Bladder muscular wall regeneration with autologous adipose mesenchymal stem cells on three-dimensional collagen-based tissue-engineered prepuce and biocompatible nanofibrillar scaffold

Kajbafzadeh, Abdol‐Mohammad; Tourchi, Ali; Mousavian, Amir-Abbas; Rouhi, Leila; Tavangar, Seyed Mohammad; Sabetkish, Nastaran

doi:10.1016/j.jpurol.2014.03.010

Cited by 30 publications

(21 citation statements)

References 22 publications

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“…However, we have successfully acellularized and recellularized the whole rat lung by the application of a simple method in our recent study (Kajbafzadeh et al . ). In the present study, the lung was perfectly decellularized by pulmonary and bronchial arteries perfusion without dissection of the whole organ.…”

Section: Discussionmentioning

confidence: 97%

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

Kajbafzadeh

Khorramirouz

Akbarzadeh

et al. 2015

Int J Experimental Path

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The aim of this study was to develop a method to generate multi-organ acellular matrices. Using a foetal sheep model have developed a method of systemic pulsatile perfusion via the umbilical artery which allows for simultaneous multi-organ decellularization. Twenty sheep foetuses were systemically perfused with Triton X-100 and sodium dodecyl sulphate. Following completion of the whole-body decellularization, multiple biopsy samples were taken from different parts of 21 organs to ascertain complete cell component removal in the preserved extracellular matrices. Both the natural and decellularized organs were subjected to several examinations. The samples were obtained from the skin, eye, ear, nose, throat, cardiovascular, respiratory, gastrointestinal, urinary, musculoskeletal, central nervous and peripheral nervous systems. The histological results depicted well-preserved extracellular matrix (ECM) integrity and intact vascular structures, without any evidence of residual cellular materials, in all decellularized bioscaffolds. Scanning electron microscope (SEM) and biochemical properties remained intact, similar to their age-matched native counterparts. Preservation of the collagen structure was evaluated by a hydroxyproline assay. Dense organs such as bone and muscle were also completely decellularized, with a preserved ECM structure. Thus, as shown in this study, several organs and different tissues were decellularized using a perfusion-based method, which has not been previously accomplished. Given the technical challenges that exist for the efficient generation of biological scaffolds, the current results may pave the way for obtaining a variety of decellularized scaffolds from a single donor. In this study, there have been unique responses to the single acellularization protocol in foetuses, which may reflect the homogeneity of tissues and organs in the developing foetal body.

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Section: Discussionmentioning

confidence: 97%

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

Kajbafzadeh

Khorramirouz

Akbarzadeh

et al. 2015

Int J Experimental Path

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“…Unmodified ASCs can be seeded on different scaffolds to replace the urinary tract. In vivo differentiation of ASCs into SMCs has been observed after delivery of unmodified human ASCs within the bladder wall of nude mice [64], or autologous rat ASCs seeded on scaffold [65]. However, this phenotypic conversion occurs for a small percentage of the delivered ASCs.…”

Section: Undifferentiated Mesenchymal Stem Cellsmentioning

confidence: 99%

“…ASCs have been used for in vivo studies for urinary bladder replacement or augmentation whether unmodified [96,65] or after differentiating them into SMC [73]. Unmodified autologous ASCs were seeded on acellular matrices (bladder acellular matrix in rabbits or prepucial matrix in rats) and showed increase in smooth muscle content in the seeded group and improved bladder functional evaluation when compared to non-seeded scaffolds.…”

Section: Tissue Engineering Of the Urinary Bladdermentioning

confidence: 99%

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Adipose-Derived Stem Cells — Are They the Optimal Cell Source for Urinary Tract Regeneration?

Orabi¹,

Goulet²,

Fradette³

et al. 2014

Cells and Biomaterials in Regenerative Medicine

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“…PGA was reported to be associated with excessive lymphocyte infiltration and slow degradation at 8 weeks [16]. ASCs-seeded tissueengineered prepuce scaffolds only enlarged bladder capacity by approximately 1.3-fold at 90 days, with an unsatisfactory number of CD31 + vessels in a rat model [17].…”

Section: Introductionmentioning

confidence: 97%

Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

Xiao¹,

Hu²,

Zhang³

et al. 2018

European Urology Supplements

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Bladder muscular wall regeneration with autologous adipose mesenchymal stem cells on three-dimensional collagen-based tissue-engineered prepuce and biocompatible nanofibrillar scaffold

Cited by 30 publications

References 22 publications

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

A novel technique for simultaneous whole‐body and multi‐organ decellularization: umbilical artery catheterization as a perfusion‐based method in a sheep foetus model

Adipose-Derived Stem Cells — Are They the Optimal Cell Source for Urinary Tract Regeneration?

Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model

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