Bleach boosting effect of xylanase A from Bacillus halodurans C-125 in ECF bleaching of wheat straw pulp

Lin, Xiaoqiong; Han, Sang‐Ik; Zhang, Na; Hu, Hui; Zheng, Suiping; Ye, Yanrui; Lin, Ying

doi:10.1016/j.enzmictec.2012.10.011

Cited by 51 publications

(29 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The extremophilic communities are physiologically important, since they have potential applications in medicine, including antibiotics, local application in skin care products, stable enzymes in biosensors, and drug excipients (Irwin 2010). Thermophiles, as extremophiles, are a good source of thermostable enzymes and have been exploited for pharmaceutical and many other industrial applications (Lin et al 2013;Oliveira et al 2013;Panda et al 2013;Park et al 2013). It is known that microbial adaptation is regulated by changes in cellular enzymes, membrane lipids, and their morphology, leading to a variation in functional dynamics and plasticity through redox and signaling systems of the organism (Contera et al 2010;Pocock et al 2011;Diakogiannis et al 2013).…”

Section: Introductionmentioning

confidence: 99%

A comparative study of fatty acid profile and formation of biofilm inGeobacillus gargensisexposed to variable abiotic stress

et al. 2015

View full text Add to dashboard Cite

Understanding bacterial fatty acid (FA) profile has a great taxonomic significance as well as clinical importance for diagnosis issues. Both the composition and nature of membrane FAs change under different nutritional, biotic and (or) abiotic stresses, and environmental stress. Bacteria produce both odd-carbon as well as branched-chain fatty acids (BCFAs). This study was designed to examine the effect of abiotic pressure, including salinity, temperature, pH, and oxinic stress on the growth, development, and FA profile in thermophilic Geobacillus gargensis. Under these stresses, 3 parametric ratios, 2-methyl fatty acids/3-methyl fatty acids (iso-/anteiso-FAs), BCFAs/straight-chain saturated fatty acids (SCSFA), and SCSFAs/straight-chain unsaturated fatty acids (SCUFA), in addition to total lipids affected by variable stresses were measured. Our results indicate that the ratio of total iso-/anteiso-FAs increased at the acidic pH range of 4.1-5.2 and decreased with increasing pH. The reverse was true for salt stress when iso-/anteiso-FAs ratio increased with salt concentration. The BCFAs/SCSFAs and SCSFAs/SCUFAs ratios increased at neutral and alkaline pH and high salt concentration, reduced incubation time, and comparatively high temperature (55-65 °C) of the growth medium. The bacterial total lipid percentage deceased with increasing salt concentration, incubation period, but it increased with temperature. The formation of extracellular polymeric substances was observed under all stress conditions and with the addition of sodium dodecyl sulfate (2 and 5 mmol/L) to the growth medium. The membrane phospholipid composition of the bacterium was analyzed by thin-layer chromatography.

show abstract

Section: Introductionmentioning

confidence: 99%

A comparative study of fatty acid profile and formation of biofilm inGeobacillus gargensisexposed to variable abiotic stress

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Lin et al (2013) reported 4.65, 9.3, and 13.95% increase in bursting index after treatment of wheat straw SAQ pulp with pulpzyme HC, recombinant xylanase from B. halodurans and commercial xylanase AU-PE89, respectively. Biobleaching of eucalyptus kraft pulp with xylanase producing B. halodurans FNP 135 culture and produced by SmF and SSF resulted in 8.6 and 3.3% increase in viscosity, 20.7 and 17.5% increase in tear factor, 13.7 and 12% increase in burst factor, and 8.7 and 6.7% increase in breaking length, respectively (Sharma et al 2015).…”

Section: Resultsmentioning

confidence: 99%

Improved efficacy of ultrafiltered xylanase–pectinase concoction in biobleaching of plywood waste soda pulp

Sharma

Agrawal

Yadav³

et al. 2017

3 Biotech

View full text Add to dashboard Cite

The effect of ultrafiltered xylanase–pectinase concoction produced simultaneously by a bacterial isolate using agro-waste-based media was assessed in prebleaching of plywood waste pulp. Ultrafiltered enzymes caused 12.5% reduction in kappa number at reduced enzyme dose of xylanase–pectinase (4.0–0.8 IU) per gram of pulp under optimized conditions at pH 8.5, temperature 55 °C, and treatment period of 2 h. Using this methodology, amount of Cl2–ClO2 consumption can be reduced up to 30 and 28.86%. Significant improvement in physical and optical properties of pulp was obtained along with an additional reduction in BOD and COD values up to 18.13 and 21.66% using this novel biodelignification approach. This is the first report showing the advantages of using ultrafiltered xylanase–pectinase over crude enzymes in enhancing the bleaching capacity of pulp. This study focussing on the development of good quality paper with less pollution generating strategy will definitely prove a boon for industries.

show abstract

“…Many xylanases have been isolated, cloned and expressed from microorganisms such as bacteria, fungi, and yeasts (Banka, Guralp, & Gulari, ; Huang, Wang, & Xiao, ; Jeya, Thiagarajan, Lee, & Gunasekaran, ; Lee et al, ; Lin et al, ). The present study achieved cloning and secretive expression of a xylanase gene from B. subtilis E20 named BsXynE20 .…”

Section: Discussionmentioning

confidence: 99%

Secretory expression and characterization of xylanase isolated from Bacillus subtilis E20 increase the utilization of plant ingredients in tilapia feed

Tsai

Wang

et al. 2019

Aquac Res

View full text Add to dashboard Cite

Bacillus subtilis E20, an isolate from natto – a Japanese fermented soy product – is used in a wide variety of marine and freshwater aquatic species to enhance growth and immunity. This paper describes the cloning, expression and characterization of xylanase isolated from B. subtilis E20 in B. subtilis RIK1285. The BsXynE20 gene was identified to encode a 213‐amino‐acid protein for a glycoside hydrolase family 11 xylanase containing a 28‐residue signal peptide whose cleavage yields a 185‐residue mature protein with a predicted molecular weight of 20.25 kDa. The full length of the BsXynE20 gene was subcloned into the pBE‐S expression vector for secretory production of recombinant BsXynE20 in B. subtilis RIK1285. Western blot and zymogram analysis, respectively, revealed that recombinant BsXynE20 can be secreted into culture medium and is a functional xylanase. The xylanase exhibited optimal activity at pH 5.0–6.0 and 60°C. Using recombinant BsXynE20‐pretreated duckweed meal for feed preparation improved the growth performance of tilapia. Finally, we achieved secretive expression of functional BsXynE20, which can improve the nutrient utilization of a plant‐derived diet. Thus, the use of BsXynE20 may be beneficial for tilapia aquaculture.

show abstract

Bleach boosting effect of xylanase A from Bacillus halodurans C-125 in ECF bleaching of wheat straw pulp

Cited by 51 publications

References 33 publications

A comparative study of fatty acid profile and formation of biofilm inGeobacillus gargensisexposed to variable abiotic stress

A comparative study of fatty acid profile and formation of biofilm inGeobacillus gargensisexposed to variable abiotic stress

Improved efficacy of ultrafiltered xylanase–pectinase concoction in biobleaching of plywood waste soda pulp

Secretory expression and characterization of xylanase isolated from Bacillus subtilis E20 increase the utilization of plant ingredients in tilapia feed

Contact Info

Product

Resources

About