Bletilla striata oligosaccharides improve metabolic syndrome through modulation of gut microbiota and intestinal metabolites in high fat diet-fed mice

Hu, Baifei; Cheng, Ya; Leung, Elaine Lai‐Han; Zhu, Lin; Hu, Haiming; Zhang, Zhigang; Zheng, Junping; Liu, Hongtao

doi:10.1016/j.phrs.2020.104942

Cited by 39 publications

(27 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We consistently found that E. coli were more existed in the liver and spleen of mice in the ETEC group than that in the HNatreated group, suggesting that HNa-treated mice can defend against the intestinal E. coli translocation by decreasing intestinal permeability. Consistent with our results, an earlier study showed that dietary HNa supplementation decreased the E. coli adhesion and colonization in the gut of ETEC infected piglets, indicating the underlying inhibitory effects of HNa on pathogen invasion (Hu et al, 2020). Taken together, HNa administration had protective effects on intestinal barrier integrity of the ETEC K88 infected mice via increasing TJ proteins and mucins expression, promoting enterocyte proliferation, and reducing the release of inflammatory cytokines.…”

Section: Discussionsupporting

confidence: 92%

“…Fecal samples were prepared according to previously described procedures (Hu et al, 2020). LC-MS/MS analyses were performed using an UHPLC (1290 Infinity LC, Agilent Technologies, Palo Alto, CA, United States) coupled to a quadrupole time-of-flight (TripleTOF 6600, AB Sciex, United States) in Shanghai Applied Protein Technology Co., Ltd. For HILIC separation, samples were analyzed using a 2.1 mm × 100 mm ACQUIY UPLC BEH Amide 1.7 µm column (Waters, Milford, MA, United States).…”

Section: Fecal Metabolites Assessmentmentioning

confidence: 99%

See 1 more Smart Citation

Sodium Humate Alleviates Enterotoxigenic Escherichia coli-Induced Intestinal Dysfunction via Alteration of Intestinal Microbiota and Metabolites in Mice

Wang

Liu

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Enterotoxigenic Escherichia coli (ETEC) can damage intestinal epithelial barrier function and lead to serious intestinal diarrhea in newborns and young animals. Sodium humate (HNa) is natural organic bioactive compound possessing antibacterial, anti-inflammatory, and anti-diarrheal properties. This study investigated the alleviative potential of HNa on the impaired intestinal barrier and intestinal inflammation, and regulatory effects on gut microbiota and metabolites in ETEC K88 infected mice. A total of 30 female mice were randomly assigned into three groups. The mice in the control and ETEC groups were gavaged with 0.2 mL of sterile saline, while the mice in the ETEC + HNa group were gavaged with 0.2 mL of 5% HNa, daily. On day 8, the mice in ETEC and ETEC + HNa group were challenged with ETEC K88. The trial lasted for 12 days. HNa administration elevated ETEC K88-induced body weight loss and ameliorated jejunum and colon pathological injury. HNa also reduced the levels of pro-inflammatory cytokines in the serum, jejunum, and colon. Additionally, HNa reduced intestinal barrier damage by up-regulating the expression of tight junction proteins (TJPs) and mucosal repair factors. 16s rDNA gene sequencing results showed that HNa increased the abundance of beneficial bacteria Lactobacillus, Prevotella_9, and Odoribacter but decreased the abundance of pathogenic bacteria Escherichia and Gastranaerophilales in the feces of mice. Moreover, metabolomic analysis revealed that the concentrations of 15 metabolites, the pathways of protein digestion and absorption, and propanoic acid metabolism were changed by HNa administration. In conclusion, HNa could alleviate ETEC K88-induced intestinal dysfunction through restoring intestinal barrier integrity, modulating gut microbiota, and metabolites.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Fecal Metabolites Assessmentmentioning

confidence: 99%

Sodium Humate Alleviates Enterotoxigenic Escherichia coli-Induced Intestinal Dysfunction via Alteration of Intestinal Microbiota and Metabolites in Mice

Wang

Liu

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…However, after BO treatment, the severity of bloody stools was lessened with the reinstated colon injury and reduced spleen weight in UC mice, indicating the preventive effect of BO on the occurrence of UC ( Figures 1A–E ). Moreover, BO effectively suppressed DSS-induced damage to the intestinal tract in UC mice ( Figures 1F–H ), parallel to our previous studies, in which BO were found to avoid the destruction of gut barrier in obese mice by the regulation of gut microbiota ( Hu et al, 2020 ).…”

Section: Discussionsupporting

confidence: 88%

“…B. striata oligosaccharides (BO) were extracted from B. striata ( Supplementary Methods ; Supplementary Figures S1, S2 ). The preparation methods were carried out according to the previous report, and the molecular weight of BO was 720–1080 Da ( Chen et al, 2019 ; Hu et al, 2020 ). Bletilla striata (Thunb.)…”

Section: Methodsmentioning

confidence: 99%

Bletilla striata Oligosaccharides Improve Ulcerative Colitis by Regulating Gut Microbiota and Intestinal Metabolites in Dextran Sulfate Sodium-Induced Mice

Zhu

Cheng

et al. 2022

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

This study aimed to elucidate the mechanism of Bletilla striata oligosaccharides (BO) in the treatment of ulcerative colitis (UC). A UC mouse model was induced by 3% Dextran sodium sulfate (DSS), and BO (200 mg/kg/d) were administered for intervention. The results show that BO effectively inhibited the release of intestinal inflammatory cytokines such as IL-6, TNF-α, and IL-1β. Also, BO profoundly elevated the secretion of mucins and the expression of tight junction (TJ) proteins to attenuate dysfunction of the intestinal barrier. The 16S rDNA sequencing and liquid chromatography/gas chromatography-mass spectrometer (LC/GC-MS) analysis of mouse feces revealed that BO regulated the disturbance of gut microbiota and intestinal metabolites. By using the in vitro fermentation broth of BO and gut microbiota-depleted mice treated with antibiotics, we confirmed the protection of BO against UC. In conclusion, BO played a role in improving UC by modulating gut microbial composition and intestinal metabolites, which provided new therapeutic strategies for UC treatment.

show abstract

“…As a bridge between diet and host health, gut microbiota not only affects the digestion and absorption of nutrients in the diet, but also regulates the normal physiological functions and the occurrence of diseases in the host [ 3 ]. Recently, with the help of next-generation high-throughput sequencing technology, bioinformatics and metagenomics [ 4 ], researchers have verified that gut microbiota is vital to host health, and the disruption of gut microbiota has been shown to be associated with multiple diseases, including metabolic syndrome [ 5 ], obesity [ 6 ], tumor [ 7 ], diabetes [ 8 ], HIV [ 9 ], flu [ 10 ], fatigue [ 11 ], brain health [ 12 ], etc. At the same time, intestinal flora transplantation has shown promising application prospects in the treatment of diseases [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of an Antibacterial Polysaccharide Produced by Chaetomium globosum CGMCC 6882 on the Gut Microbiota of Mice

Sun

Wang

et al. 2021

Foods

View full text Add to dashboard Cite

Previously, a polysaccharide produced by Chaetomium globosum CGMCC 6882 was found to have antibacterial activity, but its toxic effects on body health and gut microbiota were concealed. Recent results showed that this polysaccharide was safe to Caco-2 cells and mice, while it reduced the body weight gain of mice from 10.5 ± 1.21 g to 8.4 ± 1.17 g after 28 days administration. Acetate, propionate, butyrate and total short-chain fatty acids concentrations increased from 23.85 ± 1.37 μmol/g, 10.23 ± 0.78 μmol/g, 7.15 ± 0.35 μmol/g and 41.23 ± 0.86 μmol/g to 42.77 ± 1.29 μmol/g, 20.03 ± 1.44 μmol/g, 12.06 ± 0.51 μmol/g and 74.86 ± 2.07 μmol/g, respectively. Furthermore, this polysaccharide enriched the abundance of gut microbiota and the Firmicutes/Bacteroidetes ratio was increased from 0.5172 to 0.7238. Overall, this study provides good guidance for the promising application of polysaccharides as preservatives in foods and in other fields in the future.

show abstract

Bletilla striata oligosaccharides improve metabolic syndrome through modulation of gut microbiota and intestinal metabolites in high fat diet-fed mice

Cited by 39 publications

References 48 publications

Sodium Humate Alleviates Enterotoxigenic Escherichia coli-Induced Intestinal Dysfunction via Alteration of Intestinal Microbiota and Metabolites in Mice

Sodium Humate Alleviates Enterotoxigenic Escherichia coli-Induced Intestinal Dysfunction via Alteration of Intestinal Microbiota and Metabolites in Mice

Bletilla striata Oligosaccharides Improve Ulcerative Colitis by Regulating Gut Microbiota and Intestinal Metabolites in Dextran Sulfate Sodium-Induced Mice

Effect of an Antibacterial Polysaccharide Produced by Chaetomium globosum CGMCC 6882 on the Gut Microbiota of Mice

Contact Info

Product

Resources

About