Black Grape Juice Protects Spleen from Lipid Oxidation Induced by Gamma Radiation in Rats

Abstract

This study evaluated the effects of black grape juice (BGJ) against gamma radiationinduced damage in rats. Rats were exposed (PI) or not (PNI) to radiation and BGJ (2 mL/Kg of body weight) was administrated to irradiated animals (SI) and to nonirradiated animals (SNI). Blood was collected at different time points after irradiation for blood cells counts. The spleen of the animals was removed for weight, lipid peroxidation (LPO) and antioxidant defenses estimation. Blood cell count decreased, whereas LPO and catalase activity increased in spleen along with a decrease in the organ weight after irradiation. Despite the lack of protective effect on hematological parameters and catalase activity, BGJ attenuated LPO induced by gamma radiation. This protective effect seems to be related to the phenolic compounds found in BGJ, such as resveratrol, quercetin and rutin. We demonstrated that BGJ plays a beneficial role in damage caused by gamma radiation by preventing spleen LPO. PRACTICAL APPLICATIONSGrape products, such as black grape juice (BGJ), and their polyphenol compounds have several properties that can be useful in prevention and treatment of many conditions. One of these properties is the antioxidant capacity, which makes of phenolic compounds, such as resveratrol, quercetin and rutin, good exogenous scavengers of free radicals. The present study demonstrates that BGJ exhibits a radiomodifier potential against gamma radiation by exerting antioxidant effects and, hence, it can be used as a valuable agent for managing radiation exposure. bs_bs_banner Journal of Food Biochemistry

The effect of naringenin on the role of nuclear factor (erythroid‐derived 2)‐like2 (Nrf2) and haem oxygenase 1 (HO‐1) in reducing the risk of oxidative stress‐related radiotoxicity in the spleen of rats

“…Furthermore, γ‐radiation exposure resulted in a significant reduction in the first‐line defense enzymatic antioxidants such as SOD, CAT, and Gpx and the decrease of these enzymes might result from the interaction of the excess ROS with enzymes' molecules leading ultimately to complete or partial inactivation of the enzymes . This is consistent with previous reports of increased oxidative stress in rats after 6 Gy of γ‐irradiation …”

“…37 This is consistent with previous reports of increased oxidative stress in rats after 6 Gy of γ-irradiation. 38 Nrf2, which is a transcription factor responsible for the regulation of cellular redox balance and protective antioxidant. Nrf2 is kept in the cytoplasm by Kelch like-ECH-associated protein 1 (Keap1) and Cullin 3, which degrade Nrf2 by ubiquitination.…”

Effect of Black Grape Juice against Heart Damage from Acute Gamma TBI in Rats

“…Ionizing radiation exposure caused by environmental, industrial, medical and accidental reasons can make the organism susceptible to various harmful conditions [30]. The whole body exposure a high dose of radiation also called the acute radiation syndrome (ARS) or radiation sickness, and might cause alterations in the hematopoietic [16], cardiovascular [31] and digestive systems besides disturbance in biochemical parameters [32]. Various studies were designed to prospect positive radiomodifiers which might reduce the incidence of injuries related to ARS, among them, medicinal plants, vitamins and, minerals.…”

“…Compounds capable of preventing or mitigating damage as a result of exposure to radiation are called radiomodifiers and have been studied [12,13,14,15,16]. These substances possess the property of reducing the effect of ionizing radiation considered due to damage from free radicals and ROS formed once radiation interacts with water, especially by catalyzing the ROS and, NRS generated in the radiation-tissue interaction.…”

Evaluation of Salicin Isolated from Salix subserrata as a Radioprotector against Gamma Irradiation Induced Ultrastructural and Electrophoretic Changes in Spleen Tissue in Rats

“…On the other hand, Rebouch and Seim (1998) 59 and Ibrahim et al The study showed that the decrease in CAT and GPx activity could be attributed to the uncontrolled production of ROS and accumulation of H2O2 whereby oxidative damage to enzymes can cause a modification of their activity 83,84 . Salicin and salicylic acid belonged to the phenolic compounds which showed antioxidant activity due to their ability to scavenge free radicals 88 .…”