Blend electrospinning, coaxial electrospinning, and emulsion electrospinning techniques

Buzgo, Matěj; Míčková, Andrea; Rampichová, Michala; Doupnik, Miroslav

doi:10.1016/b978-0-08-102198-9.00011-9

Cited by 64 publications

(77 citation statements)

References 180 publications

(180 reference statements)

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“…An initial burst release followed by a sustained release period observed for all scaffolds to reach a constant rate. In general, the mechanism of protein release from biodegradable polymers is affected by the diffusion through the pores and/or the polymer degradation . In core–shell fibrous scaffolds, a reservoir/barrier mechanism also determines the protein release kinetics through their diffusion across the shell barrier .…”

Section: Discussionmentioning

confidence: 99%

“…In general, the mechanism of protein release from biodegradable polymers is affected by the diffusion through the pores and/or the polymer degradation. 64 In core-shell fibrous scaffolds, a reservoir/barrier mechanism also determines the protein release kinetics through their diffusion across the shell barrier. 65 As observed in our degradation profile study, the diffusion mechanism was a predominant mechanism for protein release from PCL scaffolds with very slow degradation.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

Ghasemkhah

Latifi

Hadjizadeh

et al. 2019

J Biomedical Materials Res

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The biomaterials design as core–shell structures opens a new door to the release of susceptible biomolecules in a controllable manner and enables to place natural biomaterials as shell layers to impart the effective biofunctional features at surfaces. In this study, core–shell designed scaffolds were prepared using coaxial electrospinning with hybrid of gelatin (GT)/polycaprolactone (PCL) at different weight ratios as their shell and protein solution as their core, followed by cross‐linking to impart controllable release rates, tunable mechanical properties, and enhanced cytocompatibility. SEM, FM, and TEM confirmed the successful production of uniform core–shell nanofibers and homogeneous protein distribution. Results showed that an increase in GT proportion in the shell resulted in a decrease in fiber diameter, an increase of Young's modulus, and an intense burst release of BSA 0.2% which could be controlled through cross‐linking. The mechanical tests revealed that the GT/PCL combining and cross‐linking improved mechanical properties which correlated with an increase in spreading and proliferation of HUVECs. A slight burst release was also detected from BSA 0.05% and EGF encapsulated GT73P‐cross‐linked scaffold which demonstrated their applicability for a controlled release of dilute proteins. We were able to successfully incorporate two types of protein with different concentrations without supporting polymer into the GT shell to provide scaffolds possessing tunable mechanical properties and controllable release rates through blending with PCL at different ratios and/or cross‐linking. These findings are promising to promote delivery systems of angiogenic growth factors that are needed a sustained release with different rates at each angiogenesis stage. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

Ghasemkhah

Latifi

Hadjizadeh

et al. 2019

J Biomedical Materials Res

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“…The production rate of a conventional electrospinning system is less than 10 g h −1 of nanofibers, depending on polymer concentration and operating conditions [49]. Thus, various researches have been devoted to the design of innovative and versatile electrospinning configurations capable of fabricating complex constructs more easily [50]. In fact, there are already several electrospinning systems available that include blend (or co-), side-by-side, multi-jet, co-axial and emulsion electrospinning ( Figure 2) [41].…”

Section: Electrospinning In the Production Of Nanofibrous Scaffoldsmentioning

confidence: 99%

“…A great number of limitations from the previous methods can be overcome using emulsion electrospinning [50]. This method allows for two immiscible solutions to be electrospun into a single fibrous scaffold and, therefore, maximize the potential for TE and drug delivery applications [63,64].…”

Section: Composition Natural (Nt)mentioning

confidence: 99%

Poly(Vinyl Alcohol)-Based Nanofibrous Electrospun Scaffolds for Tissue Engineering Applications

2019

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Tissue engineering (TE) holds an enormous potential to develop functional scaffolds resembling the structural organization of native tissues, to improve or replace biological functions and prevent organ transplantation. Amongst the many scaffolding techniques, electrospinning has gained widespread interest because of its outstanding features that enable the production of non-woven fibrous structures with a dimensional organization similar to the extracellular matrix. Various polymers can be electrospun in the form of three-dimensional scaffolds. However, very few are successfully processed using environmentally friendly solvents; poly(vinyl alcohol) (PVA) is one of those. PVA has been investigated for TE scaffolding production due to its excellent biocompatibility, biodegradability, chemo-thermal stability, mechanical performance and, most importantly, because of its ability to be dissolved in aqueous solutions. Here, a complete overview of the applications and recent advances in PVA-based electrospun nanofibrous scaffolds fabrication is provided. The most important achievements in bone, cartilage, skin, vascular, neural and corneal biomedicine, using PVA as a base substrate, are highlighted. Additionally, general concepts concerning the electrospinning technique, the stability of PVA when processed, and crosslinking alternatives to glutaraldehyde are as well reviewed.

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“…For achieving perfect encapsulation, polymers should ideally be chosen for perfect compatibility/miscibility with the hydrophilic or lipophilic drugs. However, recent studies have shown that coaxial electrospinning allows also the manufacturing of core-shell nanofibers with homogeneously dispersed drugs of drug/polymer combinations with low compatibility/miscibility [45,46,[53][54][55][56][57]. Another big advantage of this technique is that the shell provides protection to the core-loaded drugs through shielding it against 2-Acetyl-pyridine thiosemicarbazones have been studied since the 1960s for their interesting biological activities, and they stood also at the cradle when TSC were first investigated against herpes simplex viral infections [18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Release Behavior of a Thiosemicarbazone from Electrospun Polyvinyl Alcohol Core-Shell Nanofibers

et al. 2020

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Mats of polyvinyl alcohol (PVA) core–shell nanofibers were produced using coaxial electrospinning in the presence of a thiosemicarbazone (TSC) N4-(S)-1-phenylethyl)-2-(pyridin-2-yl-ethylidene)hydrazine-1-carbothioamide (HapyTSCmB). Monolithic fibers with 0% or 5% TSC and core–shell fibers with 10% TSC in the spinning solution were studied to compare stability and release rates. SEM showed the formation of uniform, bead-free, cylindrical, and smooth fibers. NMR spectroscopy and thermal analysis (TG/DTA) gave proof for the chemical integrity of the TSC in the fiber mats after the electrospinning process. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy showed no TSC on the surface of the PVA/TSC-PVA fibers confirming the core–shell character. The TSC release profiles of the fibers as studied using UV-vis absorption spectroscopy showed a slower release from the PVA/TSC-PVA core–shell structure compared with the monolithic PVA/TSC fibers as well as lower cumulative release percentage (17%). Out of several release models, the Korsmeyer–Peppas model gave the best fit to the experimental data. The main release phase can be described with a Fick-type diffusion mechanism. Antibacterial properties were tested against the Gram-positive Staphylococcus aureus bacterium and gave a minimal inhibitory concentration of 12.5 μg/mL. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT)-based cytotoxicity experiments showed that the cell viability of fibroblast at different contents of TSC was slightly decreased from 1.5% up to 3.5% when compared to control cells.

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Blend electrospinning, coaxial electrospinning, and emulsion electrospinning techniques

Cited by 64 publications

References 180 publications

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

Poly(Vinyl Alcohol)-Based Nanofibrous Electrospun Scaffolds for Tissue Engineering Applications

Characterization and Release Behavior of a Thiosemicarbazone from Electrospun Polyvinyl Alcohol Core-Shell Nanofibers

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