Blocking amino acid transporter <i>Os<scp>AAP</scp>3</i> improves grain yield by promoting outgrowth buds and increasing tiller number in rice

Lü, Kai; Wu, Bowen; Jie, Wang; Zhu, Wei; Nie, Haipeng; Qian, Junjie; Huang, Weiting; Fang, Zhongming

doi:10.1111/pbi.12907

Cited by 192 publications

(151 citation statements)

References 52 publications

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“…The molecular functions of several amino acid transporters (AATs) are characterized in Arabidopsis and rice. For instance AtAAPs transport neutral and charged amino acids (Fischer et al ., ; Lee et al ., ), and AtANTs are involved in transporting aromatic and neutral amino acids (Hirner et al ., ); OsAAPs can transport neutral, acidic and basic amino acids (Lu et al ., ; Peng et al ., ; Taylor et al ., ). These findings suggest that different types of AATs are able to transport a broad spectrum of neutral amino acids.…”

Section: Discussionmentioning

confidence: 99%

NRT1.1B improves selenium concentrations in rice grains by facilitating selenomethinone translocation

Zhang

Deng

et al. 2019

Plant Biotechnology Journal

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Summary Selenium (Se) is an essential trace element for humans and other animals, yet approximately one billion people worldwide suffer from Se deficiency. Rice is a staple food for over half of the world's population that is a major dietary source of Se. In paddy soils, rice roots mainly take up selenite. Se speciation analysis indicated that most of the selenite absorbed by rice is predominantly transformed into selenomethinone (SeMet) and retained in roots. However, the mechanism by which SeMet is transported in plants remains largely unknown. In this study, SeMet uptake was found to be an energy‐dependent symport process involving H + transport, with neutral amino acids strongly inhibiting SeMet uptake. We further revealed that NRT1.1B, a member of rice peptide transporter (PTR) family which plays an important role in nitrate uptake and transport in rice, displays SeMet transport activity in yeast and Xenopus oocyte. The uptake rate of SeMet in the roots and its accumulation rate in the shoots of nrt1.1b mutant were significantly repressed. Conversely, the overexpression of NRT1.1B in rice significantly promoted SeMet translocation from roots to shoots, resulting in increased Se concentrations in shoots and rice grains. With vascular‐specific expression of NRT1.1B , the grain Se concentration was 1.83‐fold higher than that of wild type. These results strongly demonstrate that NRT1.1B holds great potential for the improvement of Se concentrations in grains by facilitating SeMet translocation, and the findings provide novel insight into breeding of Se‐enriched rice varieties.

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Section: Discussionmentioning

confidence: 99%

NRT1.1B improves selenium concentrations in rice grains by facilitating selenomethinone translocation

Zhang

Deng

et al. 2019

Plant Biotechnology Journal

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“…). In a previous study, the elevated expression of OsAAP3 in transgenic plants resulted in a significant increase amino acid concentrations and inhibited bud outgrowth and rice tillering ( Lu et al., ). The results suggest that CsAAP3 is involved in N utilization in the shoot development of tea plants.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of nitrogen translocation from mature leaves to new shoots and related gene expression during spring shoots development in tea plants (Camellia sinensis L.)

Fan

Zhang

Tang

et al. 2020

J. Plant Nutr. Soil Sci.

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The contribution of N remobilization is crucial for new shoots growth and quality formation during spring tea shoots development. However, the translocation mechanism of N from source leaves to sink young shoots is not well understood. In the present study, 15N urea was applied to mature tea leaves one week before bud break to track N remobilization in a field experiment. The dynamic changes in plant 15N abundance, contents of amino acids, and the expression levels of genes related to N metabolism and translocation were followed during the 18‐d development of new spring shoots until three expanding young leaves. The results showed that during the growth of new shoots the amount of 15N in the shoots increased, whereas the Ndff (N derived from 15N‐urea) in mature leaves decreased, showing that the foliar‐applied N in mature leaves was readily exported to new shoots. This process was found to be accompanied by decline of chlorophylls. In the mature leaves, expression CsATG18a and CsSAG12 involved in autophagy was dramatically induced (> 4‐fold) at approximately nine days after the bud breaking. The genes involved in the transformation of amino acids, including primarily CsGDH2, CsGDH4, CsGLT3, CsGS1;3, and CsASN2 were upregulated by > 3‐fold after bud breaking. The expression levels of CsATG8A, CsATG9, CsSAG12, CsGS1;1, CsGDH1, and CsAAP6 correlated negatively with the Ndff in mature leaves, but positively with 15N amount and total N amount in new shoots, suggesting these genes played important roles in N export from mature leaves. In the new shoots, the expression of most genes showed two defined peaks, one on six days and one on 12 days after bud breaking. The expression of CsGS2, CsASN3, CsGLT1, and CsAAP4 positively correlated with the 15N amount and total N amount in new shoots. These genes might be involved in the transport and re‐assimilation of N from mature leaves. The overall results demonstrated that the translocation of 15N from mature leaves to new spring shoots was regulated by the genes involved in autophagy, protein degradation, amino acid transformation and transport.

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“…The content of free amino acids was determined as described previously [54], with slight modifications. Specifically, approximately 0.1 g of leaf sample was added into a tube containing 1 mL of 80% ethanol, and the tube was placed in a water bath for 20 min at 80 • C. This procedure was repeated twice, and the extract was placed in an oven at 80 • C until the ethanol was completely evaporated.…”

Section: Free Amino Acid Determinationmentioning

confidence: 99%

Integrated Transcriptional and Proteomic Profiling Reveals Potential Amino Acid Transporters Targeted by Nitrogen Limitation Adaptation

Liao

Tang

Zhou

et al. 2020

IJMS

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Nitrogen (N) is essential for plant growth and crop productivity. Organic N is a major form of remobilized N in plants’ response to N limitation. It is necessary to understand the regulatory role of N limitation adaption (NLA) in organic N remobilization for this adaptive response. Transcriptional and proteomic analyses were integrated to investigate differential responses of wild-type (WT) and nla mutant plants to N limitation and to identify the core organic N transporters targeted by NLA. Under N limitation, the nla mutant presented an early senescence with faster chlorophyll loss and less anthocyanin accumulation than the WT, and more N was transported out of the aging leaves in the form of amino acids. High-throughput transcriptomic and proteomic analyses revealed that N limitation repressed genes involved in photosynthesis and protein synthesis, and promoted proteolysis; these changes were higher in the nla mutant than in the WT. Both transcriptional and proteomic profiling demonstrated that LHT1, responsible for amino acid remobilization, were only significantly upregulated in the nla mutant under N limitation. These findings indicate that NLA might target LHT1 and regulate organic N remobilization, thereby improving our understanding of the regulatory role of NLA on N remobilization under N limitation.

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Blocking amino acid transporter OsAAP3 improves grain yield by promoting outgrowth buds and increasing tiller number in rice

Cited by 192 publications

References 52 publications

NRT1.1B improves selenium concentrations in rice grains by facilitating selenomethinone translocation

NRT1.1B improves selenium concentrations in rice grains by facilitating selenomethinone translocation

Dynamics of nitrogen translocation from mature leaves to new shoots and related gene expression during spring shoots development in tea plants (Camellia sinensis L.)

Integrated Transcriptional and Proteomic Profiling Reveals Potential Amino Acid Transporters Targeted by Nitrogen Limitation Adaptation

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