Bivariate and Multivariate Analyses of the Influence of Blood Variables of Patients Submitted to Roux-en-Y Gastric Bypass on the Stability of Erythrocyte Membrane against the Chaotropic Action of Ethanol

Abstract

The stability of the erythrocyte membrane, which is essential for the maintenance of cell functions, occurs in a critical region of fluidity, which depends largely on its composition and the composition and characteristics of the medium. As the composition of the erythrocyte membrane is influenced by several blood variables, the stability of the erythrocyte membrane must have relations with them. The present study aimed to evaluate, by bivariate and multivariate statistical analyses, the correlations and causal relationships between hematologic and biochemical variables and the stability of the erythrocyte membrane against the chaotropic action of ethanol. The validity of this type of analysis depends on the homogeneity of the population and on the variability of the studied parameters, conditions that can be filled by patients who undergo bariatric surgery by the technique of Roux-en-Y gastric bypass since they will suffer feeding restrictions that have great impact on their blood composition. Pathway analysis revealed that an increase in hemoglobin leads to decreased stability of the cell, probably through a process mediated by an increase in mean corpuscular volume. Furthermore, an increase in the mean corpuscular hemoglobin (MCH) leads to an increase in erythrocyte membrane stability, probably because higher values of MCH are associated with smaller quantities of red blood cells and a larger contact area between the cell membrane and ethanol present in the medium.

Influence of age on the correlations of hematological and biochemical variables with the stability of erythrocyte membrane in relation to sodium dodecyl sulfate

“…These statements can be supported by the positive correlation observed between the levels of total and LDL-C and the stability of erythrocyte membrane in a population of obese patients submitted to bariatric surgery. 35 In fact, patients with familial hypercholesterolemia presented increased content of cholesterol in the erythrocyte membrane 36 and mammalians erythrocytes showed decreased osmotic fragility with increase of membrane cholesterol. 37 Furthermore, a decrease in the blood levels of cholesterol is associated to a lower ratio between cholesterol and phospholipids in the membrane, with physicochemical changes as increased membrane fluidity.…”

Relationship between serum cholesterol and indices of erythrocytes and platelets in the US population

“…While it is plausible that disease extremes such as hemolytic anemia may serve to reveal some of the mechanisms that govern blood cell-cholesterol relationships during health, it is likely that additional mechanisms may be at work in large human populations. For example, malnutrition are all positively correlated ( 34 ). It has also been proposed that the stability of erythrocytes may be maximal within an optimal range of membrane fl uidity (and thus membrane cholesterol) ( 17 ).…”

Efeito do exercício físico na estabilidade de membrana de eritrócitos

“…The stability can be defined as the membrane's ability to resist lysis against many harmful agents. Many factors that affect the stability of the membrane are well known; among them are diet, age, temperature, use of drugs and some physiological and pathological conditions (13)(14)(15)(16)(17).…”