BK-virus progressive multifocal leukoencephalitis in a patient with systemic lupus erythematosus

Melis, Marta; Badiali, Manuela; Peltz, Teresa; Cossu, Giovanni; Manieli, Cristina; Gianno, Francesca

doi:10.1007/s10072-018-3413-3

Cited by 4 publications

(2 citation statements)

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“…BK virus-associated progressive multifocal leukoencephalitis has previously only been reported in five cases [49]. In the current case, BKPyV was detected in brain tissue, which is considered the best support for diagnosing BKPyV virus encephalitis [49]. The absence of BK viremia in our case suggests localized reactivation of BKPyV in the central nervous sytem (CNS).…”

Section: Technical Performance On Pcr-positive Csf Samplessupporting

confidence: 58%

“…For example, encephalitis caused by BK polyomavirus (BKPyV) has been indeed described in a series of case reports [48]. BK virus-associated progressive multifocal leukoencephalitis has previously only been reported in five cases [49]. In the current case, BKPyV was detected in brain tissue, which is considered the best support for diagnosing BKPyV virus encephalitis [49].…”

Section: Technical Performance On Pcr-positive Csf Samplesmentioning

confidence: 54%

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Improved diagnosis of viral encephalitis in adult and pediatric hematological patients using viral metagenomics

Carbo

Buddingh

Karelioti

et al. 2020

Preprint

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14 15 Word count, abstract: 233 16 Word count, manuscript: 2157 17 Highlights 18 • A metagenomics protocol employing virus capture probes was validated and retrospectively 19 applied to 41 hematological adult and pediatric patients presenting with encephalitis of 20 unknown aetiology 21 • Viral enrichment by capture probes increased sensitivity of viral metagenomics on 22 cerebrospinal fluid samples 100 -10.000 fold, compared to unenriched metagenomic 23 sequencing 24 • In 12% of hematological patients with encephalitis of unknown origin, a virus was detected 25 by viral metagenomics, which was not found by routine diagnostics 26 • Viral metagenomics represents a valuable addition to the diagnostics repertoire for 27 hematological patients with suspected CNS infection 28 3 29 Abstract 30Metagenomic sequencing is a powerful technique that enables detection of the full spectrum of 31 pathogens present in any specimen in a single test. Hence, metagenomics is increasingly being 32 applied for detection of viruses in clinical cases with suspected infections of unknown etiology and a 33 large number of relevant potential causes. This is typically the case in patients presenting with 34 encephalitis, in particular when immunity is impaired by underlying disorders. 35In this study, viral metagenomics has been applied to a cohort of hematological patients with 36 encephalitis of unknown origin. 37Since viral loads in cerebrospinal fluid of patients with encephalitis are generally low, the technical 38 performance of a metagenomic sequencing protocol enriched by capture probes targeting all known 39 vertebrate viral sequences was studied. Subsequently, the optimized viral metagenomics protocol 40 was applied to a cohort of hematological patients with encephalitis of unknown origin. 41 Viral enrichment by capture probes increased the viral sequence read count of metagenomics on 42 cerebrospinal fluid samples 100 -10.000 fold, compared to unenriched metagenomic sequencing. 43In five out of 41 (12%) hematological patients with encephalitis, a virus was detected by viral 44 metagenomics which had not been detected by current routine diagnostics. BK polyomavirus, 45 hepatitis E virus, human herpes virus-6 and Epstein Barr virus were identified by this unbiased 46 metagenomic approach. 47This study demonstrated that hematological patients with encephalitis of unknown origin may 48 benefit from early viral metagenomics testing as a single step approach. 49 50

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Section: Technical Performance On Pcr-positive Csf Samplessupporting

confidence: 58%

Section: Technical Performance On Pcr-positive Csf Samplesmentioning

confidence: 54%