Blockage of voltage-gated calcium signaling impairs migration of glial cells in vivo

Lohr, Christian; Heil, Jan E.; Deitmer, Joachim W.

doi:10.1002/glia.20163

Cited by 41 publications

(69 citation statements)

References 58 publications

(50 reference statements)

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“…The L-type calcium-channel blocker, Verapamil, had been shown at high concentrations (5mM) to inhibit the migration of neuropil glial cells in M. sexta (Lohr, 2005). 5mM Verapamil was applied acutely and reduced the amplitude of the inward current by ~2/3 and washout reversed most of the reduction (not shown).…”

Section: Glial Cell Electrophysiology During the Period Of Axon Ingrowthmentioning

confidence: 92%

“…Because glial migration is a necessary part of the process of network formation in the antennal nerve and because the migration of the central glial cells has specifically been shown in the moth olfactory pathway to be calcium-dependent (Lohr et al, 2005), we examined the effect of blocking the R-like current on glial network formation.…”

Section: Role Of Calcium Currents and Effect Of Blocking Neuronal Actmentioning

confidence: 99%

“…Calcium transients in glial cells have been reported many systems, including olfactory ensheathing cells in the mammalian olfactory bulb (Reiger et al, 2007), astrocytes in the cortex (van den Pol et al, 1992), and neuropil glial cells in the olfactory (antennal) lobe of the moth and in the giant glial cells of medicinal leech ganglia (Lohr et al, 2005;Lohr and Deitmer, 2006).…”

Section: Calcium Current In the An Glial Cellsmentioning

confidence: 99%

“…In the latter, calcium transients begin to appear at stage 6, and are present in all NP glial cells by stage 7; stage 6 is the stage at which NP glial cells begin to migrate to surround and stabilize developing glomeruli (Lohr et al, 2005;Lohr and Deitmer, 2006;Bauman et al, 1996).…”

Section: Calcium Current In the An Glial Cellsmentioning

confidence: 99%

“…The tendency of glial cells to form networks falls off with distance from explants of ORNs and is not contact-dependent, suggesting a diffusible signal acting locally between the glial cells and the ORNs. In other systems, signaling between axons and glial cells can be mediated by transmitters such as glutamate and ATP released from the axons and eliciting an increase in cytosolic calcium in the glial cells (Lohr et al, 2005 andvan den Pol et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

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Development of a glial network in the olfactory nerve: role of calcium and neuronal activity

2010

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In adult olfactory nerves of mammals and moths, a network of glial cells ensheathes small bundles of olfactory receptor axons. In the developing antennal nerve (AN) of the moth Manduca sexta, the axons of olfactory receptor neurons (ORNs) migrate from the olfactory sensory epithelium toward the antennal lobe. Here we explore developmental interactions between ORN axons and AN glial cells. During early stages in AN glial-cell migration, glial cells are highly dye coupled, dividing glia are readily found in the nerve and AN glial cells label strongly for glutamine synthetase. By the end of this period, dye-coupling is rare, glial proliferation has ceased, glutamine synthetase labeling is absent, and glial processes have begun to extend to enwrap bundles of axons, a process that continues throughout the remainder of metamorphic development. Whole-cell and perforated-patch recordings in vivo from AN glia at different stages of network formation revealed two potassium currents and an R-like calcium current. Chronic in vivo exposure to the R-type channel blocker SNX-482 halted or greatly reduced AN glial migration. Chronically blocking spontaneous Na-dependent activity by injection of tetrodotoxin reduced the glial calcium current implicating an activity-dependent interaction between ORNs and glial cells in the development of glial calcium currents.

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Section: Glial Cell Electrophysiology During the Period Of Axon Ingrowthmentioning

confidence: 92%

Section: Role Of Calcium Currents and Effect Of Blocking Neuronal Actmentioning

confidence: 99%

Section: Calcium Current In the An Glial Cellsmentioning

confidence: 99%

Section: Calcium Current In the An Glial Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of a glial network in the olfactory nerve: role of calcium and neuronal activity

2010

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Roles of Ion Transport in Control of Cell Motility

Stock

Ludwig

Hanley

et al. 2013

Comprehensive Physiology

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Cell motility is an essential feature of life. It is essential for reproduction, propagation, embryonic development, and healing processes such as wound closure and a successful immune defense. If out of control, cell motility can become life-threatening as, for example, in metastasis or autoimmune diseases. Regardless of whether ciliary/flagellar or amoeboid movement, controlled motility always requires a concerted action of ion channels and transporters, cytoskeletal elements, and signaling cascades. Ion transport across the plasma membrane contributes to cell motility by affecting the membrane potential and voltage-sensitive ion channels, by inducing local volume changes with the help of aquaporins and by modulating cytosolic Ca(2+) and H(+) concentrations. Voltage-sensitive ion channels serve as voltage detectors in electric fields thus enabling galvanotaxis; local swelling facilitates the outgrowth of protrusions at the leading edge while local shrinkage accompanies the retraction of the cell rear; the cytosolic Ca(2+) concentration exerts its main effect on cytoskeletal dynamics via motor proteins such as myosin or dynein; and both, the intracellular and the extracellular H(+) concentration modulate cell migration and adhesion by tuning the activity of enzymes and signaling molecules in the cytosol as well as the activation state of adhesion molecules at the cell surface. In addition to the actual process of ion transport, both, channels and transporters contribute to cell migration by being part of focal adhesion complexes and/or physically interacting with components of the cytoskeleton. The present article provides an overview of how the numerous ion-transport mechanisms contribute to the various modes of cell motility.

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Expression and function of calcium‐activated potassium channels in human glioma cells

Weaver

Bomben

Sontheimer

2006

Glia

141

151

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Ca(2+)-activated K(+) (K(Ca)) channels are a unique family of ion channels because they are capable of directly communicating calcium signals to changes in cell membrane potential required for cellular processes including but not limited to cellular proliferation and migration. It is now possible to distinguish three families of K(Ca) channels based on differences in their biophysical and pharmacological properties as well as genomic sequence. Using a combination of biochemical, molecular, and biophysical approaches, we show that human tumor cells of astrocytic origin, i.e. glioma cells, express transcripts for all three family members of K(Ca) channels including BK, IK, and all three SK channel types (SK1, SK2, and SK3). The use of selective pharmacological inhibitors shows prominent expression of currents that are inhibited by the BK channel specific inhibitors iberiotoxin and paxilline. However, despite the presence of transcripts for IK and SK, neither clotrimazole, an inhibitor of IK channels, nor apamin, known to block most SK channels inhibited any current. The exclusive expression of functional BK channels was further substantiated by shRNA knockdown experiments, which selectively reduced iberiotoxin sensitive currents. Western blotting of patient biopsies with antibodies specific for all three KCa channel types further substantiated the exclusive expression of BK type KCa channels in vivo. This finding is in sharp contrast to other cancers that express primarily IK channels.

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Blockage of voltage-gated calcium signaling impairs migration of glial cells in vivo

Cited by 41 publications

References 58 publications

Development of a glial network in the olfactory nerve: role of calcium and neuronal activity

Development of a glial network in the olfactory nerve: role of calcium and neuronal activity

Roles of Ion Transport in Control of Cell Motility

Expression and function of calcium‐activated potassium channels in human glioma cells

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