Block copolymer micelles with enzyme molecules at the interfaces

Abstract

This research provides an efficient method for the fabrication of hybrid micelles with enzyme molecules at the interfaces. Amphiphilic block copolymer is synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, and thiol-modified porcine pancreatic lipase (PPL-SH) is obtained by treatment of native PPL with Traut's reagent. PPL-SH is conjugated to the block copolymer chains by thiol-disulfide exchange reaction. In phosphate buffered saline, the bioconjugate self-assembles into micelles with enzyme molecules at the interfaces between hydrophobic cores and hydrophilic coronae. The bioactivity of the enzyme molecules on the micelles are compared with the native enzyme.

A self-crosslinking nanogel scaffold for enhanced catalytic efficiency and stability

“…A typical procedure for the synthesis of thiol-modified lipase is as follows. 53 Native lipase (40 mg) was dissolved in 10 mL of PBS buffer ( pH = 8.0, 0.1 M) in a Schlenk flask. This flask was purged with nitrogen gas flow for more than 30 min.…”

“…54,61 Hybrid nanogel immobilized with lipase In spite of many methods being applied to load proteins into nanogels, covalent conjugation is considered to be one of the most effective approaches to form the linkage between proteins and nanogels. 53,[62][63][64][65][66][67] We then conjugated the lipase onto PEG-b-PNSN-co-PNAG to form a lipase-immobilized hybrid nanogel (nanogels@L) in a one-pot preparation. The thiolmodified lipase is conjugated to the polymer via a thiol-ene reaction and simultaneously undergoes the self-crosslinking process (Scheme 1b).…”

Advances in Insight of Radial Heterogeneous Microstructure and Its Relevance to Mechanical Properties of Polyacrylonitrile‐Based Carbon Fibers

“…The skin-core structure is derived from the "double diffusion" in the production process of PAN fibers, develops during the thermal procession and exists in the product CFs. [17,21,[23][24][25][26] To eliminate the skin-core structure at an early stage, extensive work has been done to study the microstructure of CFs from the preproduction process, PAN fibers production, and oxidative stabilization process. [22,27,28] Recently, plasma preoxidation and microwave carbonization technologies have attracted attention.…”