Bisphenol A and its analogs bisphenol B, bisphenol F, and bisphenol S: Comparative in vitro and in vivo studies on the sperms and testicular tissues of rats

Ullah, Asad; Pirzada, Madeeha; Jahan, Sarwat; Ullah, Hanif; Shaheen, Ghazala; Rehman, Humaira; Siddiqui, Mariyam Fatima; Butt, Maisra Azhar

doi:10.1016/j.chemosphere.2018.06.089

Cited by 159 publications

(109 citation statements)

References 63 publications

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“…Recent studies have demonstrated that bisphenols alter steroid signalling pathways, having negative effects on male and female reproduction. Our observations did not reveal hormonal changes, even after higher BPS exposure, whereas comparable doses were found to be effective in rats (6). For example, whereas extremely low doses (BPS1: ~0.001 µg BPS/kg bw) affected sperm motility, higher BPS doses (BPS3: ~100 µg/kg bw) showed significant effects on testicular tissues.…”

Section: Discussioncontrasting

confidence: 84%

Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice

Řimnáčová

Štiavnická

Moravec

et al. 2020

Preprint

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Background Bisphenol S (BPS) is increasingly used as a replacement for bisphenol A in the manufacture of products containing polycarbonates and epoxy resins. However, further studies of BPS exposure are needed for the assessment of health risks to humans. In this study we assessed the potential harmfulness of low-dose BPS on reproduction in male mice. Methods To simulate human exposure under experimental conditions, 8-week-old outbred ICR male mice received 8 weeks of drinking water containing a broad range of BPS doses [0.001, 1.0, or 100 µg/kg body weight (bw)/day, BPS1-3] or vehicle control. Mice were sacrificed and testicular tissue taken for histological analysis and protein identification by nano-liquid chromatography/mass spectrometry (MS) and sperm collected for immunodetection of acetylated lysine and phosphorylated tyrosine followed by protein characterisation using matrix-assisted laser desorption ionisation time-of-flight MS (MALDI-TOF MS). Results The results indicate that compared to vehicle, 100 µg/kg/day exposure (BPS3) leads to 1) significant histopathology in testicular tissue; and, 2) higher levels of the histone protein γH2AX, a reliable marker of DNA damage. There were fewer mature spermatozoa in the germ layer in the experimental group treated with 1 µg/kg bw (BPS2). Finally, Western blot and MALDI-TOF MS studies showed significant alterations in the sperm acetylome and phosphorylome in mice treated with the lowest (BPS1) exposure (0.001 µg/kg/day). Conclusions In summary, this range of qualitative and quantitative findings in young male mice raise the possibility that relatively low doses of BPS may impair mammalian reproduction through epigenetic modifications of sperm proteins.

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Section: Discussioncontrasting

confidence: 84%

Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice

Řimnáčová

Štiavnická

Moravec

et al. 2020

Preprint

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“…The weight of evidence should provoke serious policy discussions to protect public health and regulate chemicals considered as EDCs. So far, replacement molecules have led to similar negative observations (Shi, Sekulovski, MacLean, & Hayashi, ; Ullah et al, ) confirming the undeniable fact that urgent measures in global management of the environment and food production are now required to protect the fertility of future generations. Scientists have to find a way to “wake up” the conscience of the politicians, probably via a increased “interventionism” in the media, as it is the case for global warming.…”

Section: Resultsmentioning

confidence: 75%

The negative impact of the environment on methylation/epigenetic marking in gametes and embryos: A plea for action to protect the fertility of future generations

Ménézo

Dale²,

Elder

2019

Molecular Reproduction Devel

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Life expectancy has increased since World War II, and this may be attributed to several aspects of modern lifestyles. However, now we are faced with a downturn, which seems to be the result of environmental issues. This paradigm is paralleled with reduced human fertility, decreased sperm quality, increased premature ovarian failure, and diminished ovarian reserve syndromes. Endocrine disruptor chemicals and other toxic chemicals, herbicides, pesticides, plasticizers, to mention a few, are a rising concern in today's environment. Some of these are commonly used in the domestic setting: cleaning material and cosmetics and they have a known impact on epigenesis and imprinting via perturbation of methylation processes. Pollution from polyaromatic hydrocarbons, particulate matter <10 and <2.5 μm, and ozone released into the air, all affect fertility. Poor food processing management is a source of DNA adduct formation, which impairs the quality of gametes. An important concern is the nanoparticles that are present in food and are thought to induce oxidative stress. Now is the time to take a step backward. Global management of the environment and food production is required urgently to protect the fertility of future generations.

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“…For example, BPA has very low relative binding affinity to ERα and ERβ (~1000-fold less than E2; Kuiper et al 1997). However, exposure to low doses of BPA results in a variety of adverse effects in rodent in vivo and in vitro studies such as altered development of fetal testes tissue, decreased testosterone secretion in adult testis, altered ovarian cyclicity, and altered brain hormone levels and structure from developmental exposure (Richter et al 2007;Eladak et al 2015;Ullah et al 2018;Viguié et al 2018). Although the mechanism of action for BPA that leads to these effects is not entirely defined, it appears that BPA can act potently through nonclassical estrogen pathways (Alonso-Magdalena et al 2012) as well as alter expression of critical steroidogenesis enzymes such as aromatase (Viguié et al 2018).…”

Section: Eac Modes Of Actionmentioning

confidence: 99%

Factors Affecting Sampling Strategies for Design of an Effects‐Directed Analysis for Endocrine‐Active Chemicals

Brennan

Gale

Alvarez

et al. 2020

Enviro Toxic and Chemistry

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Effects‐directed analysis (EDA) is an important tool for identifying unknown bioactive components in a complex mixture. Such an analysis of endocrine‐active chemicals (EACs) from water sources has promising regulatory implications but also unique logistical challenges. We propose a conceptual EDA (framework) based on a critical review of EDA literature and concentrations of common EACs in waste and surface waters. Required water volumes for identification of EACs under this EDA framework were estimated based on bioassay performance (in vitro and in vivo bioassays), limits of quantification by mass spectrometry (MS), and EAC water concentrations. Sample volumes for EDA across the EACs showed high variation in the bioassay detectors, with genistein, bisphenol A, and androstenedione requiring very high sample volumes and ethinylestradiol and 17β‐trenbolone requiring low sample volumes. Sample volume based on the MS detector was far less variable across the EACs. The EDA framework equation was rearranged to calculate detector “thresholds,” and these thresholds were compared with the literature EAC water concentrations to evaluate the feasibility of the EDA framework. In the majority of instances, feasibility of the EDA was limited by the bioassay, not MS detection. Mixed model analysis showed that the volumes required for a successful EDA were affected by the potentially responsible EAC, detection methods, and the water source type, with detection method having the greatest effect on the EDA of estrogens and androgens. The EDA framework, equation, and model we present provide a valuable tool for designing a successful EDA. Environ Toxicol Chem 2020;39:1309–1324. © 2020 SETAC

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Bisphenol A and its analogs bisphenol B, bisphenol F, and bisphenol S: Comparative in vitro and in vivo studies on the sperms and testicular tissues of rats

Cited by 159 publications

References 63 publications

Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice

Low doses of Bisphenol S affect post-translational modifications of sperm proteins in male mice

The negative impact of the environment on methylation/epigenetic marking in gametes and embryos: A plea for action to protect the fertility of future generations

Factors Affecting Sampling Strategies for Design of an Effects‐Directed Analysis for Endocrine‐Active Chemicals

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