Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching

Barnes, Frank L.; Crombie, Amanda; Gardner, David K.; Kausche, Annette; Lacham-Kaplan, Orly; Suikkari, Anne‐Maria; Tiglias, Jennifer; Wood, Carl; Trounson, Alan

doi:10.1093/oxfordjournals.humrep.a135896

Cited by 269 publications

(82 citation statements)

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“…The administration of testosterone propionate had a virilizing effect on the external genitalia of female lambs as described previously (Barnes et al 1995, Unsworth et al 2005. Testosterone-treated lambs had genitalia that were very similar to males, with a penis and scrotal tissue, but with no testicular tissue.…”

Section: Effect Of Prenatal Androgen Exposure On External Appearance mentioning

confidence: 54%

Disordered follicle development in ovaries of prenatally androgenized ewes

Forsdike¹,

Hardy²,

Bull³

et al. 2007

Journal of Endocrinology

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Exposure to excess androgens in utero induces irreversible changes in gonadotrophin secretion and results in disrupted reproductive endocrine and ovarian function in adulthood, in a manner reminiscent of the common clinical endocrinopathy of polycystic ovary syndrome (PCOS). We have recently identified an abnormality in early follicle development in PCOS which we suggested might be an androgenic effect. We propose that altered ovarian function in androgenized ewes is due to prenatal androgens not only causing an abnormality of gonadotrophin secretion, but also exerting a direct effect on the early stages of folliculogenesis. Therefore, in this study, we explored the possible differences between small preantral follicles in the ovarian cortex of androgenized female lambs with those of normal lambs. At 8 months of age, small ovarian cortical biopsies (approximately 5 mm 3

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Section: Effect Of Prenatal Androgen Exposure On External Appearance mentioning

confidence: 54%

Disordered follicle development in ovaries of prenatally androgenized ewes

Forsdike¹,

Hardy²,

Bull³

et al. 2007

Journal of Endocrinology

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“…A plausible reason for the hampered development of NT embryos may be that the remodelling of foreign nuclei into an active zygotic genome is not totally successful. When suitable media are used, parthenogenetic embryos maintain their own gametogenetic content and can develop until the blastocyst stage (Barnes et al, 1995;Bavister, 1995). Kishikawa et al (1999) reported rates of morula/blastocyst formation of 58% for mouse NT embryos versus 98% for parthenogenetic control embryos.…”

Section: Discussionmentioning

confidence: 99%

“…The KSOM medium (Erbach et al, 1994) was prepared in the laboratory; it had a low glucose content (0.2 mM) and was supplemented with 4 mg/mL BSA. The G1 and G2 media (Barnes et al, 1995) were commercially obtained (Scandinavian IVF, Gotheburg, Sweden). KSOM, as well as G1 and G2, had an osmolarity of 265 mOsm.…”

Section: Mediamentioning

confidence: 99%

Effect of Culture Media on In Vitro Development of Cloned Mouse Embryos

Heindryckx

Rybouchkin²,

Elst³

et al. 2001

Cloning

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The effect of simple and sequential embryo culture media on the preimplantation development of mouse nuclear transfer (NT) embryos reconstructed with cumulus cell nuclei using a mechanical NT technique was studied. Blastocyst formation rate was evaluated using CZB medium and the sequential media G1/G2 and KSOM/G2. Arrested two-and three-cell NT embryos were Hoechst-stained to check for nuclear abnormalities. Nonmanipulated and sham-manipulated parthenogenetic embryos served as controls for, respectively, the medium and the handling technique. Rates of blastocyst formation for medium and handling control embryos were similar in CZB (58% and 61%), in G1/G2 (94% and 85%), and in KSOM/G2 (88% and 84%). Development of NT embryos was significantly impaired from the two-cell stage onwards, reaching the blastocyst stage at a rate of 5% in CZB, 14% in G1/G2, and 28% in KSOM/G2. Arrested two-and three-cell stage NT embryos showed a high rate of binucleation. These data demonstrate not only that NT embryos are more sensitive to in vitro culture conditions than parthenogenetic control embryos but also that selection of culture media can influence the preimplantation development of NT embryos. 41

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“…Only a few reports have been published for IVM of human oocytes from nonstimulated cycles in women, leading to term development (9,17,60,78,90). Oocyte viability and embryonic development after IVM are substantially lower than with VVM.…”

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confidence: 99%

Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome

Lee¹,

VandeVoort

Gaughan

et al. 2011

American Journal of Physiology-Endocrinology and Metabolism

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Lee YS, VandeVoort CA, Gaughan JP, Midic U, Obradovic Z, Latham KE. Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome. Am J Physiol Endocrinol Metab 301: E196 -E209, 2011. First published April 12, 2011 doi:10.1152/ajpendo.00686.2010.-The elaboration of a quality oocyte is integrally linked to the correct developmental progression of cumulus cell phenotype. In humans and nonhuman primates, oocyte quality is diminished with in vitro maturation. To determine the changes in gene expression in rhesus monkey cumulus cells (CC) that occur during the final day prior to oocyte maturation and how these changes differ between in vitro (IVM) and in vivo maturation (VVM), we completed a detailed comparison of transcriptomes using the Affymetrix gene array. We observed a large number of genes differing in expression when comparing IVM-CC and VVM-CC directly but a much larger number of differences when comparing the transitions from the prematuration to the post-IVM and post-VVM states. We observed a truncation or delay in the normal pattern of gene regulation but also remarkable compensatory changes in gene expression during IVM. Among the genes affected by IVM are those that contribute to productive cell-cell interactions between cumulus cell and oocyte and between cumulus cells. Numerous genes involved in lipid metabolism are incorrectly regulated during IVM, and the synthesis of sex hormones appears not to be suppressed during IVM. We identified a panel of 24 marker genes, the expression of which should provide the foundation for understanding how IVM can be improved for monitoring IVM conditions and for diagnosing oocyte quality. oocyte quality; in vitro maturation LIFE BEGINS IN THE OOCYTE. The oocyte accumulates a remarkable and complex macromolecular reservoir that mediates and controls the initial development of each new individual while at the same time eliminating a range of molecules that promote gametogenesis but would otherwise impede early embryogenesis (8,36,37,67). Failure to complete these transitions appropriately yields an oocyte with limited developmental potential, unable to undergo fertilization or activation, or unable to sustain embryogenesis after fertilization and activation.Because of the key role of the oocyte in early development, understanding the molecular features that confer a high oocyte developmental potential is of paramount importance in reproductive biology. Such information will reveal the underlying mechanisms and processes that drive oogenesis and early embryogenesis. Moreover, such information will provide markers with which to screen and select high-quality oocytes for assisted reproduction in humans and could enhance success in agricultural reproductive biology and species conservation applications.The oocyte develops in close coordination with the somatic companion cells, particularly the cumulus oophorous cells, which maintain direct contact with the oocyte via transzonal processes and gap junctions until ovulation. Through its secretion...

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Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching

Cited by 269 publications

References 0 publications

Disordered follicle development in ovaries of prenatally androgenized ewes

Disordered follicle development in ovaries of prenatally androgenized ewes

Effect of Culture Media on In Vitro Development of Cloned Mouse Embryos

Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome

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