This study analyzed the spatial heterogeneity of grassland canopy nitrogen in a tallgrass prairie with different treatments of fire and ungulate grazing (long-term bison grazing vs. recent cattle grazing). Variogram analysis was applied to continuous remotely sensed canopy nitrogen images to examine the spatial variability in grassland canopies. Heterogeneity metrics (e.g., the interspersion/juxtaposition index) were calculated from the categorical canopy nitrogen maps and compared among fire and grazing treatments. Results showed that watersheds burned within one year had higher canopy nitrogen content and lower interspersions of high-nitrogen content patches than watersheds with longer fire intervals, suggesting an immediate and transient fire effect on grassland vegetation. In watersheds burned within one year, high-intensity grazing reduced vegetation density, but promoted grassland heterogeneity, as indicated by lower canopy nitrogen concentrations and greater interspersions of high-nitrogen content patches at the grazed sites than at the ungrazed sites. Variogram analyses across watersheds with different grazing histories showed that long-term bison grazing created greater spatial variability of canopy nitrogen than recent grazing by cattle. This comparison between bison and cattle is novel, as few field experiments have evaluated the role of grazing history in driving grassland heterogeneity. Our analyses extend previous research of effects from pyric herbivory on grassland heterogeneity by highlighting the role of grazing history in modulating the spatial and temporal distribution of aboveground nitrogen content in tallgrass prairie vegetation using a remote sensing approach. The comparison of canopy nitrogen properties and the variogram analysis of canopy nitrogen distribution provided by our study are useful for further mapping grassland canopy features and modeling grassland dynamics involving interplays among fire, large grazers, and vegetation communities. pools available to herbivores [8]. Increased soil temperature in recently burned areas enhances nutrition cycling belowground, and potentially improves plant productivity [9,10].Fire effects on vegetation canopies vary with factors such as plant species, season of the ignition, and the time since the previous burn (fire intervals). Fire is effective for promoting the growth of herbaceous vegetation by suppressing woody plant invasion [11,12]. When prescribed fire coincides with spring plant growth, the effect of fire on the landscape is most evident [1]. Relative to non-growing season prescribed fire, spring fires often reduce the forb species that have initiated growth before the burn, and thus increase the dominance and production of the matrix grass species [13]. Moreover, spring fires tend to advance plant growth and speed up the vegetation phenological development [14]. The fire stimulus to grassland plants is dramatic but transitory. It is typically apparent for the first one or two years after the burn, and it then disappears as the communit...