Bisphenol A modifies human spermatozoa motility in vitro

Kotwicka, Małgorzata; Skibinska, Izabela; Piworun, Natalia; Jendraszak, Magdalena; Chmielewska, Małgorzata; Jȩdrzejczak, Piotr

doi:10.20883/jms.2016.5

Cited by 10 publications

(9 citation statements)

References 20 publications

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“…The present study demonstrated that SE concentration-dependently reversed the motility and survival of BPA-treated spermatozoa. Consistent with our results, BPA diminished sperm quality in humans and some animal species (Kotwicka et al, 2016;Knez et al, 2014;Rahman et al, 2017). Rezaeian et al (2016) showed that SE increases the quality of sperm submitted to freezing and thawing procedures.…”

Section: Discussionsupporting

confidence: 90%

Protective effects of selenium on Bisphenol A-induced oxidative stress in mouse testicular mitochondria and sperm motility

Rafiee

Rezaei-Tazangi

Zeidooni

et al. 2021

JBRA

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Section: Discussionsupporting

confidence: 90%

Protective effects of selenium on Bisphenol A-induced oxidative stress in mouse testicular mitochondria and sperm motility

Rafiee

Rezaei-Tazangi

Zeidooni

et al. 2021

JBRA

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“…Our study showed that TAU reversed the viability and motility of the BPA-exposed sperms in a dose-dependent fashion. Previous reports showed that BPA caused a decrease in sperm quality in rodents and humans ( Rahman et al ., 2016 ; Wisniewski et al ., 2015 ; Kotwicka et al ., 2016 ). BPA impaired reproduction and sperm function in zebrafish ( Chen et al ., 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Taurine effects on Bisphenol A-induced oxidative stress in the mouse testicular mitochondria and sperm motility

Rezaei-Tazangi

Zeidooni

Rafiee

et al. 2020

JBRA Assisted Reproduction

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Objectives: This study was performed to investigate the protective effects of taurine (2-aminoethanesulfonic acid, TAU) on oxidative stress in the isolated mouse testicular mitochondria, mitochondrial membrane potential (MMP), viability and motility of the exposed sperms to the BPA. Methods: We treated epididymal spermatozoa obtained from mice and isolated mouse testicular mitochondria with BPA (0.8 mmol/mL) and various doses of TAU (5, 10, 30 and 50 µmol/L). We used the MTT assay and Rhodamine 123 uptake to assess sperm viability and MMP. We assessed the oxidative stress through measuring ROS (reactive oxygen species), MDA (malondialdehyde), GSH (glutathione), and SOD (super-oxide dismutase) levels in the testicular mitochondrial tissue. Results: BPA significantly elevated ROS, MDA and MMP levels, and markedly reduced SOD and GSH levels in the isolated mitochondria. BPA also considerably impaired spermatozoa viability and motility. Pretreatment with 30 and 50 µmol/L of TAU could considerably suppressed mitochondrial oxidative stress, enhanced MMP, and improved sperm motility and viability. Conclusion: TAU may attenuate the BPA-induced mitochondrial toxicity and impaired sperm motility via decreasing oxidative stress.

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“…Only few studies have examined the effect of bisphenols on human sperm cell function. One study showed that very high doses of BPA (≥300 µM) induced mitochondrial dysfunction in human sperm (57), another study showed that BPA at 0.1 nM−1 µM could affect human sperm motility parameters and that BPA at 1 µM could induce a rapid, transient increase in [Ca 2+ ] i in a whole population of observed single human sperm cells (40), whereas BPA at 0.1, 1, and 10 µM did not affect [Ca 2+ ] i in human sperm cells in a large screening of environmental chemicals by Schiffer et al (34). Our results here support the findings by Schiffer et al (34) that BPA at concentrations up to 10 µM do not induce Ca 2+ signals in human sperm cells.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that human CatSper can be promiscuously activated by various signaling molecules (30), steroids (31,32), small molecules (33), and environmental chemicals (34)(35)(36)(37)(38)(39). As only BPA, and none of its structural analogs, has previously been investigated for effects on Ca 2+ signaling in human sperm cells (34,40), we set out to screen BADGE, BPA, and its eight structural analogs BPG, BPAF, BPC, BPB, BPBP, BPE, BPF, BPS for effects on Ca 2+ signaling, and CatSper in human sperm, as well as on human sperm cell function.…”

Section: Introductionmentioning

confidence: 99%

Bisphenol A Diglycidyl Ether (BADGE) and Bisphenol Analogs, but Not Bisphenol A (BPA), Activate the CatSper Ca2+ Channel in Human Sperm

2020

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Aim: Evidence suggests that bisphenol A diglycidyl ether (BADGE), bisphenol A (BPA), and BPA analogs can interfere with human male fertility. However, the effect directly on human sperm function is not known. The CatSper Ca 2+ channel in human sperm controls important sperm functions and is necessary for normal male fertility. Environmental chemicals have been shown to activate CatSper and thereby affect Ca 2+ signaling in human sperm. BPA has previously been investigated for effects on Ca 2+ signaling human sperm, whereas the effects of other BPA analogs are currently unknown. The aim of this study is thus to characterize the effect of BADGE, BPA, and the eight analogs BPG, BPAF, BPC, BPB, BPBP, BPE, BPF, BPS on Ca 2+ signaling, and CatSper in human sperm. Methods: Direct effects of the bisphenols on Ca 2+ signaling in human sperm cells were evaluated using a Ca 2+ fluorimetric assay measuring changes in intracellular Ca 2+. Effects via CatSper were assessed using the specific CatSper inhibitor RU1968. Effects on human sperm function was assessed using an image cytometry-based acrosome reaction assay and the modified Kremer's sperm-mucus penetration assay. Results: At 10 µM the bisphenols BPG, BPAF, BPC, BADGE, BPB, and BPBP induced Ca 2+ signals in human sperm cells, whereas BPE, BPF, BPS, and BPA had no effect. The efficacy of the chemicals at 10 µM is BPG > BPAF > BPC > BADGE > BPB > BPBP. Dose-response relations of BPG, BPAF, BPC, BADGE, BPB, and BPBP yielded EC50-values in the nM-µM range. The induced Ca 2+ signals were almost completely abolished using the CatSper inhibitor RU1968, indicating an effect of the bisphenols on CatSper. All bisphenols, except BPBP, were found to dose-dependently inhibit progesterone-induced Ca 2+ signals, with BPG and BPAF displaying inhibition even in low µM doses. BPG and BPAF were shown to affect human sperm function in a progesterone-like manner. Rehfeld et al. Bisphenols Activate Human CatSper Conclusion: Our results show that the bisphenols BPG, BPAF, BPC, BADGE, BPB, and BPBP can affect Ca 2+ signaling in human sperm cells through activation of CatSper. This could potentially disrupt human sperm function by interfering with normal CatSpersignaling and thus be a contributing factor in human infertility, either alone or in mixtures with other chemicals.

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Bisphenol A modifies human spermatozoa motility in vitro

Cited by 10 publications

References 20 publications

Protective effects of selenium on Bisphenol A-induced oxidative stress in mouse testicular mitochondria and sperm motility

Protective effects of selenium on Bisphenol A-induced oxidative stress in mouse testicular mitochondria and sperm motility

Taurine effects on Bisphenol A-induced oxidative stress in the mouse testicular mitochondria and sperm motility

Bisphenol A Diglycidyl Ether (BADGE) and Bisphenol Analogs, but Not Bisphenol A (BPA), Activate the CatSper Ca2+ Channel in Human Sperm

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