Blastoderm-differential and blastoderm-specific genes of Drosophila melanogaster

Roark, Margaret; Mahoney, Paul A.; Graham, Melissa; Lengyel, Judith A.

doi:10.1016/0012-1606(85)90473-7

Cited by 22 publications

(8 citation statements)

References 59 publications

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“…A similarly low frequency of embryospecific transcripts was found in cDNA libraries from Xenopus gastrulae (Dworkin & Dawid, 1980;Dawid etal. 1985) and Drosophila blastoderm-stage embryos (Roark, Mahoney, Graham & Lengyel, 1985; see general discussion by Davidson, 1986). The reason for this difference between the mouse and nonmammalian species is not clear but it could be related to the much slower pace of embryo development in the mammal and the need for the differentiation of structures involved in implantation.…”

Section: Developmental Patterns Of Transcript Prevalencementioning

confidence: 99%

Patterns of mRNA prevalence and expression of B1 and B2 transcripts in early mouse embryos

Taylor

Pikó

1987

Development

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Considerable evidence indicates that the 2-cell stage is a critical period of mouse embryo development when a transition from maternal to zygotic genomic control takes place. The overall changes in the structure of the mRNA population as a result of this transition were explored using a random cDNA library of 69 clones derived from late 2-cell embryos. The prevalence of the cloned sequences was analysed by dot hybridization of the cDNA clones with labelled cDNA probes synthesized to poly(A)+ RNA from different stages of development from 1-cell through blastocyst. The number of copies of individual transcripts was quantitatively estimated by comparison to standard clones of known prevalence. About one half of the transcripts that gave a measurable reaction at the 2-cell and later stages were not represented detectably in egg RNA, suggesting that a large set of zygote-specific genes not included in the maternal gene set becomes transcriptionally active in the 2-cell embryo. Six of the cDNA clones represented B1 and B2 repeat sequences. As measured by hybridization with labelled cDNA, B1 and B2 transcripts were abundantly expressed throughout cleavage, being represented by about 10(5) to 10(6) copies per embryo. However, the developmental pattern of prevalence was different for the two transcripts suggesting that their expression is regulated independently. The results of this study corroborate previous evidence derived from protein synthetic patterns and in vitro translation experiments that a major qualitative shift in the mRNA population occurs in the 2-cell embryo.

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Section: Developmental Patterns Of Transcript Prevalencementioning

confidence: 99%

Patterns of mRNA prevalence and expression of B1 and B2 transcripts in early mouse embryos

Taylor

Pikó

1987

Development

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“…Bsg25D was among the first genes to be molecularly characterized in Drosophila , and was initially reported to be transcribed only during the blastoderm stage of embryogenesis (Boyer et al, 1987; Roark et al, 1985). However, recent results with more sensitive techniques have shown that, while Bsg25D is most abundantly expressed in early embryos, it is also expressed during many developmental stages (Roy et al, 2010; Wasbrough et al, 2010; BDGP in situ homepage: ) .…”

Section: Introductionmentioning

confidence: 99%

Loss of function of theDrosophilaNinein-related centrosomal protein Bsg25D causes mitotic defects and impairs embryonic development

et al. 2016

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The centrosome-associated proteins Ninein (Nin) and Ninein-like protein (Nlp) play significant roles in microtubule stability, nucleation and anchoring at the centrosome in mammalian cells. Here, we investigate Blastoderm specific gene 25D (Bsg25D), which encodes the only Drosophila protein that is closely related to Nin and Nlp. In early embryos, we find that Bsg25D mRNA and Bsg25D protein are closely associated with centrosomes and astral microtubules. We show that sequences within the coding region and 3′UTR of Bsg25D mRNAs are important for proper localization of this transcript in oogenesis and embryogenesis. Ectopic expression of eGFP-Bsg25D from an unlocalized mRNA disrupts microtubule polarity in mid-oogenesis and compromises the distribution of the axis polarity determinant Gurken. Using total internal reflection fluorescence microscopy, we show that an N-terminal fragment of Bsg25D can bind microtubules in vitro and can move along them, predominantly toward minus-ends. While flies homozygous for a Bsg25D null mutation are viable and fertile, 70% of embryos lacking maternal and zygotic Bsg25D do not hatch and exhibit chromosome segregation defects, as well as detachment of centrosomes from mitotic spindles. We conclude that Bsg25D is a centrosomal protein that, while dispensable for viability, nevertheless helps ensure the integrity of mitotic divisions in Drosophila.

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“…It is clear from our results and those of others that a certain level of ambiguity is inherent in the initial sorting during large-scale screens. In our hands, the competition screen appeared to give reliable results with replicas of plaque-purified phage; however, the same essential technique applied to a screen of a heterogeneous genomic bank yielded approximately the same proportion of false positives [25] as in our approach which was initially differential in nature. The nature of these screens is such that well-expressed sequences are favored.…”

Section: Northern Analysis Of the Maternally-expressed D6 Sementioning

confidence: 71%

“…Similar results have been obtained by Roark et ai. [25], and Scherer et al [28], but the biological significance of the differential expression of these repetitive sequences still remains unclear. Only one sequence, copia, has been tested in an in vitro translation assay; it carries all sequence information necessary for its translation, at least in vitro [9].…”

Section: Cytological Localization Of Developmentally Regulated Sequencesmentioning

confidence: 99%

“…Several such maternally-expressed genes have been described as a result of systematic approaches using saturation mutagenesis screens [22, 301. It is also possible to isolate developmentally-regulated gene sequences by a molecular approach based solely on their differential expression. A number of stage-specific or stage-differential genes have been isolated in either differential [28] or competition [25] large-scale screens. The largest number of maternal-differential sequences reported to date have been isolated by Stephenson [31].…”

Section: Introductionmentioning

confidence: 99%

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Isolation of developmentally regulated genes in Drosophila melanogaster

Aı̈t-Ahmed

Griffin-Shea

Vola

et al. 1988

Biology of the Cell

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We used a molecular approach to search for maternally expressed genes in Drosophila melanogaster. The relative merits of differential and competition screens were analyzed in a series of reconstruction experiments using either purified phage plaques or derivative DNA sequences. In the course of this study, we isolated 5 clones whose RNA level varies during early embryogenesis. Three gastrula differential clones, b4, b8 and d3, are present in numerous copies in the genome; clone b4 hybridizes with the copia-like B104 repetitive sequence described by Scherer et al. We also isolated 2 maternally-expressed genes, not previously identified in either classical genetic or similarly molecular-based screens. These clones, b11 and d6, map at cytogenetic positions 98F and 4F respectively, on the polytene chromosome map.

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Blastoderm-differential and blastoderm-specific genes of Drosophila melanogaster

Cited by 22 publications

References 59 publications

Patterns of mRNA prevalence and expression of B1 and B2 transcripts in early mouse embryos

Patterns of mRNA prevalence and expression of B1 and B2 transcripts in early mouse embryos

Loss of function of theDrosophilaNinein-related centrosomal protein Bsg25D causes mitotic defects and impairs embryonic development

Isolation of developmentally regulated genes in Drosophila melanogaster

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