Black ginger extract and its active compound, 5,7-dimethoxyflavone, increase intestinal drug absorption via efflux drug transporter inhibitions

Boonnop, Rattiporn; Meetam, Paranee; Siangjong, Lawan; Tuchinda, Patoomrat; Thongphasuk, Piyanut; Soodvilai, Sunhapas; Soodvilai, Sirima

doi:10.1016/j.dmpk.2023.100500

Cited by 2 publications

(7 citation statements)

References 24 publications

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“…Caco-2 monolayers are a suitable tool for studying drug-natural product interactions in vitro. 17,[98][99][100][101] However, it must be kept in mind that a natural product may change the pH of the test solution and affect the permeabilities of the drugs. 17 93 The use of precolumn is also recommended for samples analyzed with high-performance liquid chromatography.…”

Section: Evaluation Of the Effects Of Natural Extracts On The Permeab...mentioning

confidence: 99%

“…Baker) is widely used herb in Asia and Thailand. 98 It is used wide variety of indications such as to stimulate sexual performance, relieve depression, 128 and anti-obesity. [128][129][130] It is also reported to possess neuroprotective, anti-inflammatory, anticancer, antiallergic, and cardioprotective properties.…”

Section: Echinaceamentioning

confidence: 99%

“…34,[131][132][133][134][135][136] It contains secondary metabolites, especially flavonoids and flavonoid glycosides, among which 5,7-dimethoxyflavone (DMF) is the major constituent. 98,137 A Caco-2 cell study showed that black ginger 10 µg/ml ethanol extract and 5,7-DMF 5 µM were able to increase digoxin, estrone stulfate, and paclitaxel absorption. 98 The studies suggested that the observed increase was due to the inhibition of P-gp and BCRP.…”

Section: Echinaceamentioning

confidence: 99%

“…98,137 A Caco-2 cell study showed that black ginger 10 µg/ml ethanol extract and 5,7-DMF 5 µM were able to increase digoxin, estrone stulfate, and paclitaxel absorption. 98 The studies suggested that the observed increase was due to the inhibition of P-gp and BCRP. The results were confirmed with Madin-Darby canine kidney cell line II (isolated from a high passage parental cell line) (MDCKII) cell lines.…”

Section: Echinaceamentioning

confidence: 99%

“…Caco-2 monolayers are a suitable tool for studying drug–natural product interactions in vitro . 17,98-101 However, it must be kept in mind that a natural product may change the pH of the test solution and affect the permeabilities of the drugs. 17 Acidic compounds are transported more effectively in acidic environments, and basic compounds behave the opposite way.…”

Section: Evaluation Of the Effects Of Natural Extracts On The Permeab...mentioning

confidence: 99%

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Natural Product – Drug Interactions and Their Evaluation Using a Caco-2 Cell Culture Model

Galkin,

Tammela

2023

Natural Product Communications

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Natural products, such as herbal medicinal products, food supplements, and functional food, are widely used to support well-being and for promoting health. In general, the effects of using these products are desired and beneficial, but unexpected adverse effects might also occur, especially when natural products are used with medication. Consumers do not often even know that they are exposed to bioactive compounds that might interact with the body and have effects on their well-being. One of the objectives of the World Health Organization is to promote herb–drug interaction monitoring, and it is important to know how marketed preparations and compounds from common foods interact when they are absorbed. In this review, we describe how a Caco-2 cell absorption model has been used to study how natural products, such as flaxseed, rapeseed, purple loosestrife, pine, echinacea, certain berries and herbs, anthranoid laxatives, and traditional medicinal plants, affect the absorption of co-administered drugs. We discuss the types of interactions and adverse effects that might occur and their possible reasons. Overall, we conclude that the Caco-2 cell absorption model is a useful tool for studying the absorption of natural products with drugs; and that to enable the safe use of natural products with medicines, concomitant use should be studied.

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Section: Evaluation Of the Effects Of Natural Extracts On The Permeab...mentioning

confidence: 99%

Section: Echinaceamentioning

confidence: 99%

Section: Echinaceamentioning

confidence: 99%

Section: Echinaceamentioning

confidence: 99%

Section: Evaluation Of the Effects Of Natural Extracts On The Permeab...mentioning

confidence: 99%

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Natural Product – Drug Interactions and Their Evaluation Using a Caco-2 Cell Culture Model

Galkin,

Tammela

2023

Natural Product Communications

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Novel Screening System for Biliary Excretion of Drugs Using Human Cholangiocyte Organoid Monolayers with Directional Drug Transport

Mizoi,

Okada,

Mashimo

et al. 2024

Biological & Pharmaceutical Bulletin

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It has recently been reported that cholangiocyte organoids can be established from primary human hepatocytes. The purpose of this study was to culture the organoids in monolayers on inserts to investigate the biliary excretory capacity of drugs. Cholangiocyte organoids prepared from hepatocytes had significantly higher mRNA expression of CK19, a bile duct epithelial marker, compared to hepatocytes. The organoids also expressed mRNA for efflux transporters involved in biliary excretion of drugs, P-glycoprotein (P-gp), multidrug resistance-associated protein 2 (MRP2), and breast cancer resistance protein (BCRP). The subcellular localization of each protein was observed. These results suggest that the membrane-cultured cholangiocyte organoids are oriented with the upper side being the apical membrane side (A side, bile duct lumen side) and the lower side being the basolateral membrane side (B side, hepatocyte side), and that each efflux transporter is localized to the apical membrane side. Transport studies showed that the permeation rate from the B side to the A side was faster than from the A side to the B side for the substrates of each efflux transporter, but this directionality disappeared in the presence of inhibitor of each transporter. In conclusion, the cholangiocyte organoid monolayer system has the potential to quantitatively evaluate the biliary excretion of drugs. The results of the present study represent an unprecedented system using human cholangiocyte organoids, which may be useful as a screening model to directly quantify the contribution of biliary excretion to the clearance of drugs.

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Black ginger extract and its active compound, 5,7-dimethoxyflavone, increase intestinal drug absorption via efflux drug transporter inhibitions

Cited by 2 publications

References 24 publications

Natural Product – Drug Interactions and Their Evaluation Using a Caco-2 Cell Culture Model

Natural Product – Drug Interactions and Their Evaluation Using a Caco-2 Cell Culture Model

Novel Screening System for Biliary Excretion of Drugs Using Human Cholangiocyte Organoid Monolayers with Directional Drug Transport

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