Blockade of Progesterone-Activated Estrous Behavior in Rats by Intracerebral Anisomycin Is Site Specific

Glaser, Jeffrey H.; Barfield, Ronald J.

doi:10.1159/000123914

Cited by 32 publications

(8 citation statements)

References 32 publications

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“…The underlying cellular mechanism that mediates the estradiol‐induced retraction, and progesterone‐induced restoration, of LPDs requires further study. Neurons that express the nuclear estrogen receptor (ER)‐α , and estradiol‐induced progestin receptors are abundant in the VMH (Pfaff and Keiner, 1973; Simerly et al, 1990; DonCarlos et al, 1991) and have well‐documented genotropic effects on mating behavior (Rainbow et al, 1982; Glaser and Barfield, 1984; Meisel and Pfaff, 1984). In addition, estradiol has membrane‐directed effects in this brain region (Kow and Pfaff, 2004).…”

Section: Discussionmentioning

confidence: 99%

Ovarian hormone‐induced reorganization of oxytocin‐labeled dendrites and synapses lateral to the hypothalamic ventromedial nucleus in female rats

Griffin

Ferri

Reyes

et al. 2010

J of Comparative Neurology

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Central oxytocin (OT) modulates many social behaviors, including female rat sexual receptivity, quantified as the copulatory stance known as lordosis. The expression of the lordosis response is modulated by OT action in the ventromedial nucleus of the hypothalamus (VMH), as demonstrated by behavioral pharmacology experiments. However, the subcellular localization of OT in this brain region has been unclear. We tested the hypothesis that ovarian hormones reorganize OT-labeled pre- or postsynaptic elements in the fiber complex lateral to the VMH by using immunoelectron microscopy. OT immunolabeling occurred in axonal boutons identified by the presence of small, clear synaptic vesicles and double labeling with the presynaptic markers synaptophysin and vesicular glutamate transporter 2. OT immunoreactivity also was observed in dendritic profiles, verified with double labeling for the dendrite-specific marker microtubule-associated protein 2. Ovarian hormones did not alter the density of axonal boutons; however, estradiol treatment reduced the density of dendritic profiles by 34%. This effect was reversed when progesterone was given subsequent to estradiol. The effect of estradiol treatment was specific to dendrites that lacked OT immunostaining; the density of OT-labeled dendritic profiles remained constant during estradiol treatment. With the estradiol-induced exit of non-OT-labeled dendritic profiles, the remaining OT-labeled dendritic profiles experienced an increase in their number of synaptic contacts. Thus, hormone treatments that mimic the 4- day rat estrous cycle provoke a chemically coded reorganization of dendrite innervation in the fiber plexus lateral to the VMH that may underlie the hormone-specific effect of OT on reproductive behavior.

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Section: Discussionmentioning

confidence: 99%

Ovarian hormone‐induced reorganization of oxytocin‐labeled dendrites and synapses lateral to the hypothalamic ventromedial nucleus in female rats

Griffin

Ferri

Reyes

et al. 2010

J of Comparative Neurology

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“…Further, data from experiments assessing the effect of protein synthesis inhibitors on progesterone facilitation of sexual behavior are consistent with the hypothesis that PR activation affects neural systems through genomic mechanisms. Treatment of ovariectomized, estrogen-primed rats with anisomycin, delivered into the ven tromedial hypothalamus 30 min before progesterone adminis tration, prevents progesterone-facilitated estrous behavior [9,23]. It is not known whether anisomycin alters the total concen tration of PR compared with other proteins.…”

Section: Discussionmentioning

confidence: 99%

Estrogen plus Progesterone Increases Progestin Receptor Immunoreactivity in the Brain of Ovariectomized Guinea Pigs

Greene

1989

Neuroendocrinology

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The goal of these experiments was to determine the number and distribution of brain cells that contain progestin receptors (PR) and to determine the effect of estrogen and estrogen plus progesterone on PR content of those cells. Ovariectomized adult female guinea pigs were treated with oil (control), or estrogen followed by oil, or estrogen followed by progesterone. As expected, only those animals treated with estrogen plus progesterone became sexually receptive. The cellular content of PR was determined using a monoclonal antibody to the receptor, and standard immunocytochemical techniques. Analysis of the PR-immunoreactive (PR-IR) cells consisted of: (1) mapping the anatomical distribution of PR-IR cells; (2) analyzing the effect of steroid hormones on PR-IR cell number, and (3) determining the effect of steroid hormones on PR immunoreactivity per cell. PR immunoreactivity was located exclusively in the nuclei of cells in the preoptic area and hypothalamus. The most dense collections of PR-IR cells were found in the preoptic area, ventrolateral nucleus of the hypothalamus, and infundibular nucleus. Estrogen caused a dramatic increase in the number of PR-IR cells in these cell groups. Sequential treatment with estrogen plus progesterone further increased PR-IR cell number, in the preoptic area by 65%, in the ventrolateral nucleus by 38%, and in the infundibular nucleus by 49%. A cell-by-cell rating of the PR immunoreactivity was carried out in these three cell groups. We found that the staining intensity across the populations of PR-IR cells was increased by estrogen and further increased by sequential estrogen plus progesterone. Alterations in cellular PR content may contribute importantly to the ability of progesterone target cell groups to perform their specialized roles in steroid-regulated activity.

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“…In mammals, the habenulo-interpeduncular system is involved in limbic functions, including autonomic and endocrine control mechanisms, and aspects of sexual and defensive behavior (Herkenham and Nauta, 1979;Sutherland, 1982;Glaser and Barfield, 1984;Meszaros et al, 1985). However, practically nothing is known about the functions of this system in non-mammals.…”

Section: Functional Considerationsmentioning

confidence: 99%

Afferent and efferent connections of the parapineal organ in lampreys: A tract tracing and immunocytochemical study

1999

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Blockade of Progesterone-Activated Estrous Behavior in Rats by Intracerebral Anisomycin Is Site Specific

Cited by 32 publications

References 32 publications

Ovarian hormone‐induced reorganization of oxytocin‐labeled dendrites and synapses lateral to the hypothalamic ventromedial nucleus in female rats

Ovarian hormone‐induced reorganization of oxytocin‐labeled dendrites and synapses lateral to the hypothalamic ventromedial nucleus in female rats

Estrogen plus Progesterone Increases Progestin Receptor Immunoreactivity in the Brain of Ovariectomized Guinea Pigs

Afferent and efferent connections of the parapineal organ in lampreys: A tract tracing and immunocytochemical study

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