Bladder cancer diagnosis with a four-miRNA panel in serum

Abstract

Background: Bladder cancer is one of the most prevalent malignancies. Due to the disadvantage of existing bladder cancer diagnostic tools, miRNAs hold promise as new diagnostic markers. Materials & methods: A total of 224 participants were involved in this three-cohort trial. A total of 15 candidate miRNAs were selected, and miRNAs with diagnostic ability were screened out with quantitative reverse transcription PCR. Diagnostic capability was ascertained by the receiver operating characteristic curve and area under the curve. Bioinformatics analysis was constructed for target gene prediction and functional annotation. Results: Six candidate miRNAs showed significantly different expression between bladder cancer patients and normal controls, and the final diagnostic panel comprised miR-181b-5p, miR-183-5p, miR-199-5p and miR-221-3p. Conclusion: This four-miRNA panel could represent a stable biomarker for bladder cancer diagnosis.

Non-coding RNA transcripts, incredible modulators of cisplatin chemo-resistance in bladder cancer through operating a broad spectrum of cellular processes and signaling mechanism

“…miR-6087, miR-6724–5p, miR-3960, miR-1343–5p, miR-1185-1-3p, miR-6831–5p and miR-4695–5p) has been recognized to be beneficial in specific differentiation of BC from non-cancerous conditions and other tumor species [ 275 ]. Furthermore, the combination of miR-181b-5p, miR-183–5p, miR-199–5p and miR-221–3p can be desirably employed to diagnose BC [ 276 ].…”

“…In line with these urinary miRNAs, miR-148b-3p, miR-3187–3p, miR-15b-5p, miR-27a-3p, and miR-30a-5p, which are markedly detectable in serum specimens, are capable biomarkers for a great BC prognosis [ 277 ]. Xie et al [ 278 ] demonstrated that hsa-miR-185–5p, hsa-miR-663a, hsa-miR-30c-5p, hsa-miR-3648, hsa-miR-1270, hsa-miR-200c-3p, and hsa-miR-29c-5p were up-modulated in serum samples obtained from BC patients compared to the control subjects [ 276 , 278 ]. If the corresponding miRNAs become dysregulated, a decrease in the OS of BC patients will then be observed.…”

Non-coding RNA and autophagy: Finding novel ways to improve the diagnostic management of bladder cancer

“…A combination of 7 miRNAs (7-miRNA panel: miR-6087, miR-6724-5p, miR-3960, miR-1343-5p, miR-1185-1-3p, miR-6831-5p and miR-4695-5p) was shown to accurately discriminate bladder cancer from non-cancer and other types of tumors with the high specificity ( Usuba et al, 2019 ). Another study showed that the combination of four miRNAs; miR-181b-5p, miR-183-5p, miR-199-5p and miR-221-3p can be used as a stable biomarker for bladder cancer diagnosis ( Li et al, 2022c ).…”

“…Lian et al found miR-148b-3p, miR-3187-3p, miR-15b-5p, miR-27a-3p, and miR-30a-5p in serum samples could be the potential biomarkers for the prognosis of bladder cancer ( Lian et al, 2018 ). A study by Xie et al identified seven miRNAs were up-regulated in bladder serum cancer samples compared to control samples, including hsa-miR-185-5p, hsa-miR-663a, hsa-miR-30c-5p, hsa-miR-3648, hsa-miR-1270, hsa-miR-200c-3p, and hsa-miR-29c-5p ( Xie et al, 2017b ; Li et al, 2022c ). The dysregulation of these miRNAs correlated with the advanced stage and overall survival in bladder cancer patients.…”

miR-125b-5p, miR-155-3p, and miR-214-5p and Target E2F2 Gene in Oral Squamous Cell Carcinoma

“…In addition, miRNAs as tumour suppressors (oncosuppressor miRs) can also participate in the downregulation of oncogenes [ 16 , 17 ]. Some studies examine the role of miRNA in various cancers, such as pancreatic [ 18 ], colon [ 19 ], prostate [ 20 , 21 ], renal [ 22 ], bladder [ 23 ], breast [ 24 ], cervical [ 25 ], ovarian [ 26 ], lung [ 27 ], and HNSCC [ 28 ]. To date, analyses of the role of miRNAs in HNSCC pathogenesis are focused generally on studies of miRNAs, such as miR-375, miR-1234, miR-103, miR-638, miR-200b-3p, miR-191-5p, miR-24-3p, miR-572, miR-483-5p, miR-20a, miR-22, miR-29a, miR-29b, mir-let-7c, miR-17, miR-374b-5p, miR-425-5p, miR-122, miR-134, miR-184, miR 191, miR-412, miR-512, miR-8392, miR-21, miR-31, miR-155, miR-196a, miR-196b, miR-9, miR-29c, miR-223, miR-187, Let-7a, miR- 27, miR-34, miR-92, miR-124, miR-125a, miR-136, miR-139, miR-145, miR-146a, miR-200, miR-195, and miR-205 [ 29 , 30 , 31 , 32 , 33 ].…”