Bladder Acellular Matrix Conjugated with Basic Fibroblast Growth Factor for Bladder Regeneration

Co-delivery of VEGF and bFGF via a PLGA nanoparticle-modified BAM for effective contracture inhibition of regenerated bladder tissue in rabbits

“…More recently, a multitude of carrier strategies, e.g., direct absorption, lyophilization, chemical crosslinking and nanoparticle-embedded structures, have been developed 16 17 18 19 20 21 22 . Using these carriers, the release period of bioactive components can be prolonged for a few days, but this extended period still cannot meet the physiological needs of the bladder tissue regeneration process, which usually lasts for weeks or months.…”

“…Some reports have used a series of indirect urodynamic parameters, such as bladder capacity and bladder compliance, to evaluate graft contracture 17 21 22 . In our study, we used the preserved graft area to evaluate the contracture rate of the regenerated bladder after augmentation; this evaluation method is more objective and accurate.…”

“…One strategy to improve the outcome of bladder tissue regeneration is to incorporate a BAM with biologically active growth factors 15 . Many incorporation methods, such as injection, rehydration, and incubation, have been demonstrated to enhance the ability of the BAM to mediate bladder tissue regeneration in the short term (2 or 3 weeks) 16 17 18 19 20 21 22 . However, the introduced growth factors may not have a noticeable effect for a long time.…”

Bioengineering Kidneys for Transplantation

“…Soluble factors such as growth factors and cytokines can also be delivered in a controlled spatial and temporal fashion depending on how they are incorporated into the scaffold 62 . Conjugating a rodent bladder acellular matrix with basic fibroblast growth factor accelerated the cellularization and vascularization of the bladder after implantation 63 . Finally, fluid flow through the glomerulus and filtration across tubules generate forces that are essential for proper kidney cell function.…”

Biofabrication and biomaterials for urinary tract reconstruction

“…Loading scaffolds with bioactive agents creates a microenvironment simulating ECM. This environment allows cells to grow, interact, and differentiate into complex tissues, specifically urothelial, smooth muscle, vascular, and nerve tissues 74,76. Bioactive molecules can be incorporated into the bulk of the polymer either during synthesis or by surface conjugation.…”