Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate

Huang, Ruijia; Zhang, Ke; Zhu, Guoshuai; Sun, Zhencheng; He, Songliang; Chen, Wenwen

doi:10.3390/s18103537

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…During the pandemic of infectious diseases, efficient tests can significantly slow down the spread of diseases and improve public health decision-making. Decentralizing the complex biomarker assay from laboratory sites to remote areas and temporary stations with sample-to-answer point-of-care-test (POCT) devices will greatly improve the testing efficiency [ 1 , 2 , 3 , 4 ]. Antibody-based immunoassays have achieved extraordinary success in the sensing of biomarkers during the past for more than half a century [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Alginate Hydrogel-Embedded Capillary Sensor for Quantitative Immunoassay with Naked Eye

Zheng

Gao

Shen

et al. 2020

Sensors

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We have developed an alginate hydrogel-embedded capillary sensor (AHCS) for naked eye-based quantification of immunoassay. Alkaline phosphatase (ALP) can modulate gel-sol transformation to increase the permeability of Cu2+-cross-linked alginate hydrogel film in the AHCS, followed by solution exchange into the capillary. Through measuring the length of the liquid phase of the microfluidics in the capillary at a given time, the concentration of the ALP could be quantified with the naked eye. Since ALP is widely applied as a signal reporter for immunoassays, the AHCS could easily accommodate conventional immune sensing platforms. We justify the practicality of AHCS with hepatitis B virus surface antigen (HBsAg) in serum samples and got comparable results with commercialized immunoassay. This AHCS is easy to make and use, effective in cost, and robust in quantification with the naked eye, showing great promise for next generation point-of-care testing.

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Section: Introductionmentioning

confidence: 99%

Alginate Hydrogel-Embedded Capillary Sensor for Quantitative Immunoassay with Naked Eye

Zheng

Gao

Shen

et al. 2020

Sensors

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“…Many studies have demonstrated that gold nanoparticles have a signal amplification effect on biochemical detection. ELISA modified with gold nanoparticle layers (GNPL) are more sensitive than conventional commercialized ELISA kits in carcinoembryonic antigen detection [11,12].…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Detection of PCV2 Based on Tyramide Signals and GNPL Amplification

Zhang

Xia

et al. 2019

Molecules

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The frequent emergence of secondary infection and immunosuppression after porcine circovirus type 2 (PCV2) infection highlights the need to develop sensitive detection methods. A dual-signal amplification enzyme-linked immunosorbent assay (ELISA) based on a microplate coated with gold nanoparticle layers (GNPL) and tyramide signal amplification (TSA) was established. Results confirmed that the microplates coated with GNPL have a strong binding ability to the antibody without affecting the biological activity of the antibody. The microplates coated with GNPL have strong binding ability to the antibody, and the amplification of the tyramide signal is combined to further improve the sensitivity of PCV2. The PCV2 antibody does not crossreact with other viruses, demonstrating that the method has good specificity. A dual-signal amplification strategy is developed using microplates modified with GNPL and TSA to sensitively detect PCV2.

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Kaempferol inhibits the cell migration of human hepatocellular carcinoma cells by suppressing MMP‐9 and Akt signaling

Chen

et al. 2021

Environmental Toxicology

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Metastasis is the most prevalent cause of cancer‐related deaths and treatment failure in patients with hepatocellular carcinoma (HCC). Kaempferol is a natural flavonol belonging to the subgroup of flavonoids and exhibits potent anticancer activities. This study provides molecular evidence on the anti‐invasive and anti‐migratory effects of kaempferol on human HCC cells. The anti‐invasive effect was investigated by applying kaempferol on two human HCC cell lines (Huh‐7 and SK‐Hep‐1). Kaempferol reduced the invasion and migration of Huh‐7 and SK‐Hep‐1 cells by Boyden chamber invasion assay and wound healing assay, respectively. A protease array analysis showed that Matrix Metalloproteinase‐9 (MMP‐9) was dramatically downregulated in HCC cells after kaempferol treatment. Gelatin zymography and Western blot assay showed that kaempferol reduced the activities and protein expression of MMP‐9, respectively. Kaempferol also sufficiently suppressed the phosphorylation of the Akt expression. Overall, kaempferol inhibited the invasive properties of human HCC cells by targeting MMP‐9 and Akt pathways. Hence, kaempferol could be used as an adjuvant therapeutic agent for the treatment of human HCC cells.

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Blocking-Free ELISA Using a Gold Nanoparticle Layer Coated Commercial Microwell Plate

Cited by 6 publications

References 28 publications

Alginate Hydrogel-Embedded Capillary Sensor for Quantitative Immunoassay with Naked Eye

Alginate Hydrogel-Embedded Capillary Sensor for Quantitative Immunoassay with Naked Eye

Highly Sensitive Detection of PCV2 Based on Tyramide Signals and GNPL Amplification

Kaempferol inhibits the cell migration of human hepatocellular carcinoma cells by suppressing MMP‐9 and Akt signaling

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