Bisphenol A disrupts gene expression in human placental trophoblast cells

Rajakumar, Chandrew; Guan, Haiyan; Langlois, D A; Cernea, Maria; Yang, Kaiping

doi:10.1016/j.reprotox.2015.03.001

Cited by 45 publications

(32 citation statements)

References 65 publications

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“…This explanation is in accordance with the in vitro study of Zhang et al (2011), who reported that BPA suppressed E2 catabolism in H295R cells but without altering aromatase activity. On the other hand, studies from in vitro and in vivo experiments focused on the impact of BPA on aromatase activity have yielded conflicting results (Castro et al 2013, Rajakumar et al 2015, Shanthanagouda et al 2014.…”

Section: Discussionmentioning

confidence: 99%

Differences in Bisphenol A and Estrogen Levels in the Plasma and Seminal Plasma of Men With Different Degrees of Infertility

Vítků

Sosvorova²,

Chlupáčová³

et al. 2015

Physiol Res

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The general population is potentially exposed to many chemicals that can affect the endocrine system. These substances are called endocrine disruptors (EDs), and among them bisphenol A (BPA) is one of the most widely used and well studied. Nonetheless, there are still no data on simultaneous measurements of various EDs along with steroids directly in the seminal fluid, where deleterious effects of EDs on spermatogenesis and steroidogenesis are assumed. We determined levels of BPA and 3 estrogens using LC-MS/MS in the plasma and seminal plasma of 174 men with different degrees of infertility. These men were divided according their spermiogram values into 4 groups: (1) healthy men, and (2) slightly, (3) moderate, and (4) severely infertile men. Estradiol levels differed across the groups and body fluids. Slightly infertile men have significantly higher BPA plasma and seminal plasma levels in comparison with healthy men (p<0.05 and p<0.01, respectively). Furthermore, seminal BPA, but not plasma BPA, was negatively associated with sperm concentration and total sperm count (-0.27; p<0.001 and -0.24; p<0.01, respectively). These findings point to the importance of seminal plasma in BPA research. Overall, a disruption of estrogen metabolism was observed together with a weak but significant impact of BPA on sperm count and concentration.

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Section: Discussionmentioning

confidence: 99%

Differences in Bisphenol A and Estrogen Levels in the Plasma and Seminal Plasma of Men With Different Degrees of Infertility

Vítků

Sosvorova²,

Chlupáčová³

et al. 2015

Physiol Res

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“…Exposure to BPA may also lead to altered placental function and consequently to pregnancy complications. In studies on trophoblast cells isolated from human placentas and exposed to 0.1-2 μg ml À1 of BPA for up to 24 h, a significantly increased 11β-hydroxysteroid dehydrogenase 2 activity was observed, with a simultaneous increase in aromatase, glucose transporter-1, corticotropin-releasing hormone and human chorionic gonadotropin mRNA levels, while reducing leptin mRNA levels as well (Rajakumar et al, 2015).…”

Section: In Vitro Studiesmentioning

confidence: 98%

Effect of bisphenol A on reproductive processes: A review of in vitro, in vivo and epidemiological studies

Tomza–Marciniak

Stępkowska

Kuba

et al. 2017

J of Applied Toxicology

117

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As bisphenol A (BPA) is characterized by a pronounced influence on human hormonal regulation, particular attention has been aimed at understanding its role in reproductive processes in males and females, as well as on fetal development. Owing to the increasing number of alarming reports on the negative consequences of the presence of BPA in human surroundings, more and more studies are being undertaken to clarify the negative effects of BPA on human reproductive processes. The aim of this work was to collect and summarize data on the influence of BPA exposure on reproductive health. Based on an analysis of selected publications it was stated that there is strong proof confirming that BPA is an ovarian, uterine and prostate toxicant at a level below the lowest observed adverse effect level (50 mg kg À1 bodyweight) as well as a level below the proposed safe level (4 μg kg À1 bodyweight). It seems there is also reliable evidence in relation to the negative effect of BPA on sperm quality and motility. Limited evidence also pertains to the case of the potential of BPA to affect polycystic ovary syndrome occurrence. Although in epidemiological studies this disease was common, in studies on animal models such results were still not confirmed. No unambiguous results of epidemiological studies and with animal models were obtained in relation to the evaluation of associations between BPA and implantation failure in women, evaluation of associations between BPA and sexual dysfunction in men, and impact of BPA on birth rate, birth weight and length of gestation.

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“…The relative abundance of various mRNAs was assessed by a two-step real-time quantitative RT-PCR (qRT-PCR), as described previously (Rajakumar et al 2015). Briefly, 1 mg of total RNA was reverse-transcribed in a total volume of 20 ml using the High Capacity cDNA Archive Kit (Applied Biosystems) following the manufacturer's instructions.…”

Section: Assessment Of Mrna Levels: Real-time Quantitative Rt-pcrmentioning

confidence: 99%

Wisp1 mediates Bmp3-stimulated mesenchymal stem cell proliferation

Cernea¹,

Tang²,

Guan³

et al. 2015

Journal of Molecular Endocrinology

Self Cite

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Adipose tissue expansion, resulting from adipocyte hyperplasia and/or hypertrophy, is a hallmark of obesity. Adipocytes are derived from mesenchymal stem cells (MSCs) through adipogenesis, a process involving three key steps: proliferation, commitment and differentiation. Although studies have elaborated on the mechanisms regulating adipocyte commitment and differentiation, the factors that control MSC proliferation remain largely unknown. Previously, we demonstrated that bone morphogenetic protein 3 (Bmp3), the expression of which was upregulated in our rat model of hyperplasic visceral adiposity, potently stimulated MSC proliferation. In the present study, we investigate the molecular target of Bmp3. We conducted DNA microarray analysis on MSCs treated with and without Bmp3 and identified WNT1-inducible signaling pathway protein 1 (Wisp1) as a differentially expressed gene, whose expression was upregulated 3.7-fold by Bmp3. Wisp1 is a proliferative agent in various non-adipose cell types and is implicated in adipogenesis. Therefore, we tested the hypothesis that Wisp1 mediates Bmp3 stimulation of MSC proliferation. We showed that Bmp3 increased the expression of Wisp1 as early as 3 h following Bmp3 treatment in MSCs. Importantly, the upregulated Wisp1 expression preceded Bmp3-induced MSC proliferation, as determined by [ 3 H]-thymidine incorporation.Furthermore, treatment of MSCs with recombinant Wisp1 led to a concentration-dependent increase in [ 3 H]-thymidine incorporation with a maximal increase of 300%. In addition, siRNA-mediated knockdown of Wisp1 expression attenuated Bmp3-induced MSC proliferation. Taken together, our present findings reveal Wisp1 as a novel target of Bmp3 and suggest that the Bmp3/Wisp1 signaling pathway play a key role in MSC proliferation, and consequently adipogenesis.

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Bisphenol A disrupts gene expression in human placental trophoblast cells

Cited by 45 publications

References 65 publications

Differences in Bisphenol A and Estrogen Levels in the Plasma and Seminal Plasma of Men With Different Degrees of Infertility

Differences in Bisphenol A and Estrogen Levels in the Plasma and Seminal Plasma of Men With Different Degrees of Infertility

Effect of bisphenol A on reproductive processes: A review of in vitro, in vivo and epidemiological studies

Wisp1 mediates Bmp3-stimulated mesenchymal stem cell proliferation

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