Blastocystis in ulcerative colitis patients: Genetic diversity and analysis of laboratory findings

Çoşkun, Adil; Malatyalı, Erdoğan; Ertabaklar, Hatice; Yaşar, Mustafa; Karaoğlu, Ali Önder; Ertuğ, Sema

doi:10.1016/j.apjtm.2016.07.018

Cited by 20 publications

(10 citation statements)

References 24 publications

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“…In addition, Blastocystis colonization was significantly associated with high bacterial richness and Bacteroides enterotype and it was suggested that Blastocystis might be an indicator of a healthy intestinal flora rather than a dysbiosis (34,35). On the other hand, decreased Blastocystis colonization was reported in gastrointestinal disorders including inflammatory bowel diseases, ulcerative colitis, and irritable bowel syndrome (36)(37)(38).…”

Section: Discussionmentioning

confidence: 99%

First Molecular Characterisation of <i>Blastocystis</i> from Experimental Rats in Turkey and Comparison of the Frequencies Between Obese and Non-obese Groups

Malatyalı¹,

Başaran²,

Gökçimen³

et al. 2021

Turkiye Parazitol Derg

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Blastocystis is a zoonotic protozoan that infects a wide range of animals, including humans and rodents. This study aimed to determine the frequency and subtype distribution of Blastocystis in laboratory rats at a laboratory animal facility in Turkey. Methods:This study included 54 male Sprague-Dawley rats from Aydın Adnan Menderes University Laboratory Animal Center. Among these rats, 30 were fed with high-fat diet (obese group) and the remaining 24 received standard chow (non-obese group). Blastocystis positivity was determined with amplification of small subunit 18S rRNA gene following their nucleic acid extraction from faecal samples. Subtypes were detected by submitting the partial 18S rRNA gene sequences to the database (pubmlst. org/blastocystis). The phylogenetic tree from evolutionary distance data was constructed using the neighbour-joining method.Results: Blastocystis infection was detected in 33 (61.1%) of 54 laboratory rats. The frequency of Blastocystis was significantly different between obese and non-obese rats (p<0.05), with 43.3% and 83.3%, respectively. When referred to the database, exact matches were identified with Blastocystis subtype 4 (ST4) for all isolates. In the phylogenetic analysis of the partial 18S rDNA sequence, the sequence was closely clustered with reference ST4 subtypes from other countries, including China, Japan, United Kingdom and Czech Republic. Conclusion:This study revealed the high rate of Blastocystis colonisation in laboratory rats, posing a risk for human transmission.The comparison of obese and non-obese groups supported the idea that Blastocystis might be an indicator of healthy gut flora. The detection of ST4 in all rats agreed with previous reports of the predominance of this subtype in rodents.

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Section: Discussionmentioning

confidence: 99%

First Molecular Characterisation of <i>Blastocystis</i> from Experimental Rats in Turkey and Comparison of the Frequencies Between Obese and Non-obese Groups

Malatyalı¹,

Başaran²,

Gökçimen³

et al. 2021

Turkiye Parazitol Derg

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“…For instance, the high sensitivity of real-time PCR tests has helped us understand that some parasites are-on an overall basis-much more common than previously anticipated, with prevalence rates approaching 100% in some communities, even in developed countries. Examples include Blastocystis and Dientamoeba, which appear to be more or less obligate eukaryotic members of the gut microbiota in some populations [15][16][17][18][19][20][21], while less frequent in others, including individuals with functional and organic bowel diseases and metabolic disorders [13,[22][23][24][25]. For instance, we recently showed Dientamoeba to be a consistent finding in the stool from children in childcare in Denmark [20], and similarly that Blastocystis is a frequent finding in children in Nigeria, with prevalence increasing by age [18].…”

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confidence: 96%

Associations between Gut Microbiota and Common Luminal Intestinal Parasites

Stensvold

Giezen

2018

Trends in Parasitology

144

136

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The development and integration of DNA-based methods in research and clinical microbiology laboratories have enabled standardised and comprehensive detection and differentiation of the microbes colonising our guts. For instance, the single-celled parasites Blastocystis and Dientamoeba appear to be much more common than previously thought, especially so in healthy individuals. While increasing evidence appears to suggest limited pathogenicity of these parasites, next-generation-sequencing-based studies have helped us to appreciate links between parasite colonisation and certain host phenotypical characteristics and gut microbial profiles. The fundamental question remains as to whether such parasites are merely indicators or active manipulators of gut microbiota structure and function. In this article, we collate existing evidence that these parasites are, at minimum, indicators of intestinal microbiota structure.

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“…As B. hominis prevalence has been found to be greater in the healthy groups than in patients with UC in most studies, this suggests that B. hominis has a protective role rather than being a cause of UC and could be a marker of a healthy microbiota (23,30,32,33). In a healthy microbiota, the butyrate produced by bacteria is used for ATP production with β-oxidation by colon epithelial cells.…”

Section: Discussionmentioning

confidence: 96%

“…In contrast to these studies, Coşkun et al (30) examined B. hominis prevalence in the fecal cultures of 150 patients with UC and found it to be lower in active patients than in those in remission (active UC=3.8% vs. remission=11.8%, p<0.05).…”

Section: Discussionmentioning

confidence: 98%

The role of Blastocystis hominis in the activation of ulcerative colitis

Kök

Çekın

et al. 2018

Turk J Gastroenterol

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Background/Aims: Several studies have shown that a change in microbiota plays an important role in the pathogenesis of inflammatory bowel disease (IBD). Furthermore, with the emergence in recent studies of differences according to the subtype of IBD and whether the disease is active or in remission, there has started to be research into the relationship between IBD and several microorganisms. Blastocystis hominis is primary among these organisms. The aim of the present study was to determine the role of B. hominis in the acute flare-up of ulcerative colitis (UC). Materials and Methods: A total of 114 patients with UC were included in the study, with 52 in the active phase. The Mayo scoring system was used for the activity index. Patients determined with a flare-up agent other than B. hominis were excluded from the study. Fecal samples of the patients were examined by the polymerase chain reaction method for the presence of B. hominis. Results: B. hominis positivity was determined in 37 (34%) patients with UC. Of the patients, 17 (32.6%) were in the acute flare-up phase, and 20 (32.2%) were in remission (p=0.961). In 11 (64.7%) of the B. hominis positive patients, the disease severity was determined as mild-moderate (p<0.001). Conclusion: The results of the present study showed that while there was no difference between the active and remission phases in respect of B. hominis presence, there was milder involvement in those determined with B. hominis.

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Blastocystis in ulcerative colitis patients: Genetic diversity and analysis of laboratory findings

Abstract: The present study confirms previous findings that have indicated the predominance of Blastocystis ST3 in humans and contributes additional evidence that suggests the low colonisation of Blastocystis infection in ulcerative colitis patients during active stage.

Cited by 20 publications

References 24 publications

First Molecular Characterisation of <i>Blastocystis</i> from Experimental Rats in Turkey and Comparison of the Frequencies Between Obese and Non-obese Groups

First Molecular Characterisation of <i>Blastocystis</i> from Experimental Rats in Turkey and Comparison of the Frequencies Between Obese and Non-obese Groups

Associations between Gut Microbiota and Common Luminal Intestinal Parasites

The role of Blastocystis hominis in the activation of ulcerative colitis

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