Bisphenols alter thermal responses and performance in zebrafish (Danio rerio)

Abstract

Plastic pollutants are novel environmental stressors that are now persistent components of natural ecosystems. Endocrine disrupting chemicals such as bisphenols that leach out of plastics can modify physiological responses of animals by interfering with hormone signalling. Here, we tested whether three commonly produced bisphenols, bisphenol A (BPA), bisphenol F (BPF) and bisphenol S (BPS), impair thermal acclimation of swimming performance and metabolic enzyme [citrate synthase (CS) and lactate dehydrogenase (LDH)] activities in adult zebrafish (Danio rerio). We found that exposure to 30-μg l−1 BPF and BPS, but not BPA, reduced swimming performance, and no interactions between bisphenol exposure and acclimation (3 weeks to 18°C and 28°C) or acute test (18°C and 28°C) temperatures were found. BPA interacted with acclimation and acute test temperatures to determine CS activity, an indicator of mitochondrial density and aerobic metabolic capacity. BPS reduced CS activity and an interaction (at a one-tailed significance) between acclimation temperature and BPF exposure determined CS activity. LDH activity reflects anaerobic ATP production capacity, and BPA and BPF altered the effects of thermal acclimation and acute test temperatures on LDH activity. Our data show that all bisphenols we tested at ecologically relevant concentrations can disrupt the thermal responses of fish. BPS and BPF are used as environmentally safer alternatives to BPA, but our data show that these bisphenols are also of concern, particularly in thermally variable environments.

Toxicological effect and enzymatic disorder of non-studied emerging contaminants in Artemia salina model

“…Same trend had been reported in zebra fish ( D. rerio ) treated with different bisphenols (BPA, BPS or BPF) at two temperature conditions (20 °C and 28 °C) for 24 h [ 80 ]. Particularly, bisphenol exposure caused some endocrine disruptions and interfering in some processes such as locomotor performance and energy metabolism [ 85 ]. Therefore, the exposure to TBBPA may have altered carbohydrate catabolism and other physiological processes in artemia.…”

Wild Zebrafish Sentinels: Biological Monitoring of Site Differences Using Behavior and Morphology

“…Phenotypic variation across these endpoints in zebrafish has been shown in laboratory settings previously. Zebrafish exposed to plastic pollution [ 96 , 97 ], arsenic [ 98 ], lead [ 99 ], antibiotics [ 100 ], polycyclic aromatic hydrocarbons [ 101 ], organic pollutants [ 102 ], elevated temperatures [ 103 ], or emerging pollutants [ 104 ] may exhibit altered body mass, size, and swimming behavior. Similarly, hypoxia [ 52 , 105 ] and water flow [ 49 , 52 , 70 , 71 ] are associated with changes in zebrafish morphological and behavioural phenotypes.…”

Interactive effects of temperature and bisphenol A on phytoplankton growth and community structure

“…Reaction rate, favorability and spontaneity are dependent on temperature in both abiotic and biotic systems, and most biological rates, including metabolic rates, are modified by environmental temperature ( Brown et al, 2004 ). Temperature influences the rates of BPA uptake, metabolism and excretion with the rates of these processes typically increasing with increasing temperature ( Honkanen and Kukkonen, 2006 ; Wu and Seebacher, 2020 ; Wu and Seebacher, 2021 ). Thus, temperature may have the capacity to influence the way in which BPA affects individuals and the way in which individuals take up environmental BPA.…”

“…However, the relationship between BPA and temperature may not be as clear in all cases and can depend on both past and current thermal environments (e.g. Wu and Seebacher, 2021 ). In addition, temperature synergistically interacts with other environmental contaminants besides BPA, including heavy metals ( Oukarroum et al, 2012 ) and certain herbicides ( Delorenzo et al, 2013 ), to negatively affect phytoplankton.…”