Black-pigmented anaerobic bacteria associated with ovine periodontitis

Borsanelli, Ana Carolina; Gaetti‐Jardim, Elerson; Schweitzer, Christiane Marie; Viora, Lorenzo; Busin, Valentina; Riggio, Marcello P.; Dutra, Iveraldo S.

doi:10.1016/j.vetmic.2017.03.032

Cited by 34 publications

(46 citation statements)

References 31 publications

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“…Black-pigmented bacteria of the genera Porphyromonas and Prevotella are recognised pathogens in human and animal periodontitis. Different species of both genera have been identified in dogs (Hardham et al, 2005;Nishiyama et al, 2007;Riggio et al, 2011), cats (Booij-Vrieling et al, 2010) and sheep with periodontitis (McCourtie et al, 1989, Duncan et al, 2003Riggio et al, 2013;Borsanelli et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Microbiomes associated with bovine periodontitis and oral health

Borsanelli

Lappin

Viora

et al. 2018

Veterinary Microbiology

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Periodontitis is an infectious polymicrobial, immuno-inflammatory disease of multifactorial aetiology that has an impact on the health, production and welfare of ruminants. The objective of the present study was to determine the microbial profiles present in the gingival sulcus of cattle considered periodontally healthy and in the periodontal pocket of animals with periodontitis lesions using high-throughput bacterial 16S rRNA gene sequencing. Subgingival biofilm samples were collected from 40 cattle with periodontitis and 38 periodontally healthy animals. In total, 1923 OTUs were identified and classified into 395 genera or higher taxa. Microbial profiles in health differed significantly from periodontitis in their composition (p < 0.0001, F = 5.30; PERMANOVA) but no statistically significant differences were observed in the diversity of healthy and periodontitis microbiomes. The most prevalent taxa in health were Pseudomonas, Burkholderia and Actinobacteria, whereas in disease these were Prevotella, Fusobacterium and Porphyromonas. The most discriminative taxa in health were Gastranaerophilales, Planifilum and Burkholderia, and in disease these were Elusimicrobia, Synergistes and Propionivibrio. In conclusion, statistically significant difference exists between the microbiome in bovine oral health and periodontitis, with populations showing 72.6% dissimilarity. The diversity of the bacteria found in health and periodontitis were similar and bacteria recognised as periodontal pathogens showed increased abundance in disease. In this context, the main components of bacterial homeostasis in the biofilm of healthy sites and of dysbiosis in periodontal lesions provide unprecedented indicators for the evolution of knowledge about bovine periodontitis.

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Section: Discussionmentioning

confidence: 99%

Microbiomes associated with bovine periodontitis and oral health

Borsanelli

Lappin

Viora

et al. 2018

Veterinary Microbiology

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“…It is a polymicrobial infectious disease that damages the supporting tissue of the teeth and causes occlusion change, tooth loss, and difficulty in rumination. Its origin is multifactorial, and it has to do with the bacterial flora of the mouth, the immune response of the individual, as well as with certain metabolic and environmental factors [2,[15][16][17][18]. The prevalence is usually high in herds.…”

Section: Introductionmentioning

confidence: 99%

Management of Risk Factors Associated with Chronic Oral Lesions in Sheep

Lacasta

González

Ferrer

et al. 2020

Animals

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Oral disorders constitute a significant cause of weight loss in sheep. In a study of disorders of the oral cavity of 36,033 sheep from 60 meat sheep flocks in Spain, we looked for management risk factors associated with chronic oral lesions. Mandibular and maxillary disorders were assessed as an external manifestation of oral lesions by palpation, searching for tissue swellings, fistulae, or open wounds. The prevalence of flocks containing sheep with jaw disorders was 98.3%, with an average individual prevalence of 5.5%. The majority of lesions were located in in the mid-region of the mandible, and the most relevant risk factor was increasing age. Use of acidic diets based on silage and inclusion of hard foods, such as maize straw or hay, was associated with the occurrence of jaw inflammation. It was concluded that hard diets containing plant material with edges and sharp areas, plus acidic foods including silage, are more likely to cause lesions of the gingiva, enabling entry of pathogens to the oral mucosa that eventually establish locally in bony tissues, usually as mandibular osteomyelitis. It was also observed that mineral supplementation appears to prevent the occurrence of these lesions.

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“…In the present study, it was possible to verify the presence of both species in the periodontal lesions of sheep, but only P. nigrescens showed significant association with the disease. P. nigrescens has been identified in bovines and sheep with periodontitis (Borsanelli et al 2015a(Borsanelli et al , 2017, and is frequently detected in the subgingival sulcus biofilm of healthy human patients with periodontitis (Mayanagi et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Riggio et al (2013) evaluated the presence of specific bacterial species in sheep samples with and without periodontitis and emphasized that there were clear differences in the composition of the microbiota between ill and healthy animals. Specimens of two genera of periodontopathogens, Porphyromonas and Prevotella, belonging to the Socransky Red and Orange Complexes, showed close association with lesions of ovine periodontitis (Borsanelli et al 2017). Likewise, some species of spirochetes of genus Treponema have also been identified in sheep with periodontitis (Borsanelli et al 2016).…”

Section: Introductionmentioning

confidence: 91%

Subgingival bacterial microbiota associated with ovine periodontitis

et al. 2019

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Periodontitis is an inflammatory response in a susceptible host caused by complex microbiota, predominantly composed of Gram-negative anaerobic bacteria. Aiming to characterize the subgingival bacterial microbiota associated with ovine periodontitis, polymerase chain reaction (PCR) was performed in subgingival periodontal pocket samples of 14 sheep with severe periodontitis and in subgingival sulcus biofilm of 14 periodontally healthy sheep in search mainly of Gram-negative and Gram-positive microorganisms considered important periodontopathogens. The most prevalent bacteria in the sheep with periodontal lesions were Tannerella forsythia (78.6%), Treponema denticola (78.6%), Fusobacterium nucleatum (64.3%), and Porphyromonas gingivalis (50%), whereas in the healthy sheep, F. nucleatum (42.8%) was the most often detected bacterium. Statistically significant differences were observed for Campylobacter rectus, Enterococcus faecium, Prevotella nigrescens, T. forsythia, and T. denticola (p<0.05) in the sheep with periodontitis in the comparison between groups. Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, and Porphyromonas gulae were not detected in any of the samples analyzed. In conclusion, C. rectus, E. faecium, P. nigrescens, T. forsythia, and T. denticola were associated with severe lesions caused by ovine periodontitis, and F. nucleatum was the most prevalent microorganism in the subgengival sulcus biofilm of healthy sheep.

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Black-pigmented anaerobic bacteria associated with ovine periodontitis

Cited by 34 publications

References 31 publications

Microbiomes associated with bovine periodontitis and oral health

Microbiomes associated with bovine periodontitis and oral health

Management of Risk Factors Associated with Chronic Oral Lesions in Sheep

Subgingival bacterial microbiota associated with ovine periodontitis

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