Blastobotrys, Sporothrix and Trichosporiella: generic delimitation, new species, and a Stephanoascus teleomorph

Abstract

The morphological and physiological characters of the species of Blastobotrys, and of a number of similar species of Sporothrix, Trichosporiella and Candida were studied. Blastobotrys is defined as having mother cells (primary conidia) which form distinct, secondary conidia. Sporothrix has non-catenulate conidia, or the conidia are catenulate without marked differentiation of conidia of first and second order. Trichosporiella is delimited from Candida by a stronger coherence between hyphal cells, arthroconidia being absent. Four new Blastobotrys species are described, and two unnamed species; two new combinations are proposed in Trichosporiella. Related species in other genera are briefly discussed. A new Stephanoascus teleomorph is described in two strains originally identified as Sp. fungorum. Two diagnostic keys to the described taxa, using either morphological or physiological characters, are given.

Peritonitis Due to Blastobotrys proliferans in a Patient Undergoing Continuous Ambulatory Peritoneal Dialysis

“…1c) are present. Blastobotrys proliferans is different from all other Blastobotrys species by its proliferating mother cells and the refractive bodies in those mother cells (8). It grows with most carbon sources, does not assimilate nitrate, and ferments glucose.…”

“…Identification of the species Blastobotrys proliferans Marvanová was done based on the carbon assimilation pattern (ID32C and 50CH; bioMérieux) and microscopic morphology after slide culture in 2% malt agar medium after 6 days at 25°C using the keys established by de Hoog and colleagues (8,9). Sequences were determined for the internal transcribed spacer 1 (ITS1)-5.8S-ITS2 regions (GenBank accession no.…”

The divorce of Sporothrix and Ophiostoma: solution to a problematic relationship

“…Based on his work, many later authors treated asexual morphs previously ascribed to all the genera referred to above, in Sporothrix (Samuels and Müller, 1978, Domsch et al., 1980, Upadhyay, 1981, de Hoog, 1993). Several additional asexual species were also described in Sporothrix from a variety of hosts (de Hoog, 1978, de Hoog and Constantinescu, 1981 Moustafa, 1981, de Hoog et al., 1985, Constantinescu & Ryman 1989, and more). By the middle 1980's, evidence that Sporothrix is not a homogenous group, and that some of the species have basidiomycete affiliations, began to appear (Smith and Batenburg-Van der Vegte, 1985, Weijman and de Hoog, 1985, de Hoog, 1993).…”

Morphological taxonomy of little-differentiated Hyphomycetes

“…When present, the yeast-like state of several Blastobotrys species is rather undiagnostic, but the hyphal state of the same organism produces conidiophores and conidium mother-cells of characteristic structure. The latter characters are supported by minute physiological differences (De Hoog et al, 1985).…”