Bisphenol A induced oxidative stress and apoptosis in mice testes: Modulation by selenium

Abstract

Spermatogenesis, a highly coordinated process, is prone to environmental insults which may lead to impaired spermatogenesis or, at worst, infertility. Bisphenol A (BPA) is a well-known global environmental toxicant and a ubiquitous oestrogenic chemical. This study evaluated the role of selenium (0.5 ppm sodium selenite/kg diet) on spermatogenesis after BPA treatment in different groups of male BALB/c mice: control, selenium, BPA and selenium+BPA. Markers of oxidative stress and apoptosis were evaluated in testis after BPA treatment. Significant decrease in sperm concentration and motility and increased reactive oxygen species(ROS) and LPO levels were seen in BPA group. Histopathological changes revealed extensive vacuolisation, lumen devoid of spermatozoa and decreased germ cell count, confirmed by testicular germ cell count studies. TUNEL assay for apoptosis showed increased number of TUNEL-positive germ cells in BPA group with increased percentage apoptotic index. However, in Se+BPA group, histopathological studies revealed systematic array of all germ cells, preserved basement membrane with relatively less vacuolisation, improved sperm parameters and ROS and LPO levels and decreased number of TUNEL-positive germ cells. These results clearly demonstrate the role of selenium in ameliorating oxidative stress and apoptosis induced upon BPA treatment in mice and can be further used as therapeutic target in male infertility.

Alleviating impact of hydroethanolicMurraya koenigiileaves extract on bisphenol A instigated testicular lethality and apoptosis in mice

“…animals. Similar observations were reported by previous studies (Kaur, Saluja, & Bansal, 2018;Sudha, 2011;Yeh et al, 2009) in which severe vacuolization of spermatozoa in seminiferous tubules was observed upon BPA exposure. However, HEMKLE administration to BPA-treated mice maintained the normal germ cells population and less vacuolization in Group IV (HEMKLE + BPA).…”

“…Histological analysis of testes from all the treatment groups revealed that in Group III (BPA) animals, there was severe vacuolization and displacement by some fibrinoid debris in seminiferous tubules when compared to Group I (Control) and Group II (HEMKLE) animals. Similar observations were reported by previous studies (Kaur, Saluja, & Bansal, ; Sudha, ; Yeh et al, ) in which severe vacuolization of spermatozoa in seminiferous tubules was observed upon BPA exposure. However, HEMKLE administration to BPA‐treated mice maintained the normal germ cells population and less vacuolization in Group IV (HEMKLE + BPA).…”

Bisphenols as a Legacy Pollutant, and Their Effects on Organ Vulnerability

“…BPA at low doses decreases the synthesis of estradiol and inhibits the growth of antral follicles isolated from wild-type and Ahr knock-out mice, through partial involvement of the aryl hydrocarbon receptor pathway [32]. BPA exposure results in decreased sperm counts and motility, and increased ROS and lipid peroxidation in mice testes [25] (Table 3).…”

Studies on the phytomodulatory potential of fenugreek ( Trigonella foenum‐graecum ) on bisphenol‐A induced testicular damage in mice

“…Bisphenol A (BPA) is a synthetic oestrogen and an environmental toxicant which is present in innumerable consumer products and considered as highest utilising industrial chemicals worldwide (Alboghobeish et al, 2019). Humans and wildlife are widely and critically exposed to BPA due to its extensive use for the production of water pipes, drinking containers, dental monomers, medical equipments, etc (Kaur, Saluja, & Bansal, 2018). Worldwide 400 million tonnes of plastics produces annually and roughly 3.5 million tons was manufactured in 2008 (Goncalves, Semprebon, Biazi, Mantovani, & Fernandes, 2018).…”

“…Due to advancement in industrial and manufacturing activities, BPA is becoming ubiquitous in our living environment and have raised various public health concerns (Wu et al, 2018). Animal-based studies have reported that BPA exposure exerts undesirable effects on male reproductive system by altering the expression of genes associated with spermatogenesis and endocrine disruption (Kaur et al, 2018). Another study by Shirani, Alizadeh, Mahdavinia, and Dehghani (2019), showed a marked reduction in the levels of reduced glutathione and catalase whereas elevation in mitochondrial reactive oxygen species (ROS) and lipid peroxidation (LPO) levels along with mitochondrial membrane damage was observed upon BPA administration in rats.…”