Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers

Yadav, Manoj Kumar; Aravindan, S; Ngangkham, Umakanta; Raghu, S.; Prabhukarthikeyan, S.R.; Keerthana, U.; Marndi, Bishnu Charan; Adak, Totan; Munda, Sushmita; Deshmukh, Rupesh; Pramesh, D.; Samantaray, Sanghamitra; Rath, Prakash Chandra

doi:10.1371/journal.pone.0211061

Cited by 47 publications

(33 citation statements)

References 62 publications

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“…As expected, the studied markers showed, in general, low allelic diversity. Our results corroborate with the previous findings of Yadav et al (2019), who reported that the average number of alleles was two and the mean gene diversity was 32%. In the current study, among the 10 genotypes screened for the presence of major blast resistance genes, at least one genotype had one allele of the eight major R genes (Table 6).…”

Section: Genotype Identification and Allelic Diversitysupporting

confidence: 93%

“…Additionally, four markers were identified in Sakha104, Sakha108, and Eg-N-3. The use of molecular markers for the identification of rice genotypes carrying resistance genes is well-established in different geographic regions (Liu et al, 2015;Zhang et al, 2015;Yadav et al, 2019); however, in Egypt and Saudi Arabia, research related to the identification of resistance genes in rice is still in preliminary stages.…”

Section: Genotype Identification and Allelic Diversitymentioning

confidence: 99%

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Molecular identification of blast resistance genes in rice genotypes using gene-specific markers

Aldaej¹,

Ismail²,

Rezk³

et al. 2019

bta

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Molecular identification of major blast resistance (R ) genes in rice was performed in a group of 10 rice (Oryza sativa) genotypes from Egypt and Saudi Arabia using six DNA markers (T8042, NSB, YL153/YL154, RM3843, RM3330, and z4794) belonging to three classes: single-nucleotide polymorphisms (SNPs), simple sequence repeats (SSRs), and insertion-deletions (InDels). The markers were chosen based on their linkage to six major R genes (Pit, Pib, Pi39 (t), Pi40 (t), Piz/Piz-t, and Pita /Pita2 ). The studied markers showed low allelic diversity, with the number of alleles identified in a single genotype ranging between two and nine alleles. The Egyptian genotype Eg-N-7 had two alleles of the Pi40 (t) gene, while the Saudi Arabian genotype Al-Ahsa1 had nine alleles that belonged to the seven R genes. The studied genotypes were also assessed for 19 agro-morphological traits.Analysis of variance of the studied traits showed significant differences among the genotypes. The putative associations between molecular markers and the agro-morphological traits were examined using associationmapping approach by employing the unified mixed model. Eight significant marker-trait associations were detected for eight agro-morphological traits (plant height, panicle length, sterility percentage, grain width, grain shape, elongation percentage, gelatinization temperature, and days to maturity). The phenotypic variance shown by each marker ranged between 43% and 65%. The findings of the current study will assist in identifying possible blast resistance genotypes for future rice breeding programs. Additionally, the results highlight the possible dual usage of specific markers in genotypic screening as well as in determining marker-trait associations.

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Section: Genotype Identification and Allelic Diversitysupporting

confidence: 93%

Section: Genotype Identification and Allelic Diversitymentioning

confidence: 99%

Molecular identification of blast resistance genes in rice genotypes using gene-specific markers

Aldaej¹,

Ismail²,

Rezk³

et al. 2019

bta

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“…In a more recent study, Yan et al (2017) reported the genetic frequencies of 11 R genes, ranging from 9.4 to 100.0% in 32 Chinese rice germplasms while Yadav et al (2019) reported the genetic frequencies ranged between 4.96 and 100.0% in 161 Indian rice landraces. Prior to this study, Hannum et al (2018) have screened 15 rice accessions originating from other districts in North Sumatra and reported higher genetic frequencies from 47.0 to 100.0%.…”

Section: Discussionmentioning

confidence: 99%

Screening of Blast Resistance Genes from South Tapanuli Rice Cultivars, North Sumatra, Indonesia

Hannum

Wahyuningsih

Sinaga

et al. 2020

OnLine Journal of Biological Sciences

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Rice blast disease caused by a phytopathogenic fungus, Magnaporthe oryzae, has been considered as a major threat to global rice productivity, including Indonesia. South Tapanuli is a regency in North Sumatra, known for its intensive and significant rice production activities. A collection of 13 rice cultivars originating from the region, is claimed for its resistances against the rice blast disease based on the practices by the local farmers. Molecular screening of blast resistance (R) genes was initiated to the 13 accessions using the Polymerase Chain Reaction (PCR) targeting five blast resistance (R) genes, Pup1, Pi-37, Pi-d2, Pi-ta 2 , Pib and Pi-kh. Out of the 13 accessions, the Pi-d2, Pi-ta 2 and Pib were detected in all cultivars, showing 100% of R gene frequency. The second dominant, Pup1 and Pi-37 gene frequencies were 76.9 and 61.5%, respectively while the least dominant, Pi-kh gene frequency was 15.4%. The number of R genes detected among cultivars was ranged between 3 to 6 genes. The genetic associations among thirteen germplasms were determined using unweighted pair group method with arithmetic mean (UPGMA) analysis. Cluster analysis revealed that the least blast-resistant cultivars: Putri Kembar, Silatian and Siganteng, were grouped into cluster 1, showing polymorphism for Pi-d2, Pi-ta 2 and Pi-b. The high-resistant cultivars, Martabe-Sicondong and Sayuti, Sitolas, Pulo Raja, Pulo Pandan, Siporang and Pulo Manggis, were grouped into cluster 2 and 3, respectively. The medium blast-resistant cultivars, IR 64 and Sitampan were grouped into cluster 4, showing polymorphism for Pup1, Pi-d2, Pi-ta 2 and Pi-b. These results indicated that the utilization of blast-resistant cultivars in North Tapanuli was supported by the presence of R genes.

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“…In another research article, molecular analysis of variance of landraces originated from nine diverse rice ecologies of India showed maximum (93%) diversity within the population and least (7%) between populations. Five markers like K3957, Pikh, Pi2-i, RM212and RM302 were strongly associated with blast disease with the phenotypic variance of 1.4% to 7.6% [190].…”

Section: Marker-based Breeding Methodsmentioning

confidence: 99%

Rice Blast Disease in India: Present Status and Future Challenges

Annegowda¹,

Prasannakumar²,

Mahesh³

et al. 2022

Integrative Advances in Rice Research

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Rice (Oryza sativa L.) is the staple food of the majority of Indians, and India is both the major producer and consumer of rice. Rice cultivation in India is confronted with diverse agro-climatic conditions, varying soil types, and several biotic and abiotic constraints. Among major fungal diseases of Rice in India, the blast caused by Magnaporthe oryzae is the most devastating disease, with the neck blast being the most destructive form. Most of the blast epidemic areas in India have been identified with a mixture of races blast fungus resulting in the resistance breakdown in a short period. At present, a more significant number of the rice varieties cultivated in India were bred by conventional breeding methods with blast resistance conferred by a single resistance gene. Therefore, the blast disease in India is predominantly addressed by the use of ecologically toxic fungicides. In line with the rest of the world, the Indian scientific community has proven its role by identifying several blast resistance genes and successfully pyramiding multiple blast resistance genes. Despite the wealth of information on resistance genes and the availability of biotechnology tools, not a great number of rice varieties in India harbor multiple resistance genes. In the recent past, a shift in the management of blast disease in India has been witnessed with a greater focus on basic research and modern breeding tools such as marker-assisted selection, marker-assisted backcross breeding, and gene pyramiding.

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Blast resistance in Indian rice landraces: Genetic dissection by gene specific markers

Cited by 47 publications

References 62 publications

Molecular identification of blast resistance genes in rice genotypes using gene-specific markers

Molecular identification of blast resistance genes in rice genotypes using gene-specific markers

Screening of Blast Resistance Genes from South Tapanuli Rice Cultivars, North Sumatra, Indonesia

Rice Blast Disease in India: Present Status and Future Challenges

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