Bisoniana XCIII. Growth layers in tooth cement and dentine of European bison and its hybrids with domestic cattle

Klevezal, G. A.; Pucek, Zdzisław

doi:10.4098/at.arch.87-10

Cited by 14 publications

(10 citation statements)

References 4 publications

(4 reference statements)

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“…Low and Cowan (1963) described a technique using a conventional microtome for pieces embedded in paraffin, which have been used with small modifications by authors such as Gilbert (1966), Boozer (1969), Lockard (1972) or Thomas and Bandy (1973). However, because of the time‐consuming process and problems associated with paraffin embedment, such a histological technique is not commonly used nowadays, more common being the freezing microtome‐based technique, both for ungulates (Szabik, 1973; Miller, 1974; Pascal and Castanet, 1978; Rice, 1980; Quéré and Pascal, 1983; Klevezal and Pucek, 1987; Moore et al, 1995), and for carnivores (Sauer et al, 1966; Harris, 1978; Zapata et al, 1995, 1997; Garcíaperea and Baquero, 1999). Nevertheless, while the freezing microtome produces cuts between 15‐ and 20‐µm thick; the conventional microtome can produce preparations as thin as 4 or 5 µm.…”

Section: Resultsmentioning

confidence: 99%

Histological Thin‐Sections: a Method for the Microscopic Study of Teeth in Spanish Red Deer (Cervus elaphus hispanicus)

Azorit¹,

Hervás²,

Analla³

et al. 2002

Anat Histol Embryol

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A method to obtain thin histological sections (4-5 microm thick) from teeth, using a conventional microtome, is described for incisors, molars and canines from Spanish red deer (Cervus elaphus hispanicus). Decalcifying solutions of formic acid and nitric acid at different concentrations were ineffective. This was not the case for a solution of 14% hypochloric acid and polyvinylpyrrolidone (TBD-1(R) of Shandon). However, the time needed to complete decalcification was longer than the time estimated by other authors. Incisors required between 4 and 6 days, molars between 7 and 15 days, and canines between 8 and 12 days. The preparation of histological thin-sections from teeth with a conventional microtome, required some training or prior specialization, because most of defective or lost material occurred during the preliminary stage of the study. However this technique is inexpensive enough to be implemented in any histological laboratory. The preparations obtained are suitable for growth mark observation in dentine and in cementum for dating studies. Nevertheless, they are not suitable for enamel change studies, because the enamel is completely destroyed during the decalcifying process.

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Section: Resultsmentioning

confidence: 99%

Histological Thin‐Sections: a Method for the Microscopic Study of Teeth in Spanish Red Deer (Cervus elaphus hispanicus)

Azorit¹,

Hervás²,

Analla³

et al. 2002

Anat Histol Embryol

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show abstract

“…With the objective of evaluate potential differences between the techniques, besides the histological samples with a conventional microtome, several samples with a freezing microtome yielding sections thicker than 15 μ m were also prepared, from decalcified samples of four incisors. Calcified unstained thin sections, approximately 25–30‐ μ m thick, were also obtained following a methodology similar to that described by Lieberman (1994) and Landon et al (1998).…”

Section: Methodsmentioning

confidence: 99%

“…Some authors state that a percentage of animals higher than 25% with non‐identifiable marks or with scarce sharpness, disallow this technique for age determination (Castanet et al, 1977). Others use different teeth of the same animal or different techniques of teeth cutting (Boozer, 1969; Quéré and Pascal, 1983; McCullough and Beier, 1986; Ohtaishi et al, 1990; Landon et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Growth Marks Observation: Preferential Techniques and Teeth for Ageing of Spanish Red Deer (Cervus elaphus hispanicus)

Azorit

Analla

Hervás³

et al. 2002

Anat Histol Embryol

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A total of 207 teeth belonging to 53 deer (Cervus elaphus hispanicus) from Sierra Morena (South of Spain) were analysed using histological thin sections and/or polished thick sections. Growth marks were observed in dentine and cementum of incisors, molars and canines. Tooth, region of the tooth, and type of preparation giving the best performance in terms of sharpness and interpretation ease of the growth marks, when used for Spanish deer ageing, were identified. The tissue chosen for the study was the cellular cementum of the inter-radicular pad from molars and the acellular cementum of the root from incisors, both observed through longitudinal cuts. Histological sections obtained with a conventional microtome were optimal for a reliable identification of the first growth marks. In sections thicker than 15 microm the first rest line, when observed in cementum can be confused with the cementum-dentine junction; whereas in the polished sections from molars it will not appear at all. Dentine interpretation was more difficult. Its observation should only be accomplished using cross-sections of the molar root. Secondary or accessory marks were visible in every tooth, being those from the molar pad the easiest to distinguish. Canines were the less suitable teeth for this study.

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“…In long-living, large mammals, we can estimate the season of birth for animals with a long period of reproduction (Klevezal and Pucek 1987) and separate out animals born at the end of the reproduction season if reproduction is seasonal (Klevezal and Sokolov 1997).…”

Section: Season Of Birthmentioning

confidence: 99%

Bisoniana XCIII. Growth layers in tooth cement and dentine of European bison and its hybrids with domestic cattle

Cited by 14 publications

References 4 publications

Histological Thin‐Sections: a Method for the Microscopic Study of Teeth in Spanish Red Deer (Cervus elaphus hispanicus)

Histological Thin‐Sections: a Method for the Microscopic Study of Teeth in Spanish Red Deer (Cervus elaphus hispanicus)

Growth Marks Observation: Preferential Techniques and Teeth for Ageing of Spanish Red Deer (Cervus elaphus hispanicus)

Reconstruction of individual life histories of rodents from their teeth and bone

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