Black spots concentrate oncogenic asbestos fibers in the parietal pleura. Thoracoscopic and mineralogic study.

Boutin, Claude; Dumortier, Pascal; Rey, F.; Viallat, J. R.; Vuyst, Paul De

doi:10.1164/ajrccm.153.1.8542156

Cited by 165 publications

(135 citation statements)

References 19 publications

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“…The pathways through which fibers reach the pleura, and the populations and sizes of fibers that are responsible for this proliferation, are presently unknown. Our finding of site-specific mesothelial cell proliferation in the rodent parietal pleura strongly supports the recent observation of Boutin and colleagues (16), who found that asbestos fibers accumulate in specific sites in the human parietal pleura associated with lymphatic drainage.…”

Section: Discussionsupporting

confidence: 92%

Comparison of pleural responses of rats and hamsters to subchronic inhalation of refractory ceramic fibers.

Everitt

Gelzleichter

Bermudez

et al. 1997

Environ Health Perspect

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In the present subchronic study, we compared pleural inflammation, visceral pleural collagen deposition, and visceral and parietal pleural mesothelial cell proliferation in rats and hamsters identically exposed to a kaolin-based refractory ceramic fiber, (RCF)-1 by nose-only inhalation exposure, and correlated the results to translocation of fibers to the pleural cavity. Fischer 344 rats and Syrian golden hamsters were exposed to 650 fibers/cc of RCF-1, for 4 hr/day, 5 days/week for 12 weeks. Following 4 and 12 weeks of exposure, and after a 12-week recovery period, pleural lavage fluid was analyzed for cytologic and biochemical evidence of inflammation. Visceral and parietal pleural mesothelial cell proliferation was assessed by immunocytochemical detection of bromodeoxyuridine incorporation. Pleural collagen was quantitated using morphometric analysis of lung sections stained with Sirius Red. Fiber-exposed rats and hamsters had qualitatively similar pleural inflammation at each time point. Mesothelial cell proliferation was more pronounced in hamsters than in rats at each time point and at each site. In both species, the mesothelial cell labeling index was highest in the parietal pleural mesothelial cells lining the surface of the diaphragm at each time point. Hamsters but not rats had significantly elevated collagen in the visceral pleura at the 12-week postexposure time point. Fibers were found in the pleural cavities of both species at each time point. These fibers were generally short and thin. These results suggest that mesothelial cell proliferation and fibroproliferative changes in the pleura of rodents following short-term inhalation exposure are associated with fiber translocation to the pleura and may be predictive of chronic pleural disease outcomes following long-term exposure.

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Section: Discussionsupporting

confidence: 92%

Comparison of pleural responses of rats and hamsters to subchronic inhalation of refractory ceramic fibers.

Everitt

Gelzleichter

Bermudez

et al. 1997

Environ Health Perspect

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“…Dodson et al 4) , found asbestos fibers (predominantly chrysotile) in pleural hyaline plaque taken from 8 cases of shipyard workers. Boutin et al 24) , also found highly concentrated asbestos fibers in black spots (glomerate lymphatic capillaries stained dark due to anthracotic pigmentation) in the parietal pleura. They said that amphibole outnumbered chrysotile in the black spots.…”

Section: Commentsmentioning

confidence: 90%

Asbestos Tissue Burden Study on Human Malignant Mesothelioma.

Suzuki

Yuen

2001

INDUSTRIAL HEALTH

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Asbestos fibers in the lung and mesothelial tissues (mesotheliomatous tissue and hyaline plaque) taken from 151 human malignant mesothelioma cases were identified and characterized by high resolution analytical electron microscopy. Asbestos fibers were present in almost all of the lung tissue as well as in the mesothelial tissue. The most common asbestos types seen in the lung were an admixture of chrysotile with amphiboles followed by amphiboles alone and chrysotile alone. The majority of asbestos types seen in the mesothelial tissues were chrysotile alone, followed by chrysotile plus amphibole and amphibole alone. A disproportion of asbestos types between the lung and mesothelial tissues was frequently observed. The most common pattern of the disproportion was chrysotile plus amphibole(s) in the lung and chrysotile only in the mesothelial tissues, followed by amphibole(s) in the lung and chrysotile only in the mesothelial tissues. Such a disproportion was considered to have been caused by chrysotile fiber's strong capacity to translocate from the lung to mesothelial tissues. The number of asbestos fibers in the lung was 456.4 × × × × × 10 6 fibers/dry gram in maximum, 0.08 × × × × × 10 6 fibers/dry gram in minimum and 105 × × × × × 10 6 fibers/dry gram on average; in the mesothelial tissues it was 240.0 × × × × × 10 6 fibers/dry gram in maximum, 0.03 × × × × × 10 6 fibers/dry gram in minimum and 49.84 × × × × × 10 6 fibers/dry gram on average. These numbers were greater than those seen in the general population. The majority of asbestos fibers detected in the lung and mesothelial tissues were shorter than 5 µm in length. Asbestos fibers fit to Stanton's hypothetical dimensions (≥8.0 µm in length and ≤0.25 µm in diameter) were only 4.0%, since the majority of these fibers were shorter (<8 µm) and thinner (<0.25 µm) fibers. We concluded that such short, thin asbestos fibers should not be excluded from those contributing to the induction of human malignant mesothelioma. The present study supports that chrysotile asbestos can induce human malignant mesothelioma, since, in some of the mesothelioma cases, asbestos fibers detected in both the lung and mesothelial tissues, or lung tissue alone or mesothelial tissues alone were exclusively chrysotile fibers.

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“…Asbestos fibers have been identified in the pleura by autopsy, chrysotile being the predominant asbestos form found in pleural plaques [51] and pleural/mesothelial tissues in general [46,52]. In a singular contradicting report amphibole fibers outnumbered chrysotile ones in anthracotic "black spots" in the parietal pleura sampled during thoracoscopy from all 14 studied individuals [53]. Chrysotile may undergo not only longitudinal splitting but also breakage into shorter fibers, which may be cleared more readily [18]; however, short chrysotile fibers were reported to prevail in the pleura [48,52].…”

Section: Page -02mentioning

confidence: 93%

Asbestos-Related Research: First Objectivity then Conclusions

2015

J Environ Stud

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Black spots concentrate oncogenic asbestos fibers in the parietal pleura. Thoracoscopic and mineralogic study.

Cited by 165 publications

References 19 publications

Comparison of pleural responses of rats and hamsters to subchronic inhalation of refractory ceramic fibers.

Comparison of pleural responses of rats and hamsters to subchronic inhalation of refractory ceramic fibers.

Asbestos Tissue Burden Study on Human Malignant Mesothelioma.

Asbestos-Related Research: First Objectivity then Conclusions

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