Bismuth protects against arsenite-induced inhibition of proteoglycan synthesis in cultured vascular endothelial cells

Fujiwara, Yasuyuki; Yamamoto, Chika; Inagaki, Takayuki; Satoh, Masahiko; Kaji, Tatsuru

doi:10.2131/jts.37.837

Cited by 5 publications

(5 citation statements)

References 31 publications

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“…We have previously shown that arsenite decreases the synthesis of both HSPGs and CS/DSPGs in cultured bovine aortic endothelial cells (Fujiwara et al, , 2012. Although the cells predominantly synthesize perlecan and biglycan (Saku and Furthmayr, 1989;Kaji et al, 2000), the PG type(s) of PGs, of which synthesis is inhibited by arsenite, has not been identified.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously shown that arsenite, but not arsenate, decreases the synthesis of both HSPGs and CS/ DSPGs in cultured bovine aortic endothelial cells (Fujiwara et al, , 2012 using diethylaminoethyl-Sephacel column chromatography of radiolabeled PG molecules. However, type(s) of PGs of which synthesis is inhibited by arsenite is unclear.…”

Section: Introductionmentioning

confidence: 98%

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Arsenite inhibits gene expression of perlecan, syndecan-1, -2, -3 and biglycan in cultured vascular endothelial cells

Nakano

Tsuneoka

et al. 2020

Fundam. Toxicol. Sci.

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Arsenic is an environmental pollutant and is a possible risk factor for vascular diseases such as atherosclerosis. Vascular proteoglycans (PGs) are key molecules in the initiation and progression of atherosclerosis. We previously demonstrated that arsenite, but not arsenate, decreases the synthesis of both heparan sulfate proteoglycans (HSPGs) and chondroitin/dermatan sulfate proteoglycans (CS/DSPGs) in cultured vascular endothelial cells. In the present study, we aimed to identify the PG molecules whose expression is decreased by arsenite, using a culture system of bovine aortic endothelial cells. The results indicate that a 24-hr treatment of arsenite significantly decreases the mRNA levels of a large HSPG perlecan, small HSPGs-syndecan-1, -2 and -3-, and a small CS/DSPG biglycan in vascular endothelial cells without nonspecific cell damage; the expression of syndecan-4 mRNA was unaffected by arsenite. The decreased expression of perlecan, syndecan-1 and biglycan genes began after 3 hr of arsenite treatment. However, arsenate did not change the mRNA expression levels of perlecan and biglycan in the cells. These results suggest that the inhibition of synthesis by arsenite occurs in particular types of proteoglycans, i.e. perlecan, syndecan-1, -2, -3, and biglycan in vascular endothelial cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Arsenite inhibits gene expression of perlecan, syndecan-1, -2, -3 and biglycan in cultured vascular endothelial cells

Nakano

Tsuneoka

et al. 2020

Fundam. Toxicol. Sci.

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“…In arterial smooth muscle cells, arsenite inhibited general proteoglycan synthesis, which might play a role of bias in the progression of atherosclerosis and vascular diseases [ 41 ]. In addition, this process is protected by the pretreatment of bismuth [ 42 ]. In the current study, we demonstrated that the relative length of protein-linked glycan residues may be dependent on the ATP in the model of arsenite-treated primary KC.…”

Section: Discussionmentioning

confidence: 99%

Arsenite Regulates Prolongation of Glycan Residues of Membrane Glycoprotein: A Pivotal Study via Wax Physisorption Kinetics and FTIR Imaging

Lee

Hsu

Huang

et al. 2016

IJMS

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Arsenic exposure results in several human cancers, including those of the skin, lung, and bladder. As skin cancers are the most common form, epidermal keratinocytes (KC) are the main target of arsenic exposure. The mechanisms by which arsenic induces carcinogenesis remains unclear, but aberrant cell proliferation and dysregulated energy homeostasis play a significant role. Protein glycosylation is involved in many key physiological processes, including cell proliferation and differentiation. To evaluate whether arsenite exposure affected protein glycosylation, the alteration of chain length of glycan residues in arsenite treated skin cells was estimated. Herein we demonstrated that the protein glycosylation was adenosine triphosphate (ATP)-dependent and regulated by arsenite exposure by using Fourier transform infrared (FTIR) reflectance spectroscopy, synchrotron-radiation-based FTIR (SR-FTIR) microspectroscopy, and wax physisorption kinetics coupled with focal-plane-array-based FTIR (WPK-FPA-FTIR) imaging. We were able to estimate the relative length of surface protein-linked glycan residues on arsenite-treated skin cells, including primary KC and two skin cancer cell lines, HSC-1 and HaCaT cells. Differential physisorption of wax adsorbents adhered to long-chain (elongated type) and short-chain (regular type) glycan residues of glycoprotein of skin cell samples treated with various concentration of arsenite was measured. The physisorption ratio of beeswax remain/n-pentacosane remain for KC cells was increased during arsenite exposure. Interestingly, this increase was reversed after oligomycin (an ATP synthase inhibitor) pretreatment, suggesting the chain length of protein-linked glycan residues is likely ATP-dependent. This is the first study to demonstrate the elongation and termination of surface protein-linked glycan residues using WPK-FPA-FTIR imaging in eukaryotes. Herein the result may provide a scientific basis to target surface protein-linked glycan residues in the process of arsenic carcinogenesis.

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“…The metabolic role of Bi, as mentioned, has not been defined. Bi is an effective antidote for the adverse effects of As, mentioned above [20]. The protective role of Bi begins 24 hours after its administration.…”

Section: Annals Of Agricultural and Environmental Medicinementioning

confidence: 99%

Impact of the oxidative and enzymatic metals in degenerated intervertebral disc disease

Dąbrowski

Zioła-Frankowska

Adamek

et al. 2021

Ann Agric Environ Med.

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Introduction. The degenerative process of the intervertebral disc is a heterogeneous process that may exist in two forms, and involves dominant degenerative changes within the nucleus pulposus and the annulus fibrosus. In degenerative disc disease, the oxidative stress factor can play an important role. Objective. The aim of research was to present a new approach to understanding the role of the analyzed elements in the process of degeneration of the intervertebral disc. Materials and method. Selected elements from oxidative groups (Fe, Zn, Mo, As, Se), associated with enzymatic processes (Fe, Mo, Se, Zn, Ag, As, Bi), metals (Fe, Zn, Mo, Li) and metalloids (As, Bi) and their content was analyzed depending on the changes in the radiological images of the intervertebral disc. Elemental content analysis was performed by Inductively Coupled Plasma Mass Spectrometry analytical technique. Results. The similarity between Fe and Se has been demonstrated during different stages of the analysis of groups of patients with degenerative disc disease. There was a negative correlation between Li and degenerative disc disease. The results also suggest that Fe and Ag are involved in degenerative changes within the intervertebral disc. A potential relationship between As/Bi and Fe/Mo in the degeneration of the intervertebral disc was demonstrated. Conclusions. Only some of the correlations can be explained by the metabolism of morphological elements of the intervertebral disc. The relationships indicate new directions for further studies on the degeneration process of the intervertebral disc. The presented study may reflect metabolic changes in the intervertebral disc and adjacent structures in response to the progressive degenerative process.

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Bismuth protects against arsenite-induced inhibition of proteoglycan synthesis in cultured vascular endothelial cells

Cited by 5 publications

References 31 publications

Arsenite inhibits gene expression of perlecan, syndecan-1, -2, -3 and biglycan in cultured vascular endothelial cells

Arsenite inhibits gene expression of perlecan, syndecan-1, -2, -3 and biglycan in cultured vascular endothelial cells

Arsenite Regulates Prolongation of Glycan Residues of Membrane Glycoprotein: A Pivotal Study via Wax Physisorption Kinetics and FTIR Imaging

Impact of the oxidative and enzymatic metals in degenerated intervertebral disc disease

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