Block copolymer micelles for drug delivery: Design, characterization and biological significance

Kataoka, Kazunori; Harada, Atsushi; Nagasaki, Yukio

doi:10.1016/j.addr.2012.09.013

Cited by 905 publications

(889 citation statements)

References 76 publications

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“…28-30 Besides the thick PEG shell of micelles, the surface charge of CPT/m determined as ζ-potential at pH 7.4 was found to be close to neutral (+0.26 mV), which is a significant advantage for avoiding the adsorption of charged biomolecules and extending the half-life in the bloodstream. [16][17][18]31 The loading of CPT in the micelles was controlled from 7.50 to 16.3 w/w % (weight of drug/weight of polymer: w/w) ( Table 1), as determined by UV-Vis spectrophotometer. By increasing the [CPT]/[COOH] feed ratio from 1 to 2, the loading of the micelles was practically doubled (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…In this way, combination of PCI with endosomally internalized nanomedicines that specifically release their cargo in response to cytosolic conditions could be an effective approach for augmenting spatiotemporal control of the nanomedicine's functions, and simultaneously evolve into applicable light-activated therapies. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 4 Core-shell block copolymer micelles incorporating therapeutic agents in their core have demonstrated outstanding features as nanomedicines in both preclinical and clinical studies, [16][17][18] including their adaptable drug release in response to specific stimuli by directly engineering the bond between the drugs and the core-forming segment of the block copolymers. [16][17][18] We have previously developed polymeric micelles exploiting disulfide bonds in their core for specifically acting under the reductive conditions of the cytosol, [19][20][21] which shows 100-to 1000-fold higher glutathione (GSH) concentration than those in blood and extracellular milieu (Scheme 1).…”

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confidence: 99%

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Light-Induced Cytosolic Activation of Reduction-Sensitive Camptothecin-Loaded Polymeric Micelles for Spatiotemporally Controlled in Vivo Chemotherapy

Yen

Cabral

et al. 2014

ACS Nano

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Nanomedicines capable of smart operation at the targeted site have the potential to achieve the utmost therapeutic benefits. Providing nanomedicines that respond to endogenous stimuli with an additional external trigger may improve the spatiotemporal control of their functions, while avoiding drawbacks from their inherent tissue distribution. Herein, by exploiting the permeabilization of endosomes induced by photosensitizer agents upon light irradiation, we complemented the intracellular action of polymeric micelles incorporating camptothecin (CPT), which can sharply release the loaded drug in response to the reductive conditions of the cytosol, as an effective strategy for precisely controlling the function of these nanomedicines in vivo, while advancing toward a light-activated chemotherapy. These camptothecin-loaded micelles (CPT/m) were stable in the bloodstream, with minimal drug release in extracellular conditions, leading to prolonged blood circulation and high accumulation in xenografts of rat urothelial carcinoma. With the induction of endosomal permeabilization with the clinically approved photosensitizer, Photofrin, the CPT/m escaped from the endocytic vesicles of cancer cells into the cytosol, as confirmed both in vitro and in vivo by real-time confocal laser microscopies, accelerating the drug release from the micelles only in the irradiated tissues. This spatiotemporal switch significantly enhanced the in vivo antitumor efficacy of CPT/m without eliciting any toxicity, even at a dose 10-fold higher than the maximum tolerated dose of free CPT. Our results indicate the potential of reduction-sensitive drug-loaded polymeric micelles for developing safe chemotherapies after activation by remote triggers, such as light, which are capable of permeabilizing endosomal compartments.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Light-Induced Cytosolic Activation of Reduction-Sensitive Camptothecin-Loaded Polymeric Micelles for Spatiotemporally Controlled in Vivo Chemotherapy

Yen

Cabral

et al. 2014

ACS Nano

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“…Because they can encapsulate guest molecules in their interior, hollow spheres have great potential for applications such as drug storage and delivery (Kataoka et al 2012), separation (Wang et al 1998), adsorbents, microreactors (Vriezema et al 2005), catalysts (Lu et al 2015), supercapacitors (Liu et al 2014), and medical examination and diagnosis (Salata 2004). Methods for the fabrication of hollow spheres have been extensively reported, including template synthesis (Liu and Basu 2009), self-assembly (Breitenkamp and Emrick 2003), emulsion polymerization (Jang and Ha 2002), core removal of dendrimers (Zimmerman et al 2002), and direct polymerization reaction (Kim et al 2010); however, the most commonly used raw materials are synthetic polymers.…”

Section: Introductionmentioning

confidence: 99%

Direct Preparation of Hollow Nanospheres with Kraft Lignin: A Facile Strategy for Effective Utilization of Biomass Waste

Li¹,

Deng²,

Liang³

et al. 2016

BioResources

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This work discusses the preparation and characterization of hollow nanospheres based on kraft lignin (KL). Kraft lignin is a by-product of the papermaking industry and an abundant renewable resource. It was determined that adding water to a KL/THF solution induced KL to form hollow nanospheres via self-assembly. Scanning electron microscopy and transmission electron microscopy confirmed the hollow nanosphere morphology. The shell thickness of the hollow nanospheres was tunable by adjusting the initial KL concentration in THF, making the nanospheres a potential material for the encapsulation and controlled release of guest molecules. Ultraviolet (UV) and Fourier transform infrared (FTIR) spectroscopy confirmed the π-π stacking of aromatic rings as an important and distinctive mechanism for the formation of hollow KL nanospheres. The nanospheres were obtained simply and inexpensively, and they exhibited the characteristics of biocompatibility, biodegradability, and low toxicity. These advantages make hollow KL nanospheres attractive for applications in nanoscience and nanotechnology. This study developed an economically feasible and facile strategy for the effective use of biomass waste in sustainable chemistry.

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“…Further, nanoparticles may be engineered to combine two or more of these therapies, leading to synergistic anticancer efficacy [14][15][16]. Although numerous nanoparticles have been developed as carriers for imaging and therapeutic agents, the majority can be categorized into either pure inorganic [e.g., quantum dots (QDs) [17], metallic nanostructures (gold nanoparticles) [18], and metal oxides (particularly magnetic iron oxides [19], up-conversion nanophosphors [20], and zeolites [21])] or organic materials (e.g., liposomes [22], dendrimers [23], micelles [24], and polymeric hydrogel nanoparticles [25]). Each of these classes of nanoparticles has its own strengths and weaknesses.…”

Section: Introductionmentioning

confidence: 99%

Hybrid Nanoparticles for Cancer Imaging and Therapy

Lin

2015

Cancer Treatment and Research

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Hybrid nanoparticles, composed of both inorganic and organic components, have been exploited as promising platforms for cancer imaging and therapy. This class of nanoparticles can not only retain the beneficial features of both inorganic and organic materials, but also allow systematic fine-tuning of their properties through the judicious combination of functional components. This chapter summarizes recent advances in the design and synthesis of hybrid nanomaterials, with particular emphasis on two main categories of hybrid nanoparticles: Nanoscale metal-organic frameworks (also known as nanoscale coordination polymers) and polysilsesquioxane nanoparticles. Preliminary applications of these hybrid nanoparticles in cancer imaging and therapy are described.

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Block copolymer micelles for drug delivery: Design, characterization and biological significance

Cited by 905 publications

References 76 publications

Light-Induced Cytosolic Activation of Reduction-Sensitive Camptothecin-Loaded Polymeric Micelles for Spatiotemporally Controlled in Vivo Chemotherapy

Light-Induced Cytosolic Activation of Reduction-Sensitive Camptothecin-Loaded Polymeric Micelles for Spatiotemporally Controlled in Vivo Chemotherapy

Direct Preparation of Hollow Nanospheres with Kraft Lignin: A Facile Strategy for Effective Utilization of Biomass Waste

Hybrid Nanoparticles for Cancer Imaging and Therapy

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