Blastocystis subtypes in patients with diabetes mellitus from the Midwest region of Brazil

Melo, Gessica Baptista de; Mazzaro, Marcia Carolina; Gomes‐Gouvêa, Michele Soares; Santos, Émelin Alves dos; Souza, Laura Vilela; Elias-Oliveira, Jefferson; Gryschek, Ronaldo Cesar Borges; Rodrigues, Raphael Silva; Paula, Fabiana Martins de

doi:10.1590/s1678-9946202163032

Cited by 7 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Human ST8 infections are rarely reported; however, a high prevalence of ST8 has been reported among primate handlers in the United Kingdom [31] and in symptomatic patients in Italy and Australia [32,33]. In Latin America, it has been identified in Brazil in patients with diabetes mellitus and in asymptomatic patients [34,35].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Molecular Characterisation of Blastocystis sp. Infecting Free-Ranging Primates in Colombia

et al. 2023

View full text Add to dashboard Cite

Infection with Blastocystis sp. has been reported in free-living and captive non-human primates (NHPs); however, surveys on Blastocystis sp. from north-western South America are scarce. This study aimed to identify Blastocystis sp. in free-ranging NHPs living in Colombia. A total of 212 faecal samples were collected from Ateles hybridus, Cebus versicolor, Alouatta seniculus, Aotus griseimembra, Sapajus apella, and Saimiri cassiquiarensis. Smears and flotation were used for morphological identification. For samples microscopically classified as positive for Blastocystis sp., we used conventional PCR to amplify and sequence two regions of the SSU rRNA gene and used Maximum Likelihood methods and Median Joining Network analyses for phylogenetic analyses. Via microscopy, 64 samples were Blastocystis sp. positive. Through molecular analyses, 18 sequences of Blastocystis sp. subtype 8 (ST8) were obtained. Strain and allele assignment together with a comparative phylogenetic approach confirmed that the sequences were ST8. Alleles 21, 156, and 157 were detected. Median Joining network analyses showed one highly frequent haplotype shared by specimens from Colombia and Peru and close relationships between haplotypes circulating in NHPs from Colombia, Ecuador, Brazil, and Mexico. This survey could support the elaboration of a more accurate epidemiological picture of the Blastocystis sp. infecting NHPs.

show abstract

Section: Discussionmentioning

confidence: 99%

Prevalence and Molecular Characterisation of Blastocystis sp. Infecting Free-Ranging Primates in Colombia

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Neste contexto, utilizamos a diagnóstico molecular como ferramenta para a detecção de DNA de Blastocystis sp. em indivíduos diabéticos e não diabéticos95 . Nesse estudo, para nossa surpresa, observamos uma positividade de 44,4% e 34,2% nos indivíduos diabéticos e não diabéticos, respectivamente.Considerando os dois grupos foram identificados os subtipos 1, 2, 3, 6, 7 e 8.Uma possibilidade de entendimento de aspectos biológicos de um organismo é realizando a sua manutenção in vivo e in vitro.…”

unclassified

Blastocystis sp.: epidemiologia molecular e relação com doenças em cenários brasileiros

Melo¹

View full text Add to dashboard Cite

Melo GB. Blastocystis sp.: molecular epidemiology and relationship with diseases in Brazilian scenarios. [thesis]. São Paulo: "Faculdade de Medicina, Universidade de São Paulo"; 2022. Blastocystis sp. is described as a protozoan prevalent in faecal samples from humans and animals, but still involves controversial aspects regarding its pathogenicity and epidemiology. In Brazil, there are few studies related to the understanding of relevant aspects of this enigmatic organism. Therefore, different approaches were proposed, such as the overview of research in Brazil in the last 20 years; the occurrence of Blastocystis sp. in the HC complex (Hospital das Clínicas) in the period of 2016 to 2021; the molecular characterization of isolates from patients with urticaria and diabetes, and perform in vitro cultivation of human isolates. The data of the literature review on the research of Blastocystis sp. in Brazil regarding the evaluation of its positivity in the HC-FMUSP complex reinforce the presence of this organism in our environment and raises the possibility of further studies to understand its real clinical significance. In relation to urticaria, it was not possible to establish a direct relationship with Blastocystis sp.Infection. Yet, it was possible to identify high intra and inter-subtypes diversity circulating in these patients. Regarding diabetics, although this protist was more prevalent in this group, it has not yet been possible to establish a relationship between diabetes and Blastocystis sp. Finally, our findings on the in vitro maintenance of human isolates allowed us to observed different cultivation times and forms present in different subtypes and alleles. Thus, further studies are needed focusing on the interaction between Blastocystis sp. and the host, to understand the impact of this organism on public health.

show abstract

An update on the distribution of Blastocystis subtypes in the Americas

Jiménez¹,

Muñoz²,

Ramírez³

2022

Heliyon

View full text Add to dashboard Cite

Blastocystis subtypes in patients with diabetes mellitus from the Midwest region of Brazil

Cited by 7 publications

References 31 publications

Prevalence and Molecular Characterisation of Blastocystis sp. Infecting Free-Ranging Primates in Colombia

Prevalence and Molecular Characterisation of Blastocystis sp. Infecting Free-Ranging Primates in Colombia

Blastocystis sp.: epidemiologia molecular e relação com doenças em cenários brasileiros

An update on the distribution of Blastocystis subtypes in the Americas

Contact Info

Product

Resources

About