Black rockfish C-type lectin, SsCTL4: A pattern recognition receptor that promotes bactericidal activity and virus escape from host immune defense

Du, Xiang; Wang, Guanghua; Su, Yan-li; Zhang, Min; Hu, Yonghua

doi:10.1016/j.fsi.2018.05.033

Cited by 33 publications

(19 citation statements)

References 51 publications

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“…Tissue distribution analysis showed that ToCTL1 and ToCTL2 were expressed in all studied tissues of healthy obscure puffer, which was consistent with other reported CTLs (13)(14)(15)21). The mRNA transcription of CLEC17A gene from Totoaba macdonaldi showed a constitutive expression in all examined tissues, and its expression levels were the highest in the head-kidney, liver, spleen, and skin (21).…”

Section: Discussionsupporting

confidence: 88%

“…OnCL11X1 from O. niloticus was highly expressed in the liver and widely exhibited in the kidney, intestines, and spleen (14). In S. schlegelii, SsCTL4 expression was distributed in the brain, muscle, kidney, spleen, intestine, heart, gills, and liver with an increasing order (15). CaNTC from C. auratus was the most abundant in liver, followed by the head-kidney and spleen (13).…”

Section: Discussionmentioning

confidence: 98%

“…Sequence comparison showed that the predicted ToCTL1 and ToCTL2 proteins primarily contained two regions: a single transmembrane domain and one typical CRD domain. These structural features were found conserved in the following fish species: T. macdonaldi (21), S. schlegelii (15), Lates calcarifer (22), Plecoglossus altivelis (23), and Salmo salar (1). However, the CTL receptors can be also soluble proteins (24).…”

Section: Discussionmentioning

confidence: 99%

“…In large yellow croaker Larimichthys crocea, LcNTC expression was highly expressed in the liver, spleen, and head-kidney following infection with V. parahaemolyticus (29). The expression levels of SsCTL4 in the head-kidney, liver, and spleen of Black rockfish were remarkably enhanced after being challenged with Vibrio anguillarum (15). As a ubiquitous bacterium, A. hydrophila is considered one of the most important pathogens for human and lower vertebrates, including fish and amphibians (30,31).…”

Section: Discussionmentioning

confidence: 99%

“…Recombinant OnCL11X1 protein agglutinates the two aforementioned bacteria in vitro and promotes phagocytosis by macrophages (14). A CTL family with four member SsCTL4 from the teleost fish black rockfish (Sebastes schlegelii) serves as a pattern recognition receptor that not only possesses the inhibitory effect on Edwardsiella tarda infection but also serves as targets for virus (infectious spleen and kidney necrosis virus) manipulation of the host defense system (15). However, little is known about the roles of CTLs in the innate immunity of pufferfish.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Functional Analysis of Two Transmembrane C-Type Lectins in Obscure Puffer (Takifugu obscurus)

Huang

Shi

et al. 2020

Front. Immunol.

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C-type lectins (CTLs) have received widespread attention in animal immune responses. In the present study, two CTLs (ToCTL1 and ToCTL2) were identified from obscure puffer Takifugu obscurus. The open reading frames of ToCTL1 and ToCTL2 were 687 and 1,380 bp, respectively. The predicted ToCTL1 and ToCTL2 proteins contained a single transmembrane region and one typical carbohydrate recognition domain (CRD). Quantitative real-time polymerase chain reaction detected ToCTL1 and ToCTL2 transcripts in all examined tissues, with high levels in the intestine and kidney, and their expression levels were remarkably altered upon Vibrio harveyi and Aeromonas hydrophila infection. The recombinant proteins ToCTL1-CRD and ToCTL2-CRD agglutinated the Gram-negative and Gram-positive bacteria in a Ca 2+-dependent manner. rToCTL1-CRD and rToCTL2-CRD exhibited evident binding activities against seven kinds of bacteria and polysaccharides (lipopolysaccharide and peptidoglycan) in a Ca 2+-independent manner. Moreover, rToCTL1-CRD and rToCTL2-CRD could inhibit the growth of four types of bacteria in vitro. These findings collectively demonstrated that ToCTL1 and ToCTL2 could be involved in host defense against bacterial infection in T. obscurus.

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