Blastocyst culture and cryopreservation to optimize clinical outcomes of warming cycles

Zhu, Lixia; Xi, Qingsong; Zhang, Hanwang; Li, Yufeng; Ai, Jihui; Jin, Liu

doi:10.1016/j.rbmo.2013.04.006

Cited by 41 publications

(26 citation statements)

References 31 publications

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“…However, cumulative clinical pregnancy rates from cleavage stages (derived from fresh and thaw cycles) were higher than rates from blastocyst cycles [Glujovsky et al 2012]. Blastocyst culture as an embryo selection tool does not improve embryo viability but it could help patients to achieve pregnancy more quickly [Zhu et al 2013]. Our observations showed that blastocyst transfer increased pregnancy and implantation rates compared with transfer of cleavage-stage embryos only in patients of 535 years of age, which is consistent with the opinion of the ASRM Practice Committee [ASRM 2013].…”

Section: Discussionmentioning

confidence: 99%

The effects of fertilization mode, embryo morphology at day 3, and female age on blastocyst formation and the clinical outcomes

Yin

Jiang

et al. 2014

Systems Biology in Reproductive Medicine

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(2015) The effects of fertilization mode, embryo morphology at day 3, and female age on blastocyst formation and the clinical outcomes, Systems Biology in Reproductive Medicine, 61:1, 50-56, DOI: 10.3109/19396368.2014 AbstractThe aim of this study was to evaluate the influence of in vitro fertilization (IVF) versus intracytoplasmic sperm injection (ICSI), fertilization mode embryonic morphology at day 3, and female age on blastocyst development, on the clinical outcomes of pregnancy after blastocyst transfer. A total of 471 cycles were retrospectively investigated. The rates of blastocyst formation and of good blastocyst morphology were higher in IVF than in ICSI cycles but there were no significant differences in the clinical pregnancies or in the miscarriage rates. The rates of formation of blastocyst and of blastocysts with good morphology were significantly higher from good-morphology embryos than from poor-morphology embryos. Nevertheless, 16.9% of the poor-morphology embryos reached the blastocyst stage. The total rates of blastocyst formation, and rates of clinical pregnancy and implantation were statistically similar in the age 535, 35-39, and 439 year groups, although tending to decrease with increasing age. When equal numbers of embryos were transferred on day 3, the rates of clinical pregnancy and implantation after blastocyst transfer were significantly higher in the 535 year age group than in the 35-39 and 439 year age groups, which were not significantly different. The miscarriage rates after embryo or blastocyst transfers were not statistically different in groups of similar age. Therefore, extended embryo culture up to the blastocyst stage could be implemented for women aged younger than 35 years to increase the pregnancy rate. For older women, transfer and vitrification of available embryos at day 3 and extended culture of morphologically poor embryos to the blastocyst stage for cryopreservation may improve the clinical outcome.

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Section: Discussionmentioning

confidence: 99%

The effects of fertilization mode, embryo morphology at day 3, and female age on blastocyst formation and the clinical outcomes

Yin

Jiang

et al. 2014

Systems Biology in Reproductive Medicine

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“…There has been considerable debate about clinical outcomes of frozen-thawed embryo transfer and frozen-thawed blastocyst transfer [Moragianni et al 2010;Han et al 2012]. However, many studies have indicated that frozen-thawed blastocyst transfer has better pregnancy outcomes [Roy et al 2014;Tong et al 2012;Zhu et al 2013]. This suggests that blastocysts are cleavage-stage embryos with development potential, and blastocyst culture can effectively screen for these embryos.…”

Section: Discussionmentioning

confidence: 99%

Clinical outcomes of frozen-thawed single blastocyst transfer in patients requiring whole embryo freezing

Wang

Liu

et al. 2016

Systems Biology in Reproductive Medicine

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We compared clinical outcomes amongst frozen-thawed cleavage-stage embryo, double and single blastocyst transfers in patients requiring whole embryo freezing. Data of infertile patients undergoing in-vitro fertilization and embryo transfer (IVF-ET) in our Reproductive Medicine Center from January 2010 to December 2012 were retrospectively analyzed. According to patients' wishes, patients were divided into cleavage-stage embryo transfer groups (group A, n = 456), double blastocyst transfer group (group B, n = 106), and single blastocyst transfer group (group C, n = 402). We found that the number of frozen embryos was significantly less in groups B and C than in group A (all p < 0.05), but the implantation rate was significantly higher in groups B and C as compared to group A (all p < 0.05). The clinical pregnancy rate and pregnancy rate per included cycle were significantly higher in group B than in groups A and C (all p < 0.05). The multiple pregnancy rate was significantly lower in group C than in groups A and B (all p < 0.05). The rate of early abortion was significantly lower in group C as compared to group A (p < 0.05). The data support the view that it may be the best clinical strategy for patients who require whole embryo freezing and have four or more Day 3 embryos available, to incubate Day 3 embryos into blastocysts, which are then vitrified for elective single blastocyst transfer.

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“…The methods of ovarian stimulation protocols, sperm preparation, IVF embryo culture, and FET protocols in our reproduction medicine center have been described elsewhere. [ 13 – 15 ] Briefly, ovarian stimulation protocols were chosen according to the maternal age, cause of infertility, ovarian response, and coexisting medical conditions. Recombinant human chorionic gonadotropin (hCG) (250 mg; Ovidrel; Serono; Geneva) was administered to trigger ovulation when 2 leading follicles reached a mean diameter of 18 mm.…”

Section: Methodsmentioning

confidence: 99%

Endometrial thickness as a predictor of the reproductive outcomes in fresh and frozen embryo transfer cycles

Zhang

Li²,

Ren³

et al. 2018

Medicine

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To evaluate the relationship between endometrial thickness during fresh in vitro fertilization (IVF) cycles and the clinical outcomes of subsequent frozen embryo transfer (FET) cycles.FET cycles using at least one morphological good-quality blastocyst conducted between 2012 and 2013 at a university-based reproductive center were reviewed retrospectively. Endometrial ultrasonographic characteristics were recorded both on the oocyte retrieval day and on the day of progesterone supplementation in FET cycles. Clinical pregnancy rate, spontaneous abortion rate, and live birth rate were analyzed.One thousand five hundred twelve FET cycles was included. The results showed that significant difference in endometrial thickness on day of oocyte retrieval (P = .03) was observed between the live birth group (n = 844) and no live birth group (n = 668), while no significant difference in FET endometrial thickness was found (P = .261) between the live birth group and no live birth group. For endometrial thickness on oocyte retrieval day, clinical pregnancy rate ranged from 50.0% among patients with an endometrial thickness of ≤6 mm to 84.2% among patients with an endometrial thickness of >16 mm, with live birth rate from 33.3% to 63.2%. Multiple logistic regression analysis of factors related to live birth indicated endometrial thickness on oocyte retrieval day was associated with improved live birth rate (OR was 1.069, 95% CI: 1.011–1.130, P = .019), while FET endometrial thickness did not contribute significantly to pregnancy outcomes following FET cycles. The ROC curves revealed the cut-off points of endometrial thickness on oocyte retrieval day was 8.75 mm for live birth.Endometrial thickness during fresh IVF cycles was a better predictor of endometrial receptivity in subsequent FET cycles than FET cycle endometrial thickness. For those females with thin endometrium in fresh cycles, additional estradiol stimulation might be helpful for adequate endometrial development.

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Blastocyst culture and cryopreservation to optimize clinical outcomes of warming cycles

Cited by 41 publications

References 31 publications

The effects of fertilization mode, embryo morphology at day 3, and female age on blastocyst formation and the clinical outcomes

The effects of fertilization mode, embryo morphology at day 3, and female age on blastocyst formation and the clinical outcomes

Clinical outcomes of frozen-thawed single blastocyst transfer in patients requiring whole embryo freezing

Endometrial thickness as a predictor of the reproductive outcomes in fresh and frozen embryo transfer cycles

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