müller¹,

Berger²,

Suter³

et al. 1979

J. Clin. Invest.

Self Cite

View full text Add to dashboard Cite

show abstract

“…This disturbance is due to the role exerted by insulin in AA transport through cell membranes [23, 24, 25, 26]. We postulate that a chronic elevation in the plasma level of some AAs could play a role in the high vasopressin secretion of diabetes.…”

Section: Vasopressin Elevation In Diabetes Mellitusmentioning

confidence: 99%

Vasopressin and Diabetes mellitus

Bankir¹,

Bardoux²,

Ahloulay³

2001

Nephron

100

View full text Add to dashboard Cite

In diabetes mellitus (DM), the urine flow rate is increased, and the fluid turnover in the body is accelerated because of the glucose-induced osmotic diuresis. On the other hand, plasma vasopressin (VP) is elevated in both type 1 and type 2 DM. This elevation seems to be due to a resetting of the osmostat. A high VP level is beneficial in the short term because it limits to some extent the amount of water required for the excretion of a markedly enhanced load of osmoles (mainly glucose). However, in the long run, it may have adverse effects by favoring the developement of diabetic nephropathy. VP has been shown in normal rats to induce kidney hypertrophy, glomerular hyperfiltration, and an increase in urinary albumin excretion (features also occurring in association in the period preceding diabetic nephropathy). Moreover, VP has been shown to participate in the progression of renal failure in rats with five-sixths reduction in renal mass. In recent studies, we have shown (1) that creatinine clearance, albuminuria and renal mass increased much less during experimental DM in Brattleboro rats unable to secrete VP than in their VP-replete Long-Evans controls, and (2) that albuminuria was prevented during experimental DM in Wistar rats when a VP nonpeptidic, highly selective V2 receptor antagonist was administered chronically for 9 weeks. Taken together, these results strongly suggest that VP plays a crucial role in the onset and aggravation of the renal complications of DM. The mechanisms by which VP exerts these adverse V2-dependent effects are not yet elucidated. They are most likely indirect and may involve several intermediate steps comprising VP-induced changes in the composition of the tubular fluid in the loop of Henle (due to solute recycling in the renal medulla associated with improved concentrating activity of the kidney), inhibition of the tubuloglomerular feedback control of glomerular function, and alterations in glomerular hemodynamics by the intrarenal renin-angiotensin system.

show abstract

“…Rats made diabetic by alloxan or streptozotocin injection exhibit not only de creased level of plasma tryptophan and in creased concentration of the branchcd-chain amino acids, but also the diabetic animals required a higher dose of L-tryptophan to induce the same changes in brain tryptophan usually produced by a much smaller dose in normal rats [24,25]. The cause of this dimin ished responsiveness of brain tryptophan may be due to the increased blood levels of branched-chain amino acids present in the diabetic, which enhance competition with tryptophan for its brain uptake [3,28],…”

Section: Introductionmentioning

confidence: 97%

Role of 5-Hydroxytryptamine in the Regulation of Brain Neuropeptides in Normal and Diabetic Rat

1986

View full text Add to dashboard Cite

The effect of 5-hydroxytryptamine (5-HT) alteration on brain dopamine (DA), norepinephrine (NE), β-endorphin (βE) and immunoreactive insulin (IRI) was studied in Sprague-Dawley diabetic and control rats. Diabetes was induced using alloxan (45 mg/kg), 15 days prior to sacrificing. Both control and diabetic animals were treated with either p-chlorophenylalanine (PCPA, 300 mg/kg) 3 days prior to sacrificing or fluoxetine (10 mg/kg) twice daily for 3 days. PCPA treatment significantly decreased brain content of 5-HT and 5-hydroxyindole acetic acid (5-HIAA) while it caused significant increase and decrease in brain βE and insulin levels, respectively, in both normal and diabetic rat. Meanwhile, the administration of fluoxetine resulted in significant increase in brain content of 5-HT, DA, NE and insulin but significant decline of βE in diabetic and saline control rats. The results of this experiment indicate that 5-HT may be regulating both βE and insulin regardless of the availability of pancreatic insulin.

show abstract

Blood amino acid levels in patients with insulin excess (functioning insulinoma) and insulin deficiency (diabetic ketosis)

Cited by 62 publications

References 18 publications

Glucagon Immunoreactivities and Amino Acid Profile in Plasma of Duodenopancreatectomized Patients

Glucagon Immunoreactivities and Amino Acid Profile in Plasma of Duodenopancreatectomized Patients

Vasopressin and Diabetes mellitus

Role of 5-Hydroxytryptamine in the Regulation of Brain Neuropeptides in Normal and Diabetic Rat

Contact Info

Product

Resources

About