Bleomycin delivery into cancer cells<i>in vitro</i>with ultrasound and SonoVue® or BR14® microbubbles

Lamanauskas, Nerijus; Novell, Anthony; Escoffre, Jean‐Michel; Venslauskas, Mindaugas Saulius; Šatkauskas, Saulius; Bouakaz, Ayache

doi:10.3109/1061186x.2012.761223

Cited by 37 publications

(30 citation statements)

References 42 publications

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“…[13][14][15] The acoustic parameters used for sonoporation showing increased cellular uptake of chemotherapeutics and genes vary from low-intensity diagnostic ultrasound [mechanical index (MI) < 0.3] (Refs. [16][17][18][19][20][21][22][23][24][25][26][27][28][29] to high-intensity diagnostic ultrasound (MI > 1.0). 9,[30][31][32][33][34] Throughout literature, the acoustic settings used to induce sonoporation vary drastically, with a broad range of these settings showing improved drug and gene delivery.…”

Section: Purposementioning

confidence: 99%

Treatment of human pancreatic cancer using combined ultrasound, microbubbles, and gemcitabine: A clinical case study

et al. 2013

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Section: Purposementioning

confidence: 99%

Treatment of human pancreatic cancer using combined ultrasound, microbubbles, and gemcitabine: A clinical case study

et al. 2013

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“…This is especially useful for cancer therapy as microbubbles loaded with a chemotherapeutic agent can respond to an ultrasonic field at a localized site with minimum effect on healthy tissues. On an alternative spectrum, more intense ultrasound inducing cavitational bubbles has been shown to play a role in cell disruption of microbial [19] and tumor cells [20–22]. Examples of cell disruption include the ability to effectively lyse cells, releasing intracellular contents for lab‐on‐a‐chip diagnostics without denaturation of biomarker proteins [19].…”

Section: Discussionmentioning

confidence: 99%

Microbubble‐mediated sonoporation for highly efficient transfection of recalcitrant human B‐ cell lines

Yong

Tandiono

et al. 2014

Biotechnology Journal

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Sonoporation has not been widely explored as a strategy for the transfection of heterologous genes into notoriously difficult-to-transfect mammalian cell lines such as B cells. This technology utilizes ultrasound to create transient pores in the cell membrane, thus allowing the uptake of extraneous DNA into eukaryotic and prokaryotic cells, which is further enhanced by cationic microbubbles. This study investigates the use of sonoporation to deliver a plasmid encoding green fluorescent protein (GFP) into three human B-cell lines (Ramos, Raji, Daudi). A higher transfection efficiency (TE) of >42% was achieved using sonoporation compared with <3% TE using the conventional lipofectamine method for Ramos cells. Upon further antibiotic selection of the transfected population for two weeks, we successfully enriched a stable population of GFP-positive Ramos cells (>70%). Using the same strategy, Raji and Daudi B cells were also successfully transfected and enriched to 67 and 99% GFP-positive cells, respectively. Here, we present sonoporation as a feasible non-viral strategy for stable and highly efficient heterologous transfection of recalcitrant B-cell lines. This is the first demonstration of a non-viral method yielding transfection efficiencies significantly higher (42%) than the best reported values of electroporation (30%) for Ramos B-cell lines.

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“…These include studies, for example, that use ultrasound contrast agents to enhance acoustic cavitation for this purpose [35]. These studies, typically carried out in a monolayer of cells, in open culture wells, typify the lack of suitability of these experimental setups for representing in vivo conditions.…”

Section: Introductionmentioning

confidence: 99%

In vitro methods for evaluating therapeutic ultrasound exposures: present-day models and future innovations

2013

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Although preclinical experiments are ultimately required to evaluate new therapeutic ultrasound exposures and devices prior to clinical trials, in vitro experiments can play an important role in the developmental process. A variety of in vitro methods have been developed, where each of these has demonstrated their utility for various test purposes. These include inert tissue-mimicking phantoms, which can incorporate thermocouples or cells and ex vivo tissue. Cell-based methods have also been used, both in monolayer and suspension. More biologically relevant platforms have also shown utility, such as blood clots and collagen gels. Each of these methods possesses characteristics that are well suited for various well-defined investigative goals. None, however, incorporate all the properties of real tissues, which include a 3D environment and live cells that may be maintained long-term post-treatment. This review is intended to provide an overview of the existing application-specific in vitro methods available to therapeutic ultrasound investigators, highlighting their advantages and limitations. Additional reporting is presented on the exciting and emerging field of 3D biological scaffolds, employing methods and materials adapted from tissue engineering. This type of platform holds much promise for achieving more representative conditions of those found in vivo, especially important for the newest sphere of therapeutic applications, based on molecular changes that may be generated in response to non-destructive exposures.

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Bleomycin delivery into cancer cellsin vitrowith ultrasound and SonoVue® or BR14® microbubbles

Abstract: Sonoporation is a feasible method to deliver BLM in to several types of human cancer cell lines. Efficiency of cell sonoporation correlated well with MB sonodestruction, providing a possibility to optimize US parameters by measuring MB sonodestruction.

Cited by 37 publications

References 42 publications

Treatment of human pancreatic cancer using combined ultrasound, microbubbles, and gemcitabine: A clinical case study

Treatment of human pancreatic cancer using combined ultrasound, microbubbles, and gemcitabine: A clinical case study

Microbubble‐mediated sonoporation for highly efficient transfection of recalcitrant human B‐ cell lines

In vitro methods for evaluating therapeutic ultrasound exposures: present-day models and future innovations

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