Black Seed (Nigella sativa): A Favourable Alternative Therapy for Inflammatory and Immune System Disorders

Ojueromi, Opeyemi Oluwafemi; Oboh, Ganiyu; Ademosun, Ayokunle O.

doi:10.1007/s10787-022-01035-6

Cited by 12 publications

(7 citation statements)

References 151 publications

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“…Furthermore, NSE markedly inhibited inflammation and hepatocyte apoptosis via PPARγ influenced modulation of JAK2/STAT3 and Bcl-2/Bax/Caspase-9 signaling pathways, suggesting a possible hepatoprotective role of NSE. NSE, rich in polyphenols such as quercetin, gallic acid, chlorogenic acid, m-coumeric acid, ferulic acid and sinapic acid, has been recognized for antioxidative [37,38], analgesic [39], anti-inflammatory, immunomodulatory [16,19] and anticancer [40] activities. Therefore, NSE was tested for its hepatoprotective effectiveness in this study.…”

Section: Discussionmentioning

confidence: 99%

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Nigella sativa L. Attenuates Oxidative Stress, Inflammation and Apoptosis in Concanavalin A-induced Acute Immunological Liver Damage in Mice

Mushtaq,

Aslam,

Faisal

et al. 2024

Braz. arch. biol. technol.

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Liver's contribution to innate immunity is eminent. However, uncontrolled inflammatory conditions predispose the liver to immune-mediated injury. Nigella sativa L. is traditionally implicated in infectious, inflammatory, metabolic and hepatorenal complications. This study aimed to evaluate the protective role of N. sativa seed extract (NSE) against concanavalin A (ConA)-induced acute immunological liver injury in mice. In vitro, NSE was subjected to quantitative phytochemical characterization and 1,1-diphenyl-2-picryhydrazyl (DPPH) analysis. In vivo, male Balb/c mice were pretreated with NSE (100, 200 and 400 mg/kg/day, p.o.) and pioglitazone (5 mg/kg/day, p.o.) for seven consecutive days. A single dose of ConA (12 mg/kg, i.v.) was injected and samples were collected for biochemical, histopathological and qRT-PCR analyses after 8 h of ConA injection. In vitro analysis showed considerable quantities of polyphenols and significant DPPH scavenging ability of NSE. In mice, ConA resulted in a significant (p<0.05) increase in liver injury markers (ALT, AST, ALP and TBil) and hepatic oxidative stress (SOD, CAT and MDA). Also, a substantial elevation of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) in liver tissues was noticed. Furthermore, ConA HIGHLIGHTS• Nigella sativa seed extract (NSE) possess significant polyphenol contents and antioxidant potential.• NSE attenuated concanavalin A-induced liver injury and oxidative stress.• NSE suppressed liver inflammation through modulating PPARγ/JAK2/STAT3 pathway.• NSE inhibited hepatocyte apoptosis via downregulating Bcl-2/Bax/Caspase-9 pathway.

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Section: Discussionmentioning

confidence: 99%

“…Nigella sativa L., known as 'Black seeds' in English and 'Kalonji' in Urdu, belongs to the Ranunculaceae family. The plant is widely cultivated in Pakistan, India, Saudi Arabia, Iran, Egypt, Syria, Turkey, Greece and Albania [16]. Traditionally, it is used to cure inflammation, rheumatism, bronchitis, eczema and hypertension [17].…”

Section: Introductionmentioning

confidence: 99%

Nigella sativa L. Attenuates Oxidative Stress, Inflammation and Apoptosis in Concanavalin A-induced Acute Immunological Liver Damage in Mice

Mushtaq,

Aslam,

Faisal

et al. 2024

Braz. arch. biol. technol.

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“…It is also used to reduce the intensity of diseases like Asthma, inflammation, arthritis, gastro, and liver problems. It has been labelled as a pan-assay interference chemical that binds to several proteins without preference [ 58 ]. TQ has also shown potential for usage in cancer-associated thrombosis as it has a very nominal effect on normal blood coagulation.…”

Section: Natural Compounds As Cytochrome Inhibitorsmentioning

confidence: 99%

Evidence-based capacity of natural cytochrome enzyme inhibitors to increase the effectivity of antineoplastic drugs

Manthalkar

AJAZUDDIN²,

Bhattacharya

2022

Discov Onc

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Cytochrome (CYP) enzymes catalyze the metabolism of numerous exogenous and endogenous substrates in cancer therapy leading to significant drug interactions due to their metabolizing effect. CYP enzymes play an important role in the metabolism of essential anticancer medications. They are shown to be overexpressed in tumor cells at numerous locations in the body. This overexpression could be a result of lifestyle factors, presence of hereditary variants of CYP (Bio individuality) and multi-drug resistance. This finding has sparked an interest in using CYP inhibitors to lower their metabolizing activity as a result facilitating anti-cancer medications to have a therapeutic impact. As a result of the cytotoxic nature of synthetic enzyme inhibitors and the increased prevalence of herbal medication, natural CYP inhibitors have been identified as an excellent way to inhibit overexpression sighting their tendency to show less cytotoxicity, lesser adverse drug reactions and enhanced bioavailability. Nonetheless, their effect of lowering the hindrance caused in chemotherapy due to CYP enzymes remains unexploited to its fullest. It has been observed that there is a substantial decrease in first pass metabolism and increase in intestinal absorption of chemotherapeutic drugs like paclitaxel when administered along with flavonoids which help suppress certain specific cytochrome enzymes which play a role in paclitaxel metabolism. This review elaborates on the role and scope of phytochemicals in primary, secondary and tertiary care and how targeted prevention of cancer could be a breakthrough in the field of chemotherapy and oncology. This opens up a whole new area of research for delivery of these natural inhibitors along with anticancer drugs with the help of liposomes, micelles, nanoparticles, the usage of liquid biopsy analysis, artificial intelligence in medicine, risk assessment tools, multi-omics and multi-parametric analysis. Further, the site of action, mechanisms, metabolites involved, experimental models, doses and observations of two natural compounds, quercetin & thymoquinone, and two plant extracts, liquorice & garlic on CYP enzymes have been summarized. Graphical Abstract

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“…The increase in the number of lymphocytes indicates that black cumin has and impact as an immunostimulant can increase the proliferation of lymphocyte cells, which has an impact on increasing immunity system (Hidayati et al, 2019). Immunostimulant mechanism in increasing the number of lymphocytes by stimulating macrophages to produce interleukins, then producing more lymphocytes and plasma cells (antibodies) when attacked by bacterial pathogenic agents (Ojueromi et al, 2022).…”

Section: Leukocyte Differential Lymphocytesmentioning

confidence: 99%

Utilizing of black cumin (Nigella sativa) flour to increase the immunity system of tilapia (Oreochromis niloticus) against Aeromonas hydrophila bacteria attack

Silviana

Rosidah

Pamungkas

et al. 2022

Jurnal Akuakultur Indonesia

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ABSTRACT This study aims to determine dose of black cumin flour in fish feed diets which can improve the disease resistance of tilapia against the attack of A. hydrophila. This study was used tilapia juvenile with a size of 7 – 8 cm. This study was conducted from March – May 2021 and the experimental method is completely randomized design (CRD) with 5 treatments and 3 replications. The treatment used were addition of black cumin flour into fish feed with doses 0 (A), 20 (B), 35 (C), 50 (D), and 65 (E) g/Kg feed. The treatments fed by supplemental diets contained black cumin flour was conducted for 14 days, then a challenge test was carried out with the infection of A. hydrophila bacteria by immersion for14 days. A. hydrophila bacteria used to infect fish had a density of 108 CFU/mL. The Parameters used were total leukocyte, hematocrit, total leukocyte differential, clinical symptoms, survival rate and water quality. The result showed that black cumin flour was effective to increase the immunity system of tilapia. The dose of 50 g/Kg feed was effective showed increase the highest percentage increase in white blood cells of 64.56%, compared to treatments B, C, and E which showed an increase in white blood cells of 47.40%, 48.82% and 1.2%, respectively. Treatment A decreased the percentage of white blood cells by 19.52%. The 50 g dose of black cumin showed the highest percentage value of hematocrit and leukocyte differential in tilapia fry, respectively 34%, lymphocytes 84.3%, monocytes 6%, and neutrophils 9.7% with the fastest recovery period (4 days) and after the challenge test resulted in the highest survival rate of 95%. Keywords: Aeromonas hydrophila, tilapia juvenile, black cumin, immune system ABSTRAK Penelitian ini bertujuan untuk menentukan dosis tepung jintan hitam dalam formula pakan ikan yang dapat meningkatkan ketahanan tubuh ikan nila terhadap serangan bakteri A. hydrophila. Penelitian ini menggunakan benih ikan nila dengan ukuran 7-8 cm. Penelitian ini dilaksanakan pada bulan Maret – Mei 2021 dan metode eksperimen yang digunakan adalah model Rancangan Acak Lengkap dengan lima perlakuan dan tiga ulangan. Perlakuan yang digunakan adalah penambahan tepung jintan hitam pada pakan dengan dosis 0 (A), 20 (B), 35 (C) 50 (D) dan 65 (E) g/Kg pakan. Pemeliharaan ikan uji yang diberikan perlakuan yang mengandung tepung jintan hitam dilakukan selama 14 hari, setelah itu dilanjutkan uji tantang menggunakan bakteri A. hydrophila dengan metode immersi selama 14 hari. Bakteri A. hydrophila yang digunakan untuk menginfeksi ikan memiliki kepadatan 108 CFU/mL. Parameter yang digunakan yaitu leukosit, hematokrit, differensial leukosit, gejala klinis, kelangsungan hidup dan kualitas air. Hasil penelitian memperlihatkan tepung jintan hitam dapat meningkatkan ketahanan tubuh ikan nila. Dosis jintan hitam 50 g per 1 kg pakan paling efektif, terlihat dari persentase peningkatan sel darah putih tertinggi sebesar 64.56%, dibandingkan perlakuan B, C, dan E yang masing masing menunjukan peningkatan sel darah putih sebesar 47.40%, 48.82% dan 1.2%. Perlakuan A mengalami penurunan persentase sel darah putih sebesar 19.52%. Dosis jintan hitam 50 g menunjukkan nilai persentase hematokrit dan differensial leukosit yang paling tinggi pada benih ikan nila, masing-masing sebesar 34%, limfosit 84.3%, monosit 6%, dan neutrofil 9.7% dengan masa pemulihan tercepat (4 hari) dan setelah di uji tantang menghasilkan kelangsungan hidup tertinggi yaitu sebesar 95%. Kata kunci: Aeromonas hydrophila, benih ikan nila, jintan hitam, sistem imu

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Black Seed (Nigella sativa): A Favourable Alternative Therapy for Inflammatory and Immune System Disorders

Cited by 12 publications

References 151 publications

Nigella sativa L. Attenuates Oxidative Stress, Inflammation and Apoptosis in Concanavalin A-induced Acute Immunological Liver Damage in Mice

Nigella sativa L. Attenuates Oxidative Stress, Inflammation and Apoptosis in Concanavalin A-induced Acute Immunological Liver Damage in Mice

Evidence-based capacity of natural cytochrome enzyme inhibitors to increase the effectivity of antineoplastic drugs

Utilizing of black cumin (Nigella sativa) flour to increase the immunity system of tilapia (Oreochromis niloticus) against Aeromonas hydrophila bacteria attack

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