Blind-deconvolution optical-resolution photoacoustic microscopy in vivo

Chen, Jianhua; Lin, Riqiang; Huina, Wang; Meng, Jing; Zheng, Hairong; Liu, Song

doi:10.1364/oe.21.007316

Cited by 90 publications

(81 citation statements)

References 38 publications

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“…We find that the two numbers are similar and thus, using the criterion of AMSE is sufficient. Similar observations of this consistency were reported in [33]. The 1D lateral profiles from the original ARPAM and D-ARPAM are shown in Fig.…”

Section: Lateral Resolution Of D-arpamsupporting

confidence: 89%

“…Deconvolution approaches have recently been investigated in PA imaging in ORPAM and PA computed tomography systems [18,[30][31][32][33][34]. Although ARPAM has been demonstrated as a valuable tool for biomedical imaging, there is no study, to our knowledge, on the application of deconvolution methods in scanning-based ARPAM to improve the image quality.…”

Section: Introductionmentioning

confidence: 99%

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In vivo deconvolution acoustic-resolution photoacoustic microscopy in three dimensions

Cai¹,

Li²,

Chen³

2016

Biomed. Opt. Express

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Acoustic-resolution photoacoustic microscopy (ARPAM) provides a spatial resolution on the order of tens of micrometers, and is becoming an essential tool for imaging fine structures, such as the subcutaneous microvasculature. High lateral resolution of ARPAM is achieved using high numerical aperture (NA) of acoustic transducer; however, the depth of focus and working distance will be deteriorated correspondingly, thus sacrificing the imaging range and accessible depth. The axial resolution of ARPAM is limited by the transducer's bandwidth. In this work, we develop deconvolution ARPAM (D-ARPAM) in three dimensions that can improve the lateral resolution by 1.8 and 3.7 times and the axial resolution by 1.7 and 2.7 times, depending on the adopted criteria, using a 20-MHz focused transducer without physically increasing its NA and bandwidth. The resolution enhancement in three dimensions by D-ARPAM is also demonstrated by in vivo imaging of the microvasculature of a chick embryo. The proposed D-ARPAM has potential for biomedical imaging that simultaneously requires high spatial resolution, extended imaging range, and long accessible depth.

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Section: Lateral Resolution Of D-arpamsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

In vivo deconvolution acoustic-resolution photoacoustic microscopy in three dimensions

Cai¹,

Li²,

Chen³

2016

Biomed. Opt. Express

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“…1). Further details of the OR-PAM system can be found in our previous publication [21]. For in vivo imaging, an anesthetized female BALB/c mouse (around six-week old and weighing ~20 g) was used.…”

Section: Or-pam System and Image Acquisitionmentioning

confidence: 99%

Multi-parametric quantitative microvascular imaging with optical-resolution photoacoustic microscopy in vivo

Yang¹,

Chen²,

Yao³

et al. 2014

Opt. Express

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Many diseases involve either the formation of new blood vessels (e.g., tumor angiogenesis) or the damage of existing ones (e.g., diabetic retinopathy) at the microcirculation level. Optical-resolution photoacoustic microscopy (OR-PAM), capable of imaging microvessels in 3D in vivo down to individual capillaries using endogenous contrast, has the potential to reveal microvascular information critical to the diagnosis and staging of microcirculation-related diseases. In this study, we have developed a dedicated microvascular quantification (MQ) algorithm for OR-PAM to automatically quantify multiple microvascular morphological parameters in parallel, including the vessel diameter distribution, the microvessel density, the vascular tortuosity, and the fractal dimension. The algorithm has been tested on in vivo OR-PAM images of a healthy mouse, demonstrating high accuracy for microvascular segmentation and quantification. The developed MQ algorithm for OR-PAM may greatly facilitate quantitative imaging of tumor angiogenesis and many other microcirculation related diseases in vivo. M. Arbeit, "VEGF is essential for hypoxia-inducible factor-mediated neovascularization but dispensable for endothelial sprouting," Proc. Natl. Acad. Sci. U. S. A. 108(32), 13264-13269 (2011). 9. J. Folkman, "Angiogenesis in cancer, vascular, rheumatoid and other disease," Nat. Med. 1(1), 27-30 (1995 1182-1186 (1971). 11. J. W. Baish and R. K. Jain, "Cancer, angiogenesis and fractals," Nat. Med. 4(9), 984 (1998). 12. R. K. Jain, "Normalizing tumor vasculature with anti-angiogenic therapy: A new paradigm for combination therapy," Nat. Med. 7(9), 987-989 (2001)

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“…As shown in Fig. 1(b), according to the KV model, the tissue-speci¯c phase lag between the incident laser and the produced PA signal can be expressed as 49 ¼…”

Section: Methods Of the Pave Imagingmentioning

confidence: 99%

Photoacoustic viscoelasticity imaging dedicated to mechanical characterization of biological tissues

Shi

Yang

Wang

2017

J. Innov. Opt. Health Sci.

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Since changes in mechanical properties of biological tissues are often closely related to pathology, the viscoelastic properties are important physical parameters for medical diagnosis. A photoacoustic (PA) phase-resolved method for noninvasively characterizing the biological tissue viscoelasticity has been proposed by Gao et al. [G. Gao, S. Yang, D. Xing, \Viscoelasticity imaging of biological tissues with phase-resolved photoacoustic measurement," Opt. Lett. 36, 3341-3343 (2011)]. The mathematical relationship between the PA phase delay and the viscosity-elasticity ratio has been theoretically deduced. Moreover, systems of PA viscoelasticity (PAVE) imaging including PAVE microscopy and PAVE endoscopy were developed, and high-PA-phase contrast images re°ecting the tissue viscoelasticity information have been successfully achieved. The PAVE method has been developed in tumor detection, atherosclerosis characterization and related vascular endoscopy. We reviewed the development of the PAVE technique and its applications in biomedical¯elds. It is believed that PAVE imaging is of great potential in both biomedical applications and clinical studies.

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Blind-deconvolution optical-resolution photoacoustic microscopy in vivo

Cited by 90 publications

References 38 publications

In vivo deconvolution acoustic-resolution photoacoustic microscopy in three dimensions

In vivo deconvolution acoustic-resolution photoacoustic microscopy in three dimensions

Multi-parametric quantitative microvascular imaging with optical-resolution photoacoustic microscopy in vivo

Photoacoustic viscoelasticity imaging dedicated to mechanical characterization of biological tissues

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