Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes

Li, Keyou; Yang, Wenhua; Li, Zhe; Jia, Wangwang; Li, Jiazhou; Zhang, Pengfei; Xiao, Tiancun

doi:10.1016/j.intimp.2016.02.019

Cited by 20 publications

(10 citation statements)

References 44 publications

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“…Green fluorescence signal in ACE group was stronger than control (no treatment) group, and it indicates the upregulation of cleaved caspase‐3 in HaCaT cells after the treatment of ACE. The increment of cleaved caspase‐3 also implies the induction of apoptosis in HaCaT cells (Li et al, ). Finally, the activity of effector caspases (i.e., caspase‐3/7) in HaCaT cells was tested by luminogenic assay (Figure e).…”

Section: Resultsmentioning

confidence: 99%

“…In this experimental method (72 hr incubation in HaCaT cells), the IC 50 value of ACE was 37.5 μg/ml. The experimental methods (i.e., cell seeding density, sample incubation period, and assay type) and the origin of AC may influence on its IC 50 (Li et al, 2016). Finally, the activity of effector caspases (i.e., caspase-3/ 7) in HaCaT cells was tested by luminogenic assay (Figure 2e).…”

Section: Antiproliferation Efficacy Test In Hacat Cellsmentioning

confidence: 99%

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Antiproliferation of keratinocytes and alleviation of psoriasis by the ethanol extract of Artemisia capillaris

Lee

Nam

Hong³

et al. 2018

Phytotherapy Research

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The therapeutic potentials of the ethanol extract of Artemisia capillaris (ACE) for psoriasis were verified in HaCaT cells (as an immortalized human keratinocyte cell line) and imiquimod (IMQ)-induced psoriasis-like mouse models. In HaCaT cells, IC value of ACE was 37.5 μg/ml after incubating for 72 hr. The antiproliferation activity of ACE in HaCaT cells was further verified by apoptosis assays. The percentage of apoptotic population in ACE-treated group was significantly higher than that of control group (p < .05). The result of cell cycle arrest assay also supported the observed antiproliferation efficacy of ACE in HaCaT cells. In IMQ-induced psoriasis-like mouse models, the Psoriasis Area and Severity Index score of ACE (50 mg/ml; ACE50)-treated group was significantly lower than that of IMQ group on Day 4 (p < .05). After topical application of ACE on psoriasis-like lesion for 4 days, the epidermal thickness of (IMQ + ACE50) group was significantly lower than that of IMQ group (p < .05). The expression levels of Ki-67 and intracellular adhesion molecule-1 in excised skin tissues of (IMQ + ACE50) group were also lower than those of IMQ group. All these findings suggest that ACE can be used as a promising antipsoriatic agent.

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Section: Resultsmentioning

confidence: 99%

Section: Antiproliferation Efficacy Test In Hacat Cellsmentioning

confidence: 99%

Antiproliferation of keratinocytes and alleviation of psoriasis by the ethanol extract of Artemisia capillaris

Lee

Nam

Hong³

et al. 2018

Phytotherapy Research

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“…Bitter apricot oil has been shown to induce apoptosis of HaCaT cells through both death receptor and mitochondrial pathways. Apoptosis has been shown to correlate with inhibition of the NF-κB pathway [ 108 ]. It has been suggested that apricot oil may be a potential candidate for psoriasis treatment given its pro-apoptotic effect on human keratinocytes [ 108 ].…”

Section: The Potential Beneficial Effects Of Topical Application Omentioning

confidence: 99%

Anti-Inflammatory and Skin Barrier Repair Effects of Topical Application of Some Plant Oils

Lin

Zhong

Santiago

2017

IJMS

437

300

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Plant oils have been utilized for a variety of purposes throughout history, with their integration into foods, cosmetics, and pharmaceutical products. They are now being increasingly recognized for their effects on both skin diseases and the restoration of cutaneous homeostasis. This article briefly reviews the available data on biological influences of topical skin applications of some plant oils (olive oil, olive pomace oil, sunflower seed oil, coconut oil, safflower seed oil, argan oil, soybean oil, peanut oil, sesame oil, avocado oil, borage oil, jojoba oil, oat oil, pomegranate seed oil, almond oil, bitter apricot oil, rose hip oil, German chamomile oil, and shea butter). Thus, it focuses on the therapeutic benefits of these plant oils according to their anti-inflammatory and antioxidant effects on the skin, promotion of wound healing and repair of skin barrier.

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“…The caspase family plays an important role in apoptosis, and Caspase-3 ultimately carries out apoptosis 40 . The anti-apoptotic Bcl-XL protein in the Bcl-2 family as well as Bad inhibits the activation of Caspase-3, thereby restraining apoptosis 41 .…”

Section: Discussionmentioning

confidence: 99%

iTRAQ-Based Quantitative Proteomic Analysis of the Inhibitory Effects of Polysaccharides from Viscum coloratum (Kom.) Nakai on HepG2 Cells

Chai

Zhao

2017

Sci Rep

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Viscum coloratum (Kom.) Nakai is one of active medicinal plants, and its active components, especially polysaccharides, have been shown to exhibit bioactivity. In this study, we examined the effects of three polysaccharide fractions from Viscum coloratum (Kom.) Nakai on HepG2 cell growth in a dose-dependent manner by using a CCK-8 assay kit. Flow cytometry analysis showed that VCP2 treatment delayed the cell cycle in the G1 phase and induced apoptosis in HepG2 cells, a result possibly due to the increased expression of p21Wafl/Cip1 and Cyclin D and the decreased expression of Cyclin E and CDK4. The increased expression of Bad, Smac and Caspase-3 and the decreased expression of Bcl-XL and XIAP may be some of the reasons for the induction of apoptosis in VCP2-treated HepG2 cells. Through iTRAQ and 2D-LC-MSMS, 113 and 198 differentially expressed proteins were identified in normal and VCP2-treated HepG2 and Caco2 cells. The mRNA and protein levels of Histone H3.1, Cytoskeletal 9 and Vitronectin agreed with iTRAQ proteomic results. GO, pathways and the PPI of differentially expressed proteins were further analyzed. These findings broaden the understanding of the anti-tumor mechanisms of mistletoe polysaccharides and provide new clues for screening proteins that are responsive to polysaccharides.

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Bitter apricot essential oil induces apoptosis of human HaCaT keratinocytes

Cited by 20 publications

References 44 publications

Antiproliferation of keratinocytes and alleviation of psoriasis by the ethanol extract of Artemisia capillaris

Antiproliferation of keratinocytes and alleviation of psoriasis by the ethanol extract of Artemisia capillaris

Anti-Inflammatory and Skin Barrier Repair Effects of Topical Application of Some Plant Oils

iTRAQ-Based Quantitative Proteomic Analysis of the Inhibitory Effects of Polysaccharides from Viscum coloratum (Kom.) Nakai on HepG2 Cells

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