Bisphenol A analogues bisphenol B, bisphenol F, and bisphenol S induce oxidative stress, disrupt daily sperm production, and damage DNA in rat spermatozoa: a comparative in vitro and in vivo study

Ullah, Asad; Pirzada, Madeeha; Jahan, Sarwat; Ullah, Hanif; Khan, Muhammad Jamil

doi:10.1177/0748233719831528

Cited by 69 publications

(33 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study reported that BPB exposure induced DNA damage in sperm cells (Ullah et al, 2019). Consistently, BPB exposure resulted in DNA damage in oocytes as indicated by positive γ.H2A.X spot in our study.…”

Section: Discussionsupporting

confidence: 67%

Bisphenol B Exposure Disrupts Mouse Oocyte Meiotic Maturation in vitro Through Affecting Spindle Assembly and Chromosome Alignment

Zhang

Ding

Ahmad

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Bisphenol B (BPB), a substitute of bisphenol A (BPA), is widely used in the polycarbonate plastic and resins production. However, BPB proved to be not a safe alternative to BPA, and as an endocrine disruptor, it can harm the health of humans and animals. In the present study, we explored the effects of BPB on mouse oocyte meiotic maturation in vitro. We found that 150 μM of BPB significantly compromised the first polar body extrusion (PBE) and disrupted the cell cycle progression with meiotic arrest. The spindle assembly and chromosome alignment were disordered after BPB exposure, which was further demonstrated by the aberrant localization of p-MAPK. Also, BPB exposure increased the acetylation levels of α-tubulin. As a result, the spindle assemble checkpoint (SAC) was continuously provoked, contributing to meiotic arrest. We further demonstrated that BPB severely induced DNA damage, but the ROS and ATP production were not altered. Furthermore, the epigenetic modifications were changed after BPB exposure, as indicated by increased K3K9me3 and H3K27me3 levels. Besides, the pattern of estrogen receptor α (ERα) dynamics was disrupted with a mass gathering on the spindle in BPB-exposed oocytes. Our collective results indicated that exposure to BPB compromised meiotic maturation and damaged oocyte quality by affecting spindle assembly and chromosome alignment, acetylation of α-tubulin, DNA damage, epigenetic modifications, and ERα dynamics in mouse oocytes.

show abstract

Section: Discussionsupporting

confidence: 67%

Bisphenol B Exposure Disrupts Mouse Oocyte Meiotic Maturation in vitro Through Affecting Spindle Assembly and Chromosome Alignment

Zhang

Ding

Ahmad

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…The causative involvement of ROS in the pathological changes induced by exposure to BPA has been demonstrated by virtue of the ability of antioxidants (reduced glutathione and α-tocopherol) to reduce its pathological impact [110]. The related toxicants bisphenol F and bisphenol S have also been shown to disrupt reproductive function via an oxidative mechanism [111,112]. The sperm mitochondria appear to play a key role in the genesis of ROS in this model, as part of a self-perpetuating redox cycle that culminates in DNA damage and the induction of apoptosis [113].…”

Section: Environmental Pollutantsmentioning

confidence: 99%

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

2020

View full text Add to dashboard Cite

This article addresses the importance of oxidative processes in both the generation of functional gametes and the aetiology of defective sperm function. Functionally, sperm capacitation is recognized as a redox-regulated process, wherein a low level of reactive oxygen species (ROS) generation is intimately involved in driving such events as the stimulation of tyrosine phosphorylation, the facilitation of cholesterol efflux and the promotion of cAMP generation. However, the continuous generation of ROS ultimately creates problems for spermatozoa because their unique physical architecture and unusual biochemical composition means that they are vulnerable to oxidative stress. As a consequence, they are heavily dependent on the antioxidant protection afforded by the fluids in the male and female reproductive tracts and, during the precarious process of insemination, seminal plasma. If this antioxidant protection should be compromised for any reason, then the spermatozoa experience pathological oxidative damage. In addition, situations may prevail that cause the spermatozoa to become exposed to high levels of ROS emanating either from other cells in the immediate vicinity (particularly neutrophils) or from the spermatozoa themselves. The environmental and lifestyle factors that promote ROS generation by the spermatozoa are reviewed in this article, as are the techniques that might be used in a diagnostic context to identify patients whose reproductive capacity is under oxidative threat. Understanding the strengths and weaknesses of ROS-monitoring methodologies is critical if we are to effectively identify those patients for whom treatment with antioxidants might be considered a rational management strategy.

show abstract

“…Therefore, over the last few years, there has been a considerable scientific effort to evaluate the safety of BPS, since most people and animals are exposed to it through different routes. Unfortunately, several studies report that BPS has similar androgenic/estrogenic effects in different experimental models [14,15].…”

Section: Introductionmentioning

confidence: 99%

Bisphenol A and Bisphenol S Oxidative Effects in Sheep Red Blood Cells: An In Vitro Study

Baralla

Demontis

Varoni

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

Bisphenols (BPs) are plastic components widely used worldwide and occurring in the environment. Exposure to these compounds is known to be harmful for animals and humans at different levels. The aim of this study was to evaluate and compare the oxidative effects of bisphenol A (BPA) and bisphenol S (BPS) in sheep. Reactive oxygen species (ROS) production and correlated structural alterations in sheep erythrocytes were investigated in vitro. Blood samples from four ewes were collected at fasting from the jugular vein using vacuum collection tubes containing EDTA. For ROS assay in erythrocytes, blood was properly diluted and BPA or BPS was added to obtain final bisphenol concentrations in the range between 1 and 300 μM. 2 ′ ,7 ′ -Dichlorodihydrofluorescein diacetate (H2DCF-DA) 3 μM was added to the samples, and fluorescence was read in four replicates using a microplate reader. To evaluate erythrocyte shape, blood smears of blood treated with the different concentrations of BPS and BPA were prepared. A significant increase in ROS production was observed when concentrations of BPS and BPA increased from 1 to 100 μM ( p < 0.05 ). At the higher concentrations of the two studied BPs (300 μM of BPS and 200-300 μM of BPA), a ROS decrease was observed when compared to the control group ( p < 0.01 ). Erythrocytes’ shape alterations were observed in cells treated with BPS and BPA 200-300 μM 4 hours after the beginning of the treatment. This study confirms that BPA and BPS exhibit oxidative effects on sheep erythrocytes. At higher concentrations, BPA was able to modify erythrocytes’ shape, while BPS altered their membrane as a sign of a protein clustering that could lead to eryptosis. These BPs’ effects are consequent to intracellular ROS increase.

show abstract

Bisphenol A analogues bisphenol B, bisphenol F, and bisphenol S induce oxidative stress, disrupt daily sperm production, and damage DNA in rat spermatozoa: a comparative in vitro and in vivo study

Cited by 69 publications

References 60 publications

Bisphenol B Exposure Disrupts Mouse Oocyte Meiotic Maturation in vitro Through Affecting Spindle Assembly and Chromosome Alignment

Bisphenol B Exposure Disrupts Mouse Oocyte Meiotic Maturation in vitro Through Affecting Spindle Assembly and Chromosome Alignment

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

Bisphenol A and Bisphenol S Oxidative Effects in Sheep Red Blood Cells: An In Vitro Study

Contact Info

Product

Resources

About