Black cardamom (Amomum subulatum Roxb.) fruit extracts exhibit apoptotic activity against lung cancer cells

Makhija, Pooja; Handral, Harish K; Mahadevan, Gomathi; Kathuria, Himanshu; Sethi, Gautam; Grobben, Bert

doi:10.1016/j.jep.2021.114953

Cited by 14 publications

(11 citation statements)

References 38 publications

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“…A recent study using the methanolic extract of fruits of Amomum subulatum showed that the anti‐inflammatory mechanism exerted by this herb may be mediated by the HO‐1 system along with the traditional cyclooxygenase pathway, setting a new pace in drug research and development in this field (Bairwa et al., 2011; Drishya et al., 2021a; Gautam et al., 2016). A significant anticancer activity was also demonstrated by the fruit by upregulating the expresson of p53, downregulating Bcl2, and overexpression of cleaved PARP‐1 (Poly (ADP‐ribose) polymerase‐1 in different cells lines (Makhija et al., 2022; Ramakrishnan, 2021). In addition, other biological parameters such as antinociceptive, cardioadaptagenic effects have also been demonstrated by the extracts and essential oils of Amomumsubulatum .…”

Section: Discussionmentioning

confidence: 99%

“…IC 50 values of the hexane and ethyl acetate extracts were found to be 622 ± 115 μg/mL and 778 ± 225 μg/mL for the MCF‐7 cell line and 500 ± 67 μg/mL and 724 ± 223 μg/mL for HeLa cell lines, respectively Makhija et al. (2022) analyzed the cytotoxic effects of Amomum subulatum's fruit extracts (hexane, dichloromethane, tetrahydrofuran, methanol, and water extract) for their cytotoxicity against various cell lines (MCF‐7, MDA‐MB‐231, A549, NCI‐H1299, HepG2, and HCCLM3). The findings of MTT assay suggest that cell viability of all four extracts at 100 μg/mL dose was the lowest in lung cancer cells (A549 and H1299).…”

Section: Pharmacological Implications Of Amomum Subulatum and Its Con...mentioning

confidence: 95%

“…The finding suggests that DE administration at a dose of 150 μg/mL displayed morphological alterations such as shape distortion, rounding, surface detachment, generation of ROS (0‐150 μg/mL), and superoxide radicals (150 μg/mL) including cell‐cycle arrest in the sub‐G 1 phase (150 μg/mL for 24 and 48 hours). Furthermore, overexpression of cleaved poly (ADP‐ribose) on A549 cells expressed the failure of DNA repairing machinery, which eventually contributed to apoptosis (Makhija et al., 2022). Administration of ethanolic extract of the whole Amomum subulatum (62.5 μg/mL) fruit also manifested apoptotic morphological changes in HeLa cell lines as expressed by RT‐PCR techniques, which include upregulation of the p53 gene and downregulation of the Bcl2 gene (Ramakrishnan, 2021).…”

Section: Pharmacological Implications Of Amomum Subulatum and Its Con...mentioning

confidence: 99%

“…It is also well known for its flavor and fragrance‐enhancing properties along with antiseptic, antimicrobial, anticancer, anti‐inflammatory, and antioxidant properties (Cai et al., 2021). A very recent investigation has highlighted the potential of dichloromethane extract of Amomum subulatum for its apoptotic activity against lung cancer cells (Makhija et al., 2022). Amomum subulatum's essential oil and various fractions have also shown lipid‐lowering (Bairwa et al., 2011), gastroprotective (Jafri et al., 2001), and antimicrobial (Tijjani et al, 2012) properties.…”

Section: Introductionmentioning

confidence: 99%

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Prophylactic and therapeutic potential of active phytoconstituents from Amomum subulatum Roxb

et al. 2022

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Amomum subulatum is a popular aromatic and medicinal spice crop being used for centuries to treat a variety of health ailments. The ethnopharmacological use of Amomum subulatum has been described in various medicinal systems including Ayurveda, Unani, and Chinese for its therapeutic properties in mitigating gastrointestinal disorders, lung congestions, respiratory ailments, jaundice, tuberculosis, hyperlipidemia, inflammation, ischemic heart diseases, etc. Literature search using Google Scholar, PubMed, NCBI, Elsevier, Wiley online Library, Springer, Taylor and Francis, Web of Science, Science Direct, Scopus databases was restricted to English-language articles. Significant information on pharmacological properties of Amomum subulatum, including antioxidant (ROS/RNS pathways), anti-inflammatory (TNF-α, IL-1, IL-6, COX-2, NF-кB, HO-1, arachidonic acid pathways), antinephrotoxic (urea, creatinine, Cystatin-C), antihepatotoxic (SGOT, SGPT, and ALP), antidiabetic, etc., was collected for the review. The pharmacological properties of this traditional herb may be attributed to the plethora of secondary metabolites present in it. Preliminary phytochemical screening reported the abundance of flavonoids, terpenoids, steroids, vitamins, minerals, fatty acid esters, etc. 1,8-cineole (60%-80% of total volatile oil), α-terpineol, limonene, α-terpinyl acetate, etc., are the notable phytochemicals reported from Amomum subulatum. However, detailed insights into the molecular mechanistic pathways mediating the beneficial role of this traditional herb on cell/animal models and large-scale clinical trials are lacking.In the present review, we provide a comprehensive description of the current knowledge on Amomum subulatum, including its phytoconstituents, pharmacological uses, and toxicities, its implication in host health effects, including in vitro/in vivo efficacy, and the potential of these emerging species to suggest areas for future research.

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Section: Discussionmentioning

confidence: 99%

Section: Pharmacological Implications Of Amomum Subulatum and Its Con...mentioning

confidence: 95%

Section: Pharmacological Implications Of Amomum Subulatum and Its Con...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prophylactic and therapeutic potential of active phytoconstituents from Amomum subulatum Roxb

et al. 2022

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“…The fruit extracts also exhibited anticancer activity in mice through the control of cytokines that cause inflammation as well as the NF-κB signaling ( Sudarsanan et al., 2021 ). A recent study discovered that the dichloromethane extract of A. subulatum had an apoptotic effect against lung cancer cells ( Makhija et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Insight into the phytochemical profile and antimicrobial activities of Amomum subulatum and Amomum xanthioides: an in vitro and in silico study

et al. 2023

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IntroductionMedicinal plants have been considered as potential source of therapeutics or as starting materials in drugs formulation.MethodsThe current study aims to shed light on the therapeutic potential of the Amomum subulatom and Amomum xanthioides Fruits by analyzing the phytochemical composition of their seeds and fruits using gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) techniques to determine the presence of bioactive components such as flavonoids, phenols, vitamins, steroids, and essential oils.Results and DiscussionThe protein content is usually higher than the total lipids in both species except the fruit of A. subulatum which contain more lipids than proteins. The total protein contents for A. subulatum were 235.03 ± 21.49 and 227.49 ± 25.82 mg/g dry weight while for A. xanthioides were 201.9 ± 37.79 and 294.99 ± 37.93 mg/g dry weight for seeds and fruit, respectively. The Carvacrol levels in A. subulatum is 20 times higher than that in A. xanthioides. Lower levels of α-Thujene, Phyllanderenes, Ascaridole, and Pinocarvone were also observed in both species. According to DPPH (2,2-diphenylpicrylhydrazyl) assay, seed the extract of A. subulatum exhibited the highest antioxidant activity (78.26±9.27 %) followed by the seed extract of A. xanthioides (68.21±2.56 %). Similarly, FRAP (Ferric Reducing Antioxidant Power) assay showed that the highest antioxidant activity was exhibited by the seed extract of the two species; 20.14±1.11 and 21.18±1.04 µmol trolox g−1 DW for A. subulatum and A. xanthioides, respectively. In terms of anti-lipid peroxidation, relatively higher values were obtained for the fruit extract of A. subulatum (6.08±0.35) and the seed extract of A. xanthioides (6.11±0.55). Ethanolic seed extracts of A. subulatum had the highest efficiency against four Gram-negative bacterial species which causes serious human diseases, namely Pseudomonas aeruginosa, Proteus vulgaris, Enterobacter aerogenes, and Salmonella typhimurium. In addition, P. aeruginosa was also inhibited by the fruit extract of both A. subulatum and A. xanthioides. For the seed extract of A. xanthioides, large inhibition zones were formed against P. vulgaris and the fungus Candida albicans. Finally, we have in silico explored the mode of action of these plants by performing detailed molecular modeling studies and showed that the antimicrobial activities of these plants could be attributed to the high binding affinity of their bioactive compounds to bind to the active sites of the sterol 14-alpha demethylase and the transcriptional regulator MvfR.ConclusionThese findings demonstrate the two species extracts possess high biological activities and therapeutical values, which increases their potential value in a number of therapeutic applications.

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Chemistry and Functionality of Black Cardamom (Amomum subulatum)

Sharma,

Hussein,

Cheikhyoussef

et al. 2023

Cardamom (Elettaria Cardamomum): Production, Processing and Properties

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Black cardamom (Amomum subulatum Roxb.) fruit extracts exhibit apoptotic activity against lung cancer cells

Cited by 14 publications

References 38 publications

Prophylactic and therapeutic potential of active phytoconstituents from Amomum subulatum Roxb

Prophylactic and therapeutic potential of active phytoconstituents from Amomum subulatum Roxb

Insight into the phytochemical profile and antimicrobial activities of Amomum subulatum and Amomum xanthioides: an in vitro and in silico study

Chemistry and Functionality of Black Cardamom (Amomum subulatum)

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