Bisphenol-A differently affects estrogen receptors-α in estrous-cycling and lactating female rats

Aloisi, Anna Maria; Seta, Daniele Della; Ceccarelli, Ilaria; Farabollini, Francesca

doi:10.1016/s0304-3940(01)02092-4

Cited by 68 publications

(29 citation statements)

References 21 publications

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“…A study of nonpregnant or lactating Sprague-Dawley rats examined the effects of high doses of BPA (40 mg/kg/d) on ERa immunoreactive cells in the MPOA, ventromedial hypothalamus, and arcuate nucleus. Although lactating females had fewer ERa-expressing cells in the MPOA compared with nonlactating females, there were no significant effects of BPA in the lactating group (86). Here, the response to BPS we observed in the MPOA in F0 females supports the idea that pregnancy/lactation represents a vulnerable period for exposures and that the effects may be "activational" (e.g., only occurring during the period of exposure) (87)(88)(89).…”

Section: Discussionsupporting

confidence: 72%

Bisphenol S (BPS) alters maternal behavior and brain in mice exposed during pregnancy/lactation and their daughters

Catanese

Vandenberg

2016

Endocrinology

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Estrogenic endocrine disrupting chemicals have been shown to disrupt maternal behavior in rodents. We investigated the effects of an emerging xenoestrogen, bisphenol S (BPS), on maternal behavior and brain in CD-1 mice exposed during pregnancy and lactation (F0 generation) and in female offspring exposed during gestation and perinatal development (F1 generation). We observed different effects in F0 and F1 dams for a number of components of maternal behavior, including time on the nest, time spent on nest building, latency to retrieve pups, and latency to retrieve the entire litter. We also characterized expression of estrogen receptor a in the medial preoptic area (MPOA) and quantified tyrosine hydroxylase immunoreactive cells in the ventral tegmental area, 2 brain regions critical for maternal care. BPS-treated females in the F0 generation had a statistically significant increase in estrogen receptor a expression in the caudal subregion of the central MPOA in a dose-dependent manner. In contrast, there were no statistically significant effects of BPS on the MPOA in F1 dams or the ventral tegmental area in either generation. This work demonstrates that BPS affects maternal behavior and brain with outcomes depending on generation, dose, and postpartum period. Many studies examining effects of endocrine disrupting chemicals view the mother as a means by which offspring can be exposed during critical periods of development. Here, we demonstrate that pregnancy and lactation are vulnerable periods for the mother. We also show that developmental BPS exposure alters maternal behavior later in adulthood. Both findings have potential public health implications. (Endocrinology 158: 516-530, 2017)

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Section: Discussionsupporting

confidence: 72%

Bisphenol S (BPS) alters maternal behavior and brain in mice exposed during pregnancy/lactation and their daughters

Catanese

Vandenberg

2016

Endocrinology

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“…This could be explained by a different affinity of BPA (with respect to estradiol) with estrogen α-receptors (8) and β-receptors (20,21). Our previous experiments have demonstrated that exposure of female rats in adulthood to BPA at the same dosage as in the present experiment, modifies the number of estrogen α-receptors in the brain, thus proving an effect on the central nervous system (22).…”

Section: Discussionsupporting

confidence: 49%

Effects of perinatal exposure to bisphenol A on sociosexual behavior of female and male rats.

Farabollini

Porrini

Seta

et al. 2002

Environ Health Perspect

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187

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Perinatal action of estrogens or aromatizable steroids at the central nervous system level is responsible for brain sexual differentiation. Through early contact with the central nervous system, the estrogenic compound bisphenol A (BPA) could alter the processes affecting sociosexual behavior. To test this hypothesis, we studied agonistic and sexual behavior of adult female and male rats whose mothers were administered BPA (40 microg/kg/day) during pregnancy or lactation. An intruder test revealed in males but not in females an increase in defensive behavior due to BPA. We studied the effect of BPA on sexual behavior by testing sexual orientation and sexual activity. Male sexual orientation toward a stimulus female was not affected by BPA, whereas the sexual activity test revealed a slight impairment of sexual performance due to BPA in terms of latency and frequency of intromissions. In females, BPA produced a small increase in sexual motivation and receptive behavior. In conclusion, BPA administration, both during pregnancy and during lactation, does not masculinize female behavior or potentiate masculinization processes of males. On the contrary, we observed a potentiation of female behavior in females and a depotentiation of male behavior in males.

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“…Moreover, differential promoter activity has been shown to be a key regulator of tissue-specific ERa expression (Kato et al 1998, Donaghue et al 1999. Here, we suggest that the alternative promoter usage of the ERa gene is regulated by BPA exposure in a dose-sensitive manner and therefore this mechanism could be, at least in part, responsible for the different and sometimes contradictory effects observed in BPA action on gene transcription (Khurana et al 2000, Kwon et al 2000, Aloisi et al 2001, Ramos et al 2003, Patisaul et al 2006. Since sexual differentiation of the hypothalamus is estrogen-dependent and mediated at least partly through ERa (Simerly et al 1997), BPA deregulation of this receptor could affect the natural estrogen-directed dimorphisms of POA circuits.…”

Section: Discussionmentioning

confidence: 76%

Neonatal exposure to bisphenol A modifies the abundance of estrogen receptor α transcripts with alternative 5′-untranslated regions in the female rat preoptic area

Monje¹,

Varayoud²,

Luque³

et al. 2007

Journal of Endocrinology

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The xenoestrogen bisphenol A (BPA) is commonly ingested by humans. We examined the effects of neonatal exposure to low versus high doses of BPA over the control of estrogen receptor a (ERa) expression in the preoptic area (POA) of prepubertal female rats. Pups received s.c. injections every 48 h of BPA (high dose, 20 mg/kg and low dose, 0 . 05 mg/kg) or diethylstilbestrol (DES, 0 . 02 mg/kg) from postnatal day (PND) 1 to PND7 and were killed at PND8 or PND21. Relative expression of ERa transcripts containing alternative 5 0 -untranslated regions OS, ON, O, OT, and E1 in POA were evaluated by RT-PCR. Methylation status of ERa promoters was determined by bisulfited DNA restriction analysis and ERa protein by immunohistochemistry. In PND8, the high dose of BPA and DES diminished total ERa mRNA levels, mediated by the decreased expression of ERa-O and ERa-OT variants. In contrast, the low dose of BPA augmented total ERa mRNA by increasing the expression of the ERa-E1 variant. In PND21, both BPA doses increased total ERa mRNA by means of the augmented expression of ERa-O and ERa-OT variants. In PND21, the methylation status of the ERa promoters and the circulating levels of estradiol were similar in all experimental groups. At PND8 and PND21, DES and the high dose of BPA decreased, while the low dose of BPA increased ERa protein in the POA. These findings show that neonatal BPA exposure alters the abundance of hypothalamic ERa transcript variants and protein in a dose-dependent manner.

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Bisphenol-A differently affects estrogen receptors-α in estrous-cycling and lactating female rats

Cited by 68 publications

References 21 publications

Bisphenol S (BPS) alters maternal behavior and brain in mice exposed during pregnancy/lactation and their daughters

Bisphenol S (BPS) alters maternal behavior and brain in mice exposed during pregnancy/lactation and their daughters

Effects of perinatal exposure to bisphenol A on sociosexual behavior of female and male rats.

Neonatal exposure to bisphenol A modifies the abundance of estrogen receptor α transcripts with alternative 5′-untranslated regions in the female rat preoptic area

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