Black mulberry (Morus nigra L.) polysaccharide ameliorates palmitate-induced lipotoxicity in hepatocytes by activating Nrf2 signaling pathway

Chen, Wei; Yang, Lu; Hu, Dongwen; Mo, Jianling; Ni, Jingdan

doi:10.1016/j.ijbiomac.2021.01.059

Cited by 20 publications

(17 citation statements)

References 45 publications

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“…Caspases regulate apoptosis mainly through the mitochondrial pathway and death receptor pathway, both of which involve caspase-3 [ 87 ]. In the death receptor pathway, the DNA cleavage factor is activated via caspase-3, leading to nuclear DNA cleavage and eventually cell death [ 88 , 89 ]. Therefore, by measuring the relative expression of caspase-3 in IPEC-1 cells, it is possible to confirm the protective impact of RGPs against H 2 O 2 -induced oxidative damage.…”

Section: Resultsmentioning

confidence: 99%

Structural Characteristics of Rehmannia glutinosa Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells

Ren

Gao

et al. 2022

Foods

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Polysaccharide decolorization is a key determinant of polysaccharide structure. In this study, two purified Rehmannia glutinosa polysaccharides, RGP−1−A and RGP−2−A, were obtained after decolorization using the AB-8 macroporous resin and H2O2, respectively. RGP−1−A (molecular weight (Mw) = 18,964 Da) and RGP−2−A (Mw = 3305 Da) were acidic and neutral heteropolysaccharides, respectively, and were both polycrystalline in structure. FTIR analysis revealed that RGP−1−A was a sulfate polysaccharide, while RGP−2−A had no sulfate group. Experiments on IPEC-1 cells showed that RGPs alleviated oxidative stress by regulating the Nrf2/Keap1 pathway. These findings were confirmed by the upregulation of Nrf2, NQO1, and HO-1; the subsequent increase in the levels of antioxidant indicators (SOD, LDH, CAT, and MDA); and the restoration of mitochondrial membrane potential. Overall, the antioxidant capacity of RGP−1−A was significantly higher than that of RGP−2−A. These results suggest that RGPs may be a potential natural antioxidant and could be developed into functional foods.

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Section: Resultsmentioning

confidence: 99%

Structural Characteristics of Rehmannia glutinosa Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells

Ren

Gao

et al. 2022

Foods

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“…Mulberry ( Morus atropurpurea ) fruits were regarded to have beneficial biofunctions [ 40 ]. For example, the polysaccharides from black mulberry could reduce ROS formation, improve mitochondrial function, and activate the Nrf2 signaling pathway [ 41 ], and the extracts from mulberry fruits showed the anti-cancer effect on the HepG2 cells because they were capable of suppressing cell growth and inducing cell apoptosis [ 42 ]. Yam ( Dioscorea Opposita Thunb.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Immuno-Modulatory Potentials of Purslane (Portulaca oleracea L.) Polysaccharides with a Chemical Selenylation

Lin

Guan

et al. 2021

Foods

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The soluble polysaccharides from a non-conventional and edible plant purslane (Portulaca oleracea L.), namely PSPO, were prepared by the water extraction and ethanol precipitation methods in this study. The obtained PSPO were selenylated using the Na2SeO3-HNO3 method to successfully prepare two selenylated products, namely SePSPO-1 and SePSPO-2, with different selenylation extents. The assay results confirmed that SePSPO-1 and SePSPO-2 had respective Se contents of 753.8 and 1325.1 mg/kg, while PSPO only contained Se element about 80.6 mg/kg. The results demonstrated that SePSPO-1 and SePSPO-2 had higher immune modulation than PSPO (p < 0.05), when using the two immune cells (murine splenocytes and RAW 264.7 macrophages) as two cell models. Specifically, SePSPO-1 and SePSPO-2 were more active than PSPO in the macrophages, resulting in higher cell proliferation, greater macrophage phagocytosis, and higher secretion of the immune-related three cytokines, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β. Meanwhile, SePSPO-1 and SePSPO-2 were more potent than PSPO in the concanavalin A- or lipopolysaccharide-stimulated splenocytes in cell proliferation, or more able than PSPO in the splenocytes to promote interferon-γ secretion but suppress IL-4 secretion, or more capable of enhancing the ratio of T-helper (CD4+) cells to T-cytotoxic (CD8+) cells for the T lymphocytes than PSPO. Overall, the higher selenylation extent of the selenylated PSPO mostly caused higher immune modulation in the model cells, while a higher polysaccharide dose consistently led to the greater regulation effect. Thus, it is concluded that the employed chemical selenylation could be used in the chemical modification of purslane or other plant polysaccharides, when aiming to endow the polysaccharides with higher immuno-modulatory effect on the two immune cells.

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“…The polysaccharides consisted of Fuc, Rib, Man, Glc, Ara, Gal, Rha, Xyl, GlcA, GalA, mannuronic acid (ManA), GlcN, and/or GalN ( 30 , 75 – 78 ). Moreover, Man, Glc, Ara, Gal, and Rha were five monosaccharide types widely founded in the polysaccharides ( 45 , 46 , 76 , 79 – 86 ).…”

Section: Structural Features Of Nps Having Regulation On Nrf2 Antioxi...mentioning

confidence: 99%

“…Ara f , Ara p , Rha p , Gal p , Glc p , Man p , Xyl p , GalA p , and GlcA p sugar residues have been determined in the polysaccharides from woody plants. Ara f residues exhibited as T-Ara f -(1→, →2)-Ara f -(1→, →4)-Ara f -(1→, →5)-Ara f -(1→, →2,5)-Ara f -(1→ and →3,5)-Ara f -(1→ ( 30 , 46 , 73 , 74 ). Ara p residues reflected as T-Ara p -(1→, →4)-Ara p -(1→, →3,4)-Ara p -(1→ and →2,3,4)-Ara p -(1→ ( 73 , 74 , 87 – 90 ).…”

Section: Structural Features Of Nps Having Regulation On Nrf2 Antioxi...mentioning

confidence: 99%

“…Glc p residues revealed as T-Glc p -(1→, →2)-Glc p -(1→, →4)-Glc p -(1→, →6)-Glc p -(1→, →3,4)-Glc p -(1→ and →4,6)-Glc p -(1→ ( 49 , 50 , 93 , 94 ). Man p residues behaved as T-Man p -(1→, →2)-Man p -(1→, →4)-Man p -(1→, →6)-Man p -(1→ and →3,6)-Man p -(1→ ( 46 , 73 , 74 , 92 ). Xyl p residues manifested as T-Xyl p -(1→, →3)-Xyl p -(1→ and →4)-Xyl p -(1→ ( 73 , 74 , 87 , 88 ).…”

Section: Structural Features Of Nps Having Regulation On Nrf2 Antioxi...mentioning

confidence: 99%

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Advances in health-promoting effects of natural polysaccharides: Regulation on Nrf2 antioxidant pathway

Luo

Shen

et al. 2023

Front. Nutr.

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Natural polysaccharides (NPs) possess numerous health-promoting effects, such as liver protection, kidney protection, lung protection, neuroprotection, cardioprotection, gastrointestinal protection, anti-oxidation, anti-diabetic, and anti-aging. Nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant pathway is an important endogenous antioxidant pathway, which plays crucial roles in maintaining human health as its protection against oxidative stress. Accumulating evidence suggested that Nrf2 antioxidant pathway might be one of key regulatory targets for the health-promoting effects of NPs. However, the information concerning regulation of NPs on Nrf2 antioxidant pathway is scattered, and NPs show different regulatory behaviors in their different health-promoting processes. Therefore, in this article, structural features of NPs having regulation on Nrf2 antioxidant pathway are overviewed. Moreover, regulatory effects of NPs on this pathway for health-promoting effects are summarized. Furthermore, structure-activity relationship of NPs for health-promoting effects by regulating the pathway is preliminarily discussed. Otherwise, the prospects on future work for regulation of NPs on this pathway are proposed. This review is beneficial to well-understanding of underlying mechanisms for health-promoting effects of NPs from the view angle of Nrf2 antioxidant pathway, and provides a theoretical basis for the development and utilization of NPs in promoting human health.

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Black mulberry (Morus nigra L.) polysaccharide ameliorates palmitate-induced lipotoxicity in hepatocytes by activating Nrf2 signaling pathway

Cited by 20 publications

References 45 publications

Structural Characteristics of Rehmannia glutinosa Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells

Structural Characteristics of Rehmannia glutinosa Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells

In Vitro Immuno-Modulatory Potentials of Purslane (Portulaca oleracea L.) Polysaccharides with a Chemical Selenylation

Advances in health-promoting effects of natural polysaccharides: Regulation on Nrf2 antioxidant pathway

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