BL153 Partially Prevents High-Fat Diet Induced Liver Damage Probably via Inhibition of Lipid Accumulation, Inflammation, and Oxidative Stress

Abstract

The present study was to investigate whether a magnolia extract, named BL153, can prevent obesity-induced liver damage and identify the possible protective mechanism. To this end, obese mice were induced by feeding with high fat diet (HFD, 60% kcal as fat) and the age-matched control mice were fed with control diet (10% kcal as fat) for 6 months. Simultaneously these mice were treated with or without BL153 daily at 3 dose levels (2.5, 5, and 10 mg/kg) by gavage. HFD feeding significantly increased the body weight and the liver weight. Administration of BL153 significantly reduced the liver weight but without effects on body weight. As a critical step of the development of NAFLD, hepatic fibrosis was induced in the mice fed with HFD, shown by upregulating the expression of connective tissue growth factor and transforming growth factor beta 1, which were significantly attenuated by BL153 in a dose-dependent manner. Mechanism study revealed that BL153 significantly suppressed HFD induced hepatic lipid accumulation and oxidative stress and slightly prevented liver inflammation. These results suggest that HFD induced fibrosis in the liver can be prevented partially by BL153, probably due to reduction of hepatic lipid accumulation, inflammation and oxidative stress.

Lipidomic-based investigation into the regulatory effect of Schisandrin B on palmitic acid level in non-alcoholic steatotic livers

“…To further suggest the anti-hepatic fibrosis activity of SchB, we examined if SchB treatment reduced NASH-related hepatic fibrosis in vivo . Other studies showed that feeding mice HFD for 6 months led to NASH and hepatic fibrosis 30 31 . Here, after feeding mice HFD (Research Diets #D12492) for 6 months, we started intragastric administrating SchB at 50 mg/kg/day or vehicle as control for 14 consecutive days.…”

“…Blood was collected from the orbital vein, adipose and liver tissue samples were dissected for subsequent experiments. For the study of hepatic fibrosis, we used C57/BL mice that had been feeding HFD (Research Diets #D12492) for 6 months as a model 30 31 to investigate if SchB treatment reduced NASH-related hepatic fibrosis. After we fed mice the HFD for 6 months, we started intragastric administrating SchB at 50 mg/kg or vehicle as control for 14 consecutive days.…”

Extracts of Magnolia Species-Induced Prevention of Diabetic Complications: A Brief Review

“…Using mice with HFD, our group firstly investigated the effect of Magnolia extract, BL153 on HFD-induced liver injury, and reported that BL153 treatment significantly suppressed HFD-induced hepatic fibrosis [50]. Further study showed that BL153 significantly inhibited HFD-induced hepatic lipid accumulation and oxidative stress and slightly prevented liver inflammation, which was the underlying mechanism for BL153 against HFD-induced hepatic fibrosis [50].…”

“…Further study showed that BL153 significantly inhibited HFD-induced hepatic lipid accumulation and oxidative stress and slightly prevented liver inflammation, which was the underlying mechanism for BL153 against HFD-induced hepatic fibrosis [50]. In a further study, Lee et al [7] found that combination of honokiol and magnolol inhibited in vitro lipogenesis mediated by liver X receptor α, a nuclear receptor that regulates the metabolism of lipids, in hepatocytes through activation of AMPK, and ameliorated HFD-induced hepatic steatosis and liver dysfunction.…”

4-O′-methylhonokiol protects from alcohol/carbon tetrachloride-induced liver injury in mice

“…Also, honokiol was found to induce apoptotic death in activated rat HSCs suggesting an antifibrotic effect, while there was no such effect on hepatocytes (24). Magnolia officinalis extracts showed beneficial effects on hepatic lipid accumulation, inflammation, oxidative stress, and apoptosis in a HFD-induced obese mouse model (25)(26)(27). M.…”