2003
DOI: 10.1093/emboj/cdg114
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BLM helicase-dependent transport of p53 to sites of stalled DNA replication forks modulates homologous recombination

Abstract: Diverse functions, including DNA replication, recombination and repair, occur during S phase of the eukaryotic cell cycle. It has been proposed that p53 and BLM help regulate these functions. We show that p53 and BLM accumulated after hydroxyurea (HU) treatment, and physically associated and co-localized with each other and with RAD51 at sites of stalled DNA replication forks. HU-induced relocalization of BLM to RAD51 foci was p53 independent. However, BLM was required for efficient localization of either wild… Show more

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Cited by 191 publications
(225 citation statements)
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“…These experiments were reproduced in independently derived cell lines (data not shown). Immunofluorescence analysis of [pS 15 ]-p53 demonstrated its nuclear localization in Ba/F3-EPOR and Ba/F3-EPOR-JAK2 wt that was strongly inhibited in Ba/F3-EPOR-JAK2 V617F cells (Sengupta et al, 2003) (Figure 1d). Thus, JAK2 V617F expression appeared to reduce p53 stabilization and phosphorylation upon DNA damage.…”
Section: Jak2mentioning
confidence: 99%
“…These experiments were reproduced in independently derived cell lines (data not shown). Immunofluorescence analysis of [pS 15 ]-p53 demonstrated its nuclear localization in Ba/F3-EPOR and Ba/F3-EPOR-JAK2 wt that was strongly inhibited in Ba/F3-EPOR-JAK2 V617F cells (Sengupta et al, 2003) (Figure 1d). Thus, JAK2 V617F expression appeared to reduce p53 stabilization and phosphorylation upon DNA damage.…”
Section: Jak2mentioning
confidence: 99%
“…It has been reported that p53 associates with several proteins including BLM, BRCA1, BRCA2, Rad52 and RPA (Yamaguchi-Iwai Marmorstein et al, 1998;Zhang et al, 1998;Linke et al, 2003;Sengupta et al, 2003) and inhibits the Rad51-dependent HR (Cromie et al, 2001;Linke et al, 2003). When DSBs were induced, the association of p53 and Rad51 was prevented by an unknown mechanism.…”
Section: Vpr Induces Dsbs and Enhances Hrmentioning
confidence: 99%
“…p53 was shown to respond to the replication block induced by aphidicolin (Gottifredi et al, 2001;Nayak and Das, 2002) and colocalized with BLM, Rad51 and 53BP1 at the sites of stalled DNA replication fork (Sengupta et al, 2003(Sengupta et al, , 2004. p53 also accumulated during S-phase in Cdc7À/À ES cells with defective replication origin firing (Kim et al, 2002).…”
Section: Introductionmentioning
confidence: 96%