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Oliveira, Rosário; Marques, Fernanda; Azeredo, Joana

doi:10.1023/a:1008954301470

Cited by 31 publications

(4 citation statements)

References 3 publications

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“…Although TCA precipitation is usually highly efficient and widely used to remove peptides from polysaccharides [ 52 , 53 , 54 ], the efficiency reported here is lower than previously reported for polysaccharidic polymers from heterotrophic bacterial and plant sources [ 55 , 56 ], but in the same range as the ones reported for other cyanobacterial polymers [ 57 , 58 ]. In some cases, such as for polysaccharides from Spirulina platensis , higher TCA concentrations had to be used to achieve a precipitation efficiency similar to the one reported here (~41% less peptides) [ 57 ].…”

Section: Resultscontrasting

confidence: 59%

Assessing the Antitumor Potential of Variants of the Extracellular Carbohydrate Polymer from Synechocystis ΔsigF Mutant

et al. 2023

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Cancer is a leading cause of death worldwide with a huge societal and economic impact. Clinically effective and less expensive anticancer agents derived from natural sources can help to overcome limitations and negative side effects of chemotherapy and radiotherapy. Previously, we showed that the extracellular carbohydrate polymer of a Synechocystis ΔsigF overproducing mutant displayed a strong antitumor activity towards several human tumor cell lines, by inducing high levels of apoptosis through p53 and caspase-3 activation. Here, the ΔsigF polymer was manipulated to obtain variants that were tested in a human melanoma (Mewo) cell line. Our results demonstrated that high molecular mass fractions were important for the polymer bioactivity, and that the reduction of the peptide content generated a variant with enhanced in vitro antitumor activity. This variant, and the original ΔsigF polymer, were further tested in vivo using the chick chorioallantoic membrane (CAM) assay. Both polymers significantly decreased xenografted CAM tumor growth and affected tumor morphology, by promoting less compact tumors, validating their antitumor potential in vivo. This work contributes with strategies for the design and testing tailored cyanobacterial extracellular polymers and further strengths the relevance of evaluating this type of polymers for biotechnological/biomedical applications.

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Section: Resultscontrasting

confidence: 59%

Assessing the Antitumor Potential of Variants of the Extracellular Carbohydrate Polymer from Synechocystis ΔsigF Mutant

et al. 2023

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“…TFPs were extracted using boiling tap water (1:60, w/v) for 6 h. The extract solution was then centrifuged (3,000×g, 5 min) and the supernatant was concentrated to a quarter of the original weight under vacuum at 70°C. Subsequently, deproteination was performed by adjusting the solution pH to pH 4.0 with citric acid and kept at 4°C for 4 days to enable the complete precipitation of the proteins by the isoelectric precipitation method [Oliveira et al, 1999]. The supernatant was collected and three equivalent volumes of 95% ethanol were added to precipitate polysaccharides at 4°C overnight.…”

Section: Methodsmentioning

confidence: 99%

Effects of <i>Tremella fuciformis</i>-Derived Polysaccharides with Different Molecular Weight on D-Galactose-Induced Aging of Mice

Luo¹,

Liu²,

Guan³

et al. 2023

Pol. J. Food Nutr. Sci.

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The structure-bioactivity relationship of Tremella fuciformis polysaccharides (TFPs) in anti-aging in vivo is rarely reported. In the present study, a purified TFP, named HM, mainly composed of mannose, fucose, xylose, and glucose in a molar ratio of 4.14:0.98:0.81:0.62, was obtained from the fruiting body of T. fuciformis. Subsequently, two differentially degraded TFPs, named MM and LM, respectively, were prepared by a combined method of ultrasonic irradiation (US) and H 2 O 2 treatment. Their structural properties, scavenging activities against free radicals in vitro, and anti-aging effects on d-galactose-induced aging of mice were determined. The average molecular weight of HM, MM, and LM was 58.3×10 6 , 4.68×10 6 , and 3.14×10 5 Da, respectively. All three TFPs were devoid of triple helix conformation and exhibited concentration-and molecular weight--dependent scavenging activity against radicals. The TFPs markedly relieved skin aging, effectively attenuated oxidative stress, and significantly decreased inflammation in d-galactose-induced aging mice. MM exhibited the best anti-aging effect among the TFPs. Additionally, TFPs partially restored the alterations in pH and the total content of short-chain fatty acids (SCFAs) in the colon but exhibited various impacts on the content of the individual SCFAs. These findings would provide rational guidance for a better application of TFPs in anti-aging foods and expand our understanding of the structure-function relationship of mushroom polysaccharides.

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“…Further details on polysaccharide purification are described in detail in the following review (Shi, 2016). The removal of proteins is also possible through chemical methods combined with centrifugation, such as the Sevag method taking advantage of chloroform for protein denaturation (He et al, 2012;Zhu et al, 2016), the trichlorotrifluorethane method and the trichloroacetic method (Chambi et al, 2021;Oliveira et al, 1999;Pintado et al, 2020) leading to protein precipitation. It can also be achieved through enzymatic methods, where proteases digest or hydrolyze the proteins in solution (Wugeditsch et al, 1999;K.…”

Section: Bacterial Eps Productionmentioning

confidence: 99%

The production and application of bacterial exopolysaccharides as biomaterials for bone regeneration

Bagnol

Grijpma

Eglin

et al. 2022

Carbohydrate Polymers

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Untitled

Cited by 31 publications

References 3 publications

Assessing the Antitumor Potential of Variants of the Extracellular Carbohydrate Polymer from Synechocystis ΔsigF Mutant

Assessing the Antitumor Potential of Variants of the Extracellular Carbohydrate Polymer from Synechocystis ΔsigF Mutant

Effects of <i>Tremella fuciformis</i>-Derived Polysaccharides with Different Molecular Weight on D-Galactose-Induced Aging of Mice

The production and application of bacterial exopolysaccharides as biomaterials for bone regeneration

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