Blastulation timing is associated with differential mitochondrial content in euploid embryos

Ho, Jocelyn; Arrach, Nabil; Rhodes-Long, Katherine; Salem, Wael; McGinnis, Lynda K.; Chung, Karine; Bendikson, Kristin; Paulson, Richard J.; Ahmady, Ali

doi:10.1007/s10815-018-1113-9

Cited by 27 publications

(21 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Experiment 2, we also found that supplementation with 100, 200 and 500 ng/mL PF4 increased the number of blastocysts at day 5 compared with the number observed in our conventional culture medium with BSA. This early blastocyst development may reflect a better embryo quality and a higher subsequent embryo developmental potential [32,33].…”

Section: Discussionmentioning

confidence: 97%

The cytokine platelet factor 4 successfully replaces bovine serum albumin for the in vitro culture of porcine embryos

et al. 2020

View full text Add to dashboard Cite

The cytokine platelet factor 4 (PF4) enhances differentiation and cell viability of different stem cells lines in vitro. This study investigated whether PF4 addition to customary pig embryo semi-defined culture media can improve their developmental outcome (Experiment 1) and ultimately replace the need for bovine serum albumin (BSA, Experiment 2). Experiment 1 added PF4 (100-1000 ng/mL, 0= control) to NCSU-23 with 0.4 mg/mL BSA culturing 3,430 presumptive zygotes. Experiment 2 added PF4 (100-1000 ng/mL, 0= Control-PVA) to a BSA-free medium (NCSU-23 with 0.3 mg/mL PVA) culturing 3,820 presumptive zygotes. Zygote culture in NCSU-23 with 0.4 mg/mL BSA was used as overall control. All groups of Experiment 1 displayed similar rates of day 2-cleavage (range

show abstract

Section: Discussionmentioning

confidence: 97%

The cytokine platelet factor 4 successfully replaces bovine serum albumin for the in vitro culture of porcine embryos

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Day-6 mtDNA content was lower in blastocysts when compared with arrested embryo. And embryos with longer blastulation time had higher mtDNA content (Ho et al, 2018). Moreover, human blastocyst after vitrification exhibited significant mtDNA replication after 5 h postwarm (Perez-Sanchez et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Trophectoderm Mitochondrial DNA Content Associated with Embryo Quality and Day-5 Euploid Blastocyst Transfer Outcomes

Wang

Diao

Zhu

et al. 2021

DNA and Cell Biology

View full text Add to dashboard Cite

Mitochondria play a critical role in cell function and embryo development. Recently, increasing studies have investigated whether mitochondrial DNA (mtDNA) can be used as a predictive biomarker of embryo implantation. However, the results of its effect on implantation are still controversial. To further understand the clinical application value of mtDNA content for reproductive potential, we analyzed the influence of relative mtDNA quantity on embryo quality and transfer outcomes based on the results of second-generation sequencing of preimplantation genetic testing patients in our center. Biopsied trophectoderm (TE) from aneuploid blastocysts contained much larger amounts of mtDNA than those from euploid blastocysts ( p < 0.000). In an analysis of only euploid blastocysts (n = 769), female age had no effect on mtDNA content ( p = 0.216). TE cells biopsied on day 5 (n = 355) contained significantly higher amounts of mtDNA compared to those biopsied on day 6 (n = 388) or day 7 (n = 26) ( p < 0.000). Higher quality trophoblast was associated with lower mtDNA content ( p = 0.026), but quality of inner cell mass was not correlated with quantity of mtDNA ( p = 0.112). For transferred embryos, the biopsied date and mtDNA content were significantly associated with embryo implantation and live birth outcomes. Day-5 euploid blastocysts with lower quantities of mtDNA exhibited higher implantation rate and live birth rate. However, our data indicated that mtDNA content may not be considered an independent predictive marker, it may be a useful reference for the selection of day-5 transferred euploid blastocysts.

show abstract

“…These reports indicate that the differential mtDNA content between D-group and H-group blastocysts may be associated with different ICM and TE ratios. However, Ho et al [11] reported that the mtDNA copy number per cell obtained from biopsied TE was comparable to that obtained from whole embryos. Furthermore, another study [39] found that the mtDNA copy number did not differ between ICM and TE.…”

Section: Discussionmentioning

confidence: 97%

“…However, Ho et al . [ 11 ] reported that the mtDNA copy number per cell obtained from biopsied TE was comparable to that obtained from whole embryos. Furthermore, another study [ 39 ] found that the mtDNA copy number did not differ between ICM and TE.…”

Section: Discussionmentioning

confidence: 99%

“…Although the relationship between mitochondrial number and embryonic quality has been studied extensively in oocytes, studies in humans and mice have found that blastocysts with low mitochondrial content have high implantation capacity [5][6][7], while other studies have not detected this relationship [8][9][10]. In addition, blastocysts with chromosomal abnormalities have been reported to have greater mitochondrial numbers compared to euploid blastocysts [5,[11][12][13]; however, this relationship was not detected in other studies [7][8][9]. Therefore, more evidence is needed to confirm whether mitochondrial number can be used to evaluate embryonic quality and chromosomal abnormalities.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos

Kobayashi

Ito

Shirasuna

et al. 2020

Journal of Reproduction and Development

View full text Add to dashboard Cite

We examined the effect of ploidy on mitochondrial DNA (mtDNA) copy number in embryos and the amount of cell-free mitochondrial and nucleic DNA content (cf-mtDNA and cf-nDNA) in spent culture medium (SCM). Oocytes collected from the ovaries were matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to produce haploid or diploid embryos (H-group and D-group embryos). These embryos were cultured for 7 days, and the blastocysts and SCM were examined. The amount of mtDNA and nDNA was determined by real-time PCR. The rate of development to the blastocyst stage was higher for the D-group than for the H-group. Moreover, D-group blastocysts had less mtDNA compared to the H-group blastocysts. After activation, the mitochondrial content was constant before the blastocyst stage in D-group embryos, but increased earlier in H-group embryos. The amount of cf-mtDNA in the SCM of D-group blastocysts was greater than that of H-group blastocysts. However, when the cf-mtDNA in the SCM of 2 cell-stage embryos (day 2 post-activation) was examined, the amount of cf-mtDNA was greater in the H-group than in the D-group embryos. When D-group embryos were cultured for 7 days, a significant correlation was observed between the total cell number of blastocysts and cf-nDNA content in the SCM. Hence, although careful consideration is needed regarding the time point for evaluating mtDNA content in the embryos and SCM, this study demonstrates that mtDNA in the embryos and SCM was affected by the ploidy of the embryos.

show abstract

Blastulation timing is associated with differential mitochondrial content in euploid embryos

Cited by 27 publications

References 30 publications

The cytokine platelet factor 4 successfully replaces bovine serum albumin for the in vitro culture of porcine embryos

The cytokine platelet factor 4 successfully replaces bovine serum albumin for the in vitro culture of porcine embryos

Trophectoderm Mitochondrial DNA Content Associated with Embryo Quality and Day-5 Euploid Blastocyst Transfer Outcomes

Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos

Contact Info

Product

Resources

About