Roughstalk bluegrass {Poa trivialis L.) (RBG) is a weed in many turf settings because it often thins, goes dormant, or dies with increasing summer temperatures. Morphology and physiology of RBG are not well understood. Our objective was to describe morphological differences and physiological changes in shoots and stolons of RBG and creeping bentgrass {Agrostis stolonifera L.) (CBG) during the growing season in Indiana. The study evaluated three grasses ('L93' CBG and 'Laser' and 'Pulsar' RBG) over nine harvests from field plots between April and October of 2008 and 2009. Turf quality and percent cover were consistently higher for CBG compared to RBG. However, mild summer temperatures in 2008 and 2009 likely resulted in minimal separation between RBG cultivars. Stolon production began in May for RBG and CBG after planting in August of the previous year. Specific stolon dry weights (mg cm^^) of RBG doubled compared to L93 during peak summer stress in August. Total nonstructural carbohydrate (TNC) concentration of RBG shoots decreased 26% while TNC in stolons remained unchanged. Additionally, protein and amino acid concentrations increased in stolons and decreased in shoots of RBG during summer stress, further supporting our hypothesis that stolons act as storage organs during high temperatures. Conserving protein and carbohydrates in stolons at the expense of shoots until high temperature stress subsides may be a stress tolerance mechanism of RBG. ).Abbreviations: CBG, creeping bentgrass {Agrostis stolonifera L.); RBC, roughstalk bluegrass {Poa trivialis L.); SSDW, specific stolon dry weight; TNC, total nonstructural carbohydrate; WSC, water-soluble carbohydrate.
R OUGHSTALK BLUEGRASS {Poa trivialis L.) (RBG) and creepingbentgrass {Agrostis stolonifera L.) (CBG) commonly coexist on golf course fairways, resulting in undesirable playing conditions when RBG often thins in late summer. Physiological and morphological characteristics of RBG may affect high temperature stress tolerance, and a better understanding of these diflferences may lead to improved management or control strategies for RBG. Responses of RBG to high temperature include earlier leaf firing, more open stomata, and dramatic increases in respiration relative to photosynthesis compared to other cool-season grasses (Carroll and Welton, 1937;Watschke et al., 1973;Sifers and Beard, 1993;Loveys et al., 2002). Although RBG sensitivity to high temperature stress is documented, physiological changes associated with its sensitivity remain unclear. However, physiological responses of CBG and other cool-season grasses to high temperature stress are better understood. High temperature stress tolerance of cool-season grasses is dependent on availability of energy sources, such as carbohydrates, and the ability to produce functional proteins to maintain metabolic processes (Baker and Jung, 1968;Xu and Huang, 2001). Concentrations of total nonstructural carbohydrates (TNCs), fructan, and glucose in CBG vary